Results 1 to 13 of 13

Thread: Fina and cows..

  1. #1
    got fina?'s Avatar
    got fina? is offline Member
    Join Date
    May 2004
    Location
    East Coast
    Posts
    677

    Fina and cows..

    Two male calves, each given s.c. implantations with 140 mg TBA at
    the base of the right ear, showed a high urinary elimination rate of
    trenbolone (TBOH) (detected fluorometrically). Within 3 hours after
    application relatively high concentrations were measured (50-80 ng/mg
    creatinine); the maximum TBOH level was reached after 10 hours (about
    120 ng/mg creatinine) followed by a sudden drop within two days.
    Additional implantation of estradiol-17ß reduced TBOH excretion very
    slightly (Bouffault, 1977).

    Groups of 3 - 4 bull calves were given s.c. implantations of
    20 mg 3H-estradiol-17ß or 20 mg 3H-estradiol-17ß + 140 mg TBOH.
    TBOH caused a marked delay in estradiol excretion. In calves receiving
    estradiol only, the maximum plasma estradiol-17ß level was 3 nmole/l,
    and 95% of the applied radioactivity was excreted in the urine and
    faeces within 20 days; after more than 31 days radioactivity was no
    longer detectable in the urine or faeces. Calves treated with TBOH
    showed a maximum plasma estradiol-17ß level of 0.33 nmole/l and
    excretion of radioactivity was observed up to 107 days after
    administration; at that time faecal and urinary radioactivity levels
    were still 1.4 - 3 nCi/g (Riis & Suresh, 1976).

    Twelve calves weighing 150 - 200 kg each received s.c. implants
    in the ear containing 200 mg 3H-TBA. Half of the animals were
    sacrificed at 15 days, the other half at 30 days after implantation.
    Blood samples were taken at intervals between dosing and sacrifice. At
    sacrifice, the liver, kidneys, and samples of muscle, fat, and bile
    were taken for analysis. Concentrations of radioactivity in the plasma
    were fairly constant during the experimental period, with mean levels
    of 4 to 5 ng equivalents/ml. Tissue concentrations of radioactivity
    were either similar at 15 and 30 days or were higher at 30 days.



    Highest concentrations were found in the liver (42 and 49 ng
    equivalents/g at 15 and 30 days, respectively). Lower concentrations
    were found in the kidneys (15 - 20 ng equivalents/g) and muscle and
    fat (2 - 3 ng equivalents/g). High concentrations of radioactivity in
    the bile (1073 and 736 ng equivalents/ml at 15 and 30 days,
    respectively) indicate its importance in excretion of this compound.
    Comparison of total and non-volatile radioactivity showed that only a
    small amount of tritiated water was produced. About 10% of the
    radioactivity in the liver samples was extracted by diethyl ether or
    ethyl acetate, and this proportion increased to about 20 - 30%
    following incubation with ß-glucuronidase, indicating the presence of
    a glucuronide(s) (Hawkins et al., 1984).

    Two heifers were given single s.c. implantations with 300 mg
    3H-labelled TBA. One heifer was killed 60 days after implantation;
    the implant was removed from the other heifer after 60 days and the
    animal was killed 16 days later. The H content in the liver, kidneys,
    muscle, and fat varied from 0.5 to 25 ppb. Of these residues, 1 - 5%
    was TBA, TBOH, and trenbolone glucuronide; up to 5% was found in other
    organic-soluble material. Of the remaining radioactivity, about 50%
    was water soluble, and the insoluble residue could be made water
    soluble by treatment with the proteolytic enzymes pepsin and trypsin
    (Ryan & Hoffman, 1978).

