12-14-2005, 01:40 AM #1
Long term detection of AS in urine
Does anyone have any other proof on this? Does this mean by testing the other sulfates found in urine that the detection time would increase?
LONG TERM DETECTION OF ANABOLIC STEROIDS ABUSE IN URINE. LONGEVITY OF SULFATE CONJUGATED METABOLITES.
V.P. Uralets, P.A. Gillette
QUEST Diagnostics, Inc., 7470 Mission Valley Road, San Diego, CA, 92108, USA
Detection retrospectivity of anabolic steroids (AS) was an important issue in doping control in recent years. AS testing is currently performed by GC/MS analysis of urinary steroids liberated from their conjugates after enzymatic hydrolysis with E. coli, which cleaves glucuronides only. This yields the most abundant metabolites conjugated with glucuronic acid. Urinary steroid sulfates are considered less important and usually are not included in routine screen.
Relative abundance and longevity of steroid glucuronides vs. sulfates is studied in this paper for several anabolic steroids: mestanolone, mesterolone, methenolone, methyltestosterone , methandriol, and dehydroepiandrosterone (DHEA). Three major urinary steroid fractions were separated and studied: glucuronides cleaved with E.coli enzyme, sulfates cleaved with H. pomatia enzyme, and sulfates cleavable by solvolysis only. Glucuronides are indeed major metabolites in total urine pool collected after steroid administration. However, their excretion rate is relatively rapid. At a certain point in time, usually three - five days after AS oral administration, concentration of metabolites conjugated as sulfates approaches and then exceeds concentration of glucuronides. Sulfates generally stay longer in the system. They are detectable by routine quadrupole GC/MS for at least two – three times longer than glucuronic metabolites. Thus, addition of sulfates to routine screen by using H. pomatia enzyme for deconjugation improves detection retrospectivity. Sulfatation at early stages of metabolism may also be responsible for longevity of some steroid metabolites excreted as glucuronides: 17-epi methylated steroids, such as epimethendiol metabolite of methandrostenolone . Some new sulfated metabolites are found, which were not previously reported: 3b-O sulfates of 3b-hydroxy metabolites of 17a-methyl synthetic AS; 3b-O sulfate of epietiocholanolone originated from DHEA.
Keywords: retrospective doping detection, anabolic steroids, selection of metabolites.
12-14-2005, 03:48 AM #2
norandrolone still for 18 months
12-15-2005, 09:02 AM #3
So you think only the long acting AS will be affected?
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