    Two heifers were given single s.c. implantations with 300 mg
    3H-labelled TBA. After 60 days the implants, which still contained
    31% of the initial radioactivity, were removed. One heifer was killed
    immediately, the other was maintained for 16 days after implant
    removal and then killed. Ethyl acetate-extractable radioactivity in
    blood plasma could largely be ascribed to TBOH; in most cases no TBA
    was found in plasma. Plasma concentrations during days 1 - 55 after
    dosing were 5 - 13 ppb; after 58 days a large increase in both total
    and nonvolatile radioactivity was observed (17 - 20 ppb). The
    half-lives for plasma disappearance of total and non-volatile
    radioactivity were 32 and 29 days, respectively, during the
    implantation period and 18 and 14 days, respectively, during the
    withdrawal period. Plasma ethyl acetate-extractable radioactivity
    amounted to 10 - 74% of the total radioactivity during days 1 - 55
    after implantation, and this declined to 5% at 16 days after implant
    removal. In the 16 days from implant removal to sacrifice,
    radioactivity decreased by 58% in muscle, 75% in liver, 77% in
    kidneys, and 74% in fat (Chasseaud et al., 1976).

    Heifers (aged 15 months, number not given) were given daily oral
    doses of 0.4 or 8 mg TBA per animal for 9 weeks. After 1 and 2 weeks
    TBA was detected in the urine. Two weeks after drug withdrawal the
    compound was detected in some urine samples, whereas after 3 weeks no
    TBA was detected (Stephany et al., 1976).

    A 14-month-old heifer, after i.v. administration of 10 mg/kg b.w.
    TBA, excreted 80% of the administered radioactivity in the bile during
    the first 24 hours; 3.5% was in the free form, 30% was excreted as
    glucuronides, and 30% as sulfates. Metabolites with the 3-ketotrienic
    structure that were identified in the bile are presented in Figure 2.
    Three compounds that had lost their ketotrienic structure were also
    isolated; these metabolites are presented in Figure 3. Less than 1% of
    the administered radioactivity was isolated as tritiated water
    (Pottier et al., 1978).

    Specimens of muscle from the back and rear leg and specimens from
    the liver were taken from two heifers that had been implanted two
    months earlier with 300 mg 3H-TBA. In addition, bile was collected
    by catheterization of one heifer on days preceding slaughter. The
    radioactivity content of muscle, independent of its location, was
    one-tenth the level in liver, whereas radioactivity levels in the bile
    were 15 times higher than in liver tissue, alpha-TBOH and ß-TBOH
    concentrations were determined by reverse isotopic dilution. On
    average, the concentration of ß-TBOH was 0.05 to 0.1 ppb in various
    tissues, whereas that of alpha-TBOH, which was only 0.005 ppb in the
    muscle, reached 0.88 ppb in the liver. Following enzymolysis, ß-TBOH
    was not detected in the bile, which contained, by contrast, nearly
    200 ppb alpha-TBOH. Thus, alpha-TBOH represented 10% of total TBOH in
    muscle, 90 - 95% in the liver, and more than 99% of the total in bile
    (Pottier, 1979).

    3H-TBA was implanted in the ears of two heifers (300 mg;
    388 mCi) and the distribution of the radioactivity in liver and muscle
    tissue was determined, applying rigorously standardized organic or
    aqueous extraction procedures, either directly or following enzymatic
    hydrolysis and proteolytic procedures. These steps yielded almost 100%
    recovery of the radioactivity and indicate that only 5 to 15% of the
    total residues were extractable with organic solvents. The remaining
    radioactivity was either soluble in aqueous media or remained bound to
    tissue structures. In another experiment, liver tissue from a calf
    treated with 3500 mg TBA 68 days prior to slaughter was examined by
    applying radioimmunoassay techniques to determine TBA/TBOH ratios.
    Trienic-steroid type residues were obtained only from fractions
    containing residues extractable with organic solvents (Hoffman et al.,
    1984).


    I had fun reading so i posted it....

  2. #2
    Froggy's Avatar
    Froggy is offline Member
    Join Date
    Feb 2004
    Location
    In the Fog with birddog
    Posts
    725
    Yep...okay...thanks...
    I wish "they" would stop putting estrogen in my beef....

  3. #3
    got fina?'s Avatar
    got fina? is offline Member
    Join Date
    May 2004
    Location
    East Coast
    Posts
    677
    Theres another part i didnt post.

    It says they tested trenbolone on a baby calf and about 10 sec after implant the calf started caughing...........

    MMMMMMM very wierd stuff........

  4. #4
    crizoc's Avatar
    crizoc is offline Banned
    Join Date
    Jun 2005
    Location
    Jacksonville, FL
    Posts
    146
    wowwww

  5. #5
    Mesomorphyl's Avatar
    Mesomorphyl is offline Smart Ass Member
    Join Date
    Jun 2004
    Location
    Pissing on saluu
    Posts
    5,415
    Quote Originally Posted by got fina?
    Theres another part i didnt post.

    It says they tested trenbolone on a baby calf and about 10 sec after implant the calf started caughing...........

    MMMMMMM very wierd stuff........
    You should post it if they give a reason for the coughing or at least if they speculate... Hopefully they killed that thing right then and there and did a autopsy on the lungs and blood samples.

  6. #6
    Mesomorphyl's Avatar
    Mesomorphyl is offline Smart Ass Member
    Join Date
    Jun 2004
    Location
    Pissing on saluu
    Posts
    5,415
    Quote Originally Posted by Froggy
    Yep...okay...thanks...
    I wish "they" would stop putting estrogen in my beef....
    trenbolone given to feelot heafers does not convert to estrogen, but it did show a decrease in estrogen and a slower release thereof even if introduced from an outside source(implantation)
    Last edited by Mesomorphyl; 06-24-2005 at 02:15 PM.

  7. #7
    Froggy's Avatar
    Froggy is offline Member
    Join Date
    Feb 2004
    Location
    In the Fog with birddog
    Posts
    725
    Quote Originally Posted by Mesomorphyl
    trenbolone given to feelot heafers does not convert to estrogen, but it did show a decrease in estrogen and a slower release thereof even if introduced from an outside source(implantation)
    "They"...can put all the tren in my beef they want...but...as a man...I really wish they would stop the estrogen...
    Chicken toooo....

  8. #8
    got fina?'s Avatar
    got fina? is offline Member
    Join Date
    May 2004
    Location
    East Coast
    Posts
    677
    LOL, no the caughing part was a joke.

    But the reason why i put this up was because its interesting to see the effect it has on animals so u could maybe kinda see the damage its doing to us.
    Depending on the person i ask or the place i read up on Tren i get mixed feelings on the negative and toxic effects it has on the human body.

  9. #9
    chris2wire is offline Member
    Join Date
    Mar 2005
    Posts
    640
    Quote Originally Posted by Mesomorphyl
    trenbolone given to feelot heafers does not convert to estrogen, but it did show a decrease in estrogen and a slower release thereof even if introduced from an outside source(implantation)
    Fina doesnt convert, but synovex (which is more widely used now) does have estradiol in it. This makes the heafers grow much more.

  10. #10
    Join Date
    May 2005
    Location
    University Ave
    Posts
    506
    Quote Originally Posted by got fina?

    A 14-month-old heifer, after i.v. administration of 10 mg/kg b.w.
    TBA, excreted 80% of the administered radioactivity in the bile during
    the first 24 hours; 3.5% was in the free form, 30% was excreted as
    glucuronides, and 30% as sulfates. Metabolites with the 3-ketotrienic
    structure that were identified in the bile are presented in Figure 2.
    Three compounds that had lost their ketotrienic structure were also
    isolated; these metabolites are presented in Figure 3. Less than 1% of
    the administered radioactivity was isolated as tritiated water
    (Pottier et al., 1978).



    I had fun reading so i posted it....
    Yeah, I had so much fun reading this that I thought I would post my favorite paragraph. Although, I am disapointed that you left out figure #2.

  11. #11
    got fina?'s Avatar
    got fina? is offline Member
    Join Date
    May 2004
    Location
    East Coast
    Posts
    677
    heres some more.........


    In two barren cows after s.c. implantation of 300 mg 3H-TBA per
    animal at the base of the ear, slow resorption from the implant
    occurred; the half-life of disappearance from the implant was 68 - 84
    days. About 33% of the radioactivity was extracted in the blood plasma
    over the 3-month period after implantation, 70% of which was accounted
    for by TBOH. The main routes of excretion were via the bile and urine.
    Tissue levels after 3 months were about 1 ppb, except in the liver
    (6.5 ppb) and kidneys (4.5 ppb). Twenty-five percent of the tissue
    radioactivity was extractable, 40% of which was TBOH. In the liver and
    kidneys, however, only 10% was extractable, while in perirenal fat up
    to 88% of the radioactivity was extractable. In perirenal fat 50% of
    the radioactivity was TBA. Radioactivity levels in the implantation
    zone were 8 - 21% of the implanted quantity (Pottier et al., 1973;
    Pottier et al., 1975).

    Slow resorption from s.c. implants of 300 mg 3H-TBA occurred in
    2 lactating cows. The half-life for disappearance from the implant was
    approximately 60 days. About 17% of the radioactivity present in the
    blood plasma over the period of 5 months after implantation was
    extractable. Less than 1% of the radioactivity was excreted in milk.
    Ten percent of the milk radioactivity was extractable and 25% of this
    extractable radioactivity was TBOH. Tissue levels after 5 months were
    about 1 ppb, except in the liver (3.4 ppb) and kidneys (2.7 ppb).
    About 25% of the tissue radioactivity was extractable, except in the
    liver and kidneys (both 10%); about 40% of this extractable
    radioactivity was TBOH. In contrast, 88% of total radioactivity in
    perirenal fat was extractable, of which 50% was TBA. Unchanged TBA was
    found in no other tissues. Radioactivity levels in the implantation
    zone were 8 - 21% of the implanted quantity after 5 months (Pottier
    et al., 1973; Pottier et al., 1975).

    Two steers were given by single s.c. implantations 300 mg
    3H-TBA in combination with 40 mg estradiol; the implants were
    removed 60 days later, at which time 28% of the radioactivity remained
    in them. Ethyl acetate-extractable radioactivity in blood plasma was
    primarily ascribed to TBOH; in most cases no TBA was found in the
    plasma. One animal was killed immediately after removal of the
    implant. Plasma concentrations in this animal declined with half-lives
    of 26 days for both total and non-volatile radioactivity; ethyl
    acetate-extractable radioactivity in the plasma of this animal ranged
    between 3 - 5% of the total radioactivity. In the other animal, which
    was killed 16 days after removal of the implant, plasma concentrations
    declined during days 1 - 60, with half-lives of 50 and 55 days for
    total and non-volatile radioactivity, respectively. In the 16 days
    from implant removal to sacrifice, radioactivity decreased by 46% in
    muscle, 2% in liver and kidneys, and 29% in fat (Chasseaud et al.,
    1976).

    Relay bioavailability

    Groups of 3 rats were fed freeze-dried or ethyl acetate-extracted
    liver, kidney, or muscle obtained from two heifers killed 60 days
    after s.c. implantation with 300 mg 3H-TBA. 3H-TBA levels in the
    heifers averaged 30 ng equivalents/g in the liver, 24 ng equivalents/g
    in the kidneys, and 3.2 ng equivalents/g in muscle. Radioactivity
    excretion during the 3 days after feeding these tissues to rats is
    presented in Table 1 (Hawkins et al., 1979).

    Groups of 3 bile duct-cannulated rats that had been fasted for 24
    hours were fed during 1 hour freeze dried liver, kidney, or muscle
    from the two heifers described in the previous paragraph.
    Radioactivity disposition during 48 hours after feeding of these
    tissues is presented in Table 2 (Hawkins et al., 1979).

    Table 1. Excretion of radioactivity by rats after being fed tissues
    from heifers implanted with 3H-TBA


    Excretion in percent of
    administered radioactivity

    Treatment Tissue Urine Faeces Total


    Freeze-dried tissue Liver 3 81 84
    Kidney 2 93 94
    Muscle 6 85 91

    Extracted tissue Liver 5 78 83
    Kidney 2 103 105
    Muscle 2 73 75



    Table 2. Excretion of radioactivity by bile duct-cannulated rats
    after feeding of tissue from heifers implanted with 3H-TBA


    Excretion in percent of administered radioactivity

    Tissue Bile Urine Faeces GI tract + contents Total


    Liver 7 5 59 2 74
    Kidney 3 1 31 60 95
    Muscle 3 2 56 not detected 61


    Effects on protein binding

    The affinity of alpha-TBOH and ß-TBOH for corticosteroid binding
    globulin, measured in vitro using the human plasma of elderly women,
    was very low, less than 0.1% bound, compared with 10% for testos-
    terone. The affinity of alpha-TBOH and ß-TBOH for testosterone and
    estradiol binding globulin was 1% of that measured for testosterone.
    When alpha-3H-TBOH was incubated in vitro with female human plasma,
    it readily bound to the albumin fraction; only 4% was present
    as free TBOH. The total blood clearance of ß-TBOH was twice that of
    testosterone (Philibert & Moguilewsky, 1983).

    Effects on estradiol-17ß excretion and nitrogen retention

    Cattle

    Plasma residues of estradiol-17ß in cattle were affected by the
    presence of TBA in the s.c. implant. Plasma levels of estradiol-17ß
    remained greater than 0.05 ppb for nine weeks in steers after
    treatment with 200 mg TBA in combination with 40 mg estradiol-17ß,
    whereas the residual levels decreased below 0.05 ppb within 5 weeks
    after implantation of 40 mg estradiol-17ß alone (Heitzman & Hardwood,
    1977).

    Implantation of 40 mg TBA in the dewlap of Friesian bulls
    (11 - 16 weeks of age) did not affect nitrogen retention. Implantation
    of 140 mg TBA in combination with 20 mg estradiol-17ß at the same
    site, however, resulted in a 47% decrease in nitrogen retention
    (van der Wal, 1975).

    Pigs

    Pigs (males, females, and castrated males) were given s.c.
    implantations with either 20 mg estradiol-17ß or 20 mg estradiol-17ß
    in combination with 140 mg TBA. At 5 weeks after implantation, steroid
    estrogens were hardly detectable in the faeces, and serum values for
    estradiol-17ß were very low in both groups. Urine estradiol-17ß levels
    were 6 - 82 µg/l in the estradiol-17ß group and 16 - 135 µg/l in the
    combination group (Kroes et al., 1976a).

    Toxicological studies

    Special studies on carcinogenicity potential

    Rats

    Male Wistar rats (number not specified) were injected i.p.
    with 15 µg/kg b.w. 3H-estradiol-17ß (53.6 Ci/mmole), 19 µg/kg
    b.w. 3H-testosterone (54.0 Ci/mmole), 17 µg/kg b.w. 3H-TBA,
    (57.0 Ci/mmole), or 30 µg/kg b.w. 3H-zeranol (50.0 Ci/mmole), all in
    95% ethanol solution. The animals were sacrificed 16 hours after
    injection and the Covalent Binding Indices (CBI, Lutz, 1979) of the
    chemicals to DNA in the liver were quantitated. The CBIs were 11.4,
    4.80, 5.62, and 1.65 for estradiol-17ß, testosterone, TBA, and
    zeranol, respectively (weak carcinogens have a CBI approx. or equal
    10, Lutz, 1979). The positive control, N-hydroxy-acetylaminofluorene,
    had a CBI value of 262 (Barraud et al., 1983).

    The CBI of TBA as a function of time was measured by
    administering 0.83 mCi (22 - 40 µg/kg b.w.) 3H-TBA i.p. to 8 male
    rats. The animals were killed at 4, 8, 12, 20, 24, 36, 48, and 96
    hours. The highest CBI, 7.82 was obtained after 24 hours; after 96
    hours the CBI was 1.11 (Barraud et al., 1983).

    Treatment of rodents with initiators of liver cancer can give
    rise to phenotypically altered cells which, under suitable conditions,
    will develop into foci of potentially pre-neoplastic cells. These foci
    may either regress or develop into malignant nodules, but because they
    only take a few weeks to become apparent, induction of such foci
    represents a useful short-term indication of tumour-initiating
    capacity.

    alpha-TBOH or ß-TBOH (2.5, 5, or 10 mg/kg b.w.), ethinyl
    estradiol (0.05 mg/kg b.w.), testosterone (10 mg/kg b.w.),
    nitrosomorpholine (25 mg/kg b.w.), or diethylnitrosamine (200 mg/kg
    b.w.) were administered by i.p. injection approximately 18 hours after
    partial hepatectomy to Fisher 344 CDF rats (5 males and 5 females per
    group). Two groups presented only with vehicle and one untreated group
    of 5 males and 5 females each were used as controls. The animals were
    allowed to recover for a further 13 days after treatment with the test
    agent. The animals then were supplied with tap water and powdered diet
    containing 0.02% 2-acetylaminofluorene, except that the diet supplied
    to animals in one of the vehicle control groups contained no
    acetylaminofluorene. Seven days after commencing the new dietary
    regime the animals were treated with carbon tetrachloride at 2 ml per
    kg b.w. by intragastric gavage (animals in the vehicle control group
    not given acetylaminofluorene were not treated with carbon
    tetrachloride). Seven days later the animals were killed by cervical
    dislocation and the livers were removed for microscopic examination.

    Most of the animals showed moderate lethargy and other clinical
    signs for two or three days following the operative procedure, but no
    compound-related adverse signs were evident. No significant
    treatment-related effects on body or liver weight were reported. Only
    animals treated with nitrosomorpholine or diethylnitrosamine showed
    significant increases in liver foci compared with the vehicle control
    or untreated groups.

    None of the steroids examined in this study (including alpha-TBOH
    and ß-TBOH) showed any evidence of inducing pre-neoplastic liver foci
    at the dose levels tested. The authors concluded that none of these
    steroids showed any evidence of being a liver rumour initiator in this
    assay (Allen & Proudlock, 1987).

    Special study on immunoresponse

    Cattle

    Antibody production in male and female calves (about 25 animals
    per group) was investigated after s.c. implantation of placebo
    (lactose), 20 mg estradiol-17ß, 140 mg TBA, or 140 mg TBA + 20 mg
    estradiol-17ß. A slight, non-significant immunodepressive effect was

    seen in male calves treated with either estradiol-17ß or TBA alone. In
    the males treated with the combination, this effect was significant.
    In female calves the immunoresponse was unaffected (Gropp et al.,
    1975).

    Special studies on mutagenicity

    The results of mutagenicity assays on TBOH and TBA are summarized
    in Table 3.

    Special studies on no-hormonal effect levels

  12. #12
    Mesomorphyl's Avatar
    Mesomorphyl is offline Smart Ass Member
    Join Date
    Jun 2004
    Location
    Pissing on saluu
    Posts
    5,415
    Quote Originally Posted by Mesomorphyl
    You should post it if they give a reason for the coughing or at least if they speculate... Hopefully they killed that thing right then and there and did a autopsy on the lungs and blood samples.
    Post the findings on the cough in calves you said occurred 10 seconds after injection/implantation.

    Could give more insight on fina cough...

  13. #13
    KrooC's Avatar
    KrooC is offline Anabolic Member
    Join Date
    Sep 2005
    Posts
    3,137
    i still do not see any info ona calf that wasnt killed after 60/120 days.. wonder what sheep ****er did this research

Thread Information

Users Browsing this Thread

There are currently 1 users browsing this thread. (0 members and 1 guests)

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •