Thread: Sweeney interview
07-29-2004, 07:35 PM #1
Session 7: Enhancement 5: Genetic Enhancement of Muscle
H. Lee Sweeney, Ph.D.,
Professor and Chairman of Physiology,
University of Pennsylvania.
CHAIRMAN KASS: All right. I know there are some counsel members with planes to catch, and I don't want us to waste any more of Dr. Sweeney's time. We're delighted to welcome Dr. Lee Sweeney, who is Professor and Chairman of the Department of Physiology at the University of Pennsylvania, who has done just a significant amount of outstanding work on muscle physiology and who is going to speak to us today about the genetic enhancement of skeletal muscle and its performance.
Thank you very much, Dr. Sweeney, for being with us.
Push your button there. There we go.
DR. SWEENEY: Thank you.
Yeah, I'm going to just try to give you in half an hour or so background about some of the work we're doing, and then I'm hoping just to allow you to drive a lot of the discussion because I'll set up some of the issues that I see, but as a scientist, I'm afraid I don't think some of the ethical ramifications through quite to the extent of the discussions I've already heard this morning.
My interest is in disease states of both skeletal and cardiac muscle. I'm going to restrict it to skeletal muscle because I think it gives you a good example of really how gray the boundary is between therapeutics and enhancement when one is starting to think about what can be done with genetic manipulation in adults.
Skeletal muscle is a big target in a person because it makes up the majority of the mass of their body, and it's an interesting tissue in that it has built into it cells called satellite cells which are not differentiated muscle cells, but which are actually cells that are called upon to divide and differentiate and regenerate the skeletal muscle.
So they're not really a resident stem cell population because they're not pleuripotential like a true stem cell, but they're an uncommitted set of cells that can with the proper stimuli be induced to become skeletal muscle. They can become other types of tissue as well, but a fairly limited repertoire.
Now, my interest in this began with years ago the beginning of the whole promise of gene therapy, and of course, the idea at the beginning of gene therapy was really to tackle the simplest of genetic diseases, those that involved single genes and usually the genes being missing or at least defective, which was the cause of a large number of diseases, most of them fairly rare diseases.
The issue, and still the issue, the recent gene therapy really hasn't as quickly progressed as we all would have hoped it would have ten years ago, is because the problems are really finding the right vector for a given tissue, that is, a delivery device to actually get the genetic material into the adult tissue, and then also figuring out how to get it there, the delivery.
So these are still the key stumbling blocks in gene therapy today, although great inroads have been made.
Now, in terms of muscle what is sort of emerging as the best sort of ways to deliver genes to muscle are sort of listed here, and this is as of today. Muscle is actually quite good at taking up so-called naked DNA or just plasmid DNA. This is DNA that is not encapsulated in anything.
When I say quite good, I mean it does it with some efficiency. It's perhaps one or two percent of the cells if you inject DNA into a given muscle would take something up.
So this is not an inefficiency that's useful in correcting a primary genetic disease of the muscle itself. However, this would be useful in terms of getting the muscle to produce a substance that would then be secreted into the blood.
And, in fact, this technique has been shown to be successful in an agricultural setting where a colleague of mine has taken DNA that codes for the growth hormone releasing hormone. This is actually a protein which stimulates the release of growth hormone, and in doing so, he can demonstrate that the pigs will now secrete much larger much larger levels of growth hormone.
Obviously one of the other problems with it is it's transient, but in that sort of setting perhaps it's useful to be transient just to sort of give them a growth boost through some period, and then it will go away, but obviously not something one could think of for permanent sort of genetic correction, and especially not a genetic correction of the muscle itself.
Now, viruses are still the preferred gene delivery vehicles in terms of efficiency. Getting genes into a given tissue are what viruses are engineered to do, and so they can be reengineered to deliver therapeutic genes instead of viral genes.
And probably the best vector for muscle is a virus that's known as AAV, which stands for adeno-associated virus. It's a virus that's unrelated to adenovirus, which has been used, as you're probably aware, in gene therapy trials with complications, and the most severe which took place at my own institution.
But this is a different virus that actually does not cause any known disease in humans. Often it can be found in 20 percent or so of the human population that have been infected by AAV with no consequences that can be demonstrated.
And in the last maybe two years a number of different sort of variants or serotypes of the virus have been isolated, and at least two of them are extremely efficient at targeting skeletal muscle. And so there are now gene therapy trials that have begun and many more that are about to be proposed and started that use this virus to try to get genes into skeletal muscle or in the liver. It turns out that this virus is also very good at targeting the liver, which would be very useful in diseases where one wants something secreted into the blood, like in the case of hemophilia.
There's an ongoing trial now with AAV targeting the liver to get the liver to secrete in this case Factor 9 for patients with Factor 9 deficiency.
Adult stem cells obviously are useful in sort of the idea of helping regenerate the tissue, although I must say that although there are a number of types of adult stem cells that can become muscle, again, efficiency becomes the problem, whether they're muscle or bone marrow derived stem cells. It's very difficult to get a large percentage of the muscle rebuilt using this approach in animal models where it's been attempted.
Nonetheless, we and others see the ability to use adult stem cells now as not in the stem cell sense of rebuilding the tissue, but perhaps in the sense of viewing them as a vector where one would take the adult stem cells in the laboratory, put new genes into the stem cells. That would then allow the tissue that they've incorporated in to secrete a substance that would then affect the surrounding tissue or if it's designed to go into the blood.
So you could sort of put adult stem cells then not only in the tissue regenerating sense, but in the sense of being a vector to carry genes into a tissue, to incorporate into the tissue, then to produce something else in that tissue.
And the advantage that they may have over viruses is one of the easiest systemic delivery. In many disease situations it may be that one could simply put the adult stem cells into the blood and they would home in on the tissue. They would home to the tissue that was actually being damaged by whatever the disease process is, and so it would be a very efficient way of targeting the tissue, which with viruses is more difficult at least at this point in time.
So I'm going to give you an example then of using the adeno-associated viral mediated gene transfer, but the example is actually one that we're now trying to do with adult stem cells, which is why I brought this up, because we think we can accomplish the same thing and deliver it much more simply using stem cells that are bone marrow derived from in this case we're working with both adult human cells, as well as adult rodent derived cells.
Adeno-associated virus, as I said, has the huge advantage for skeletal muscle in that it readily infects it. In fact, this may be the preferred tissue target of this virus at least for various forms of this virus.
It's limiting in that the size of its entire genome is only on the order of about 4.7 kilobases. So this is very small and really smaller than most genes in the human, and so one can only make synthetic genes that code for relatively small proteins. And so one has to be judicious in the choice of what one can attempt to do with this virus.
Delayed onset of expression, perhaps more so than some of the other viruses, but nevertheless, with the more robust infection that one gets with serotype one and five, expression commences within a week of injection of the virus into the tissue or systemic delivery of the virus into the tissue.
There's no viral gene expression. This is one of the big advantages of using this type of vector. You can make the synthetic vector without any viral genes, and because of that, there's no immune response against the virus itself.
Obviously there could still be immune response against whatever the product that you're causing it to make, but an example I'll give you, which is one of the advantages of it, what we're making is not something that's missing from the body, but something that we're just trying to get the body to make more of. And so there's nothing for the immune surveillance to pick up on, and so no possibility of immune response.
It integrates at a low frequency, which is both useful, but also a point of some concern in sort of the regulatory and side effect case. The integration, with any integrating virus there are two things to worry about. One is the possibility of oncogenesis being initiated by an integration event.
We have not seen this in our animal models, and other people have not seen this. So the possibility with this virus seems relatively low because there's some evidence that the integration events of AAV are somewhat site specific, and so not very likely to induce oncogenesis.
The other problem, of course, is whether or not one could get germ line transmission, and compared to something like lentivirus, the ability to do germ line transmission is not zero, but it's fairly low probability of germ line transmission, but again, this depends on the route of administration.
It would be more likely that you might get germ line transmission if you're doing a vascular delivery than of direct injection into the tissue, and the duration of expression, because of the integration, essentially is the life of the nucleus that you infected. And so as long as that cell in that nucleus exists, one will get expression. And so for the animal models that we look at, it's the life of the animal essentially.
Just to show you what efficient means, here's a cross-section through a muscle. So these are now -- if you think of the muscle fibers and muscle cells as very long cylinders, this is now slicing through them so that you basically just see their circumference and not their length.
And as you can see in this example, this is now using AAV-1. Essentially every muscle, well, every muscle in the field is now producing a protein that gives a color and an enzymatic reaction that's developed in the laboratory, a bacterial protein that can be used to give this colormetric readout, and you can see that every muscle fiber in the field is blue, and in fact, one can do vascular injection with this virus, and every fiber and every muscle virtually in the leg of the animal will be blue after vascular administration of a large enough dose.
So the efficiency is extremely high if one puts in enough of the virus.
So potential applications, which is what got me interested in using this in the first place, obviously the initial goal of all gene therapies of this sort was primarily genetic diseases, and for muscle that would mean Duchenne and Becker muscular dystrophy is the most common, but also others, such as the limb-girdle muscular dystrophies, myotonic muscular dystrophy, and whatnot, where one can point to a genetic defect in a single gene as the cause of the disease.
A more difficult problem, but actually in some ways, I mean, biologically a more difficult problem, but in fact, the problem that we focused on initially, which is a very real problem in this society where the society is living to be older and older, is the fact that as we get older our muscle function, our skeletal muscle function diminishes both in size of the muscle as well as the relative strength of the muscle and this is a big problem not only from an ambulatory standpoint, but also from a whole body metabolic standpoint.
If the mass of skeletal muscle drops below a critical threshold, then the whole body metabolism is no longer supported properly because the muscle actually functions not only to move the body, but as an important metabolic organ within the body.
Then the last sort of issue, which is actually a trial, trials have been ongoing in this area, is the use of gene transfer into muscle to get therapeutic proteins in the blood, such as Factor 9 deficiency.
So the initial hemophilia trials with Factor 9 were trying to actually get muscle to secrete Factor 9, but now they've shifted to liver because the liver is just a better organ for secretion into the blood than muscle is, although the muscle is capable of it.
So I want to first tell you about where we started some five years ago, which was looking at this problem that the NIH has coined sarcopenia. I think they coined a term to sort of make it sound more like a disease, probably for congressional purposes, but basically what they're really talking about is this progressive loss of muscle mass in force that essentially begins in the fourth decade life in humans and then progresses throughout.
It's slowed, but it's not prevented by exercise, and obviously as I've already mentioned has negative impacts on health and quality of life. It occurs in all mammals, which is useful because that means all of the laboratory animals one works with undergo the same process, and since they live for a much shorter period of time than humans, their life spans are much contracted.
The whole sort of progression occurs on a time scale that one can approach in the laboratory, and our hypothesis back in '96 or seven when we began this was that in large part we thought that what it was really due to was not inactivity. There had been a lot of discussion that as people got older they were just inactive and that's really the main thing that drove it, but we really thought there was a more fundamental cause, especially since there were studies showing that exercise could slow it down but not stop it.
And that was the fact that the repair mechanisms of skeletal muscle decline as you get older, and this causes the muscle to lose function because it's essentially not being repaired properly, and this goes back to what I said at the beginning, that it has within it a resident population of cells, these so-called satellite cells, that when the muscle is damaged -- and muscle is always damaged as you're using it -- are called upon to repair the muscle and rebuild it.
So this sort of rebuilding process involves some sort of damage signal coming out of the muscle which then activates the satellite cells to begin to proliferate, and they proliferate, then they make the commitment to be muscle, and then they either fuse with the existing muscle to repair it, or if the muscle has been severely damaged, they form new muscle.
So what is involved in this are a number of growth factors, some that drive the process, some that inhibit the process in sort of a yin and yang, but the one that we felt was really the most critical and the one that might be the candidate for what's going wrong in aging is a growth factor called IGF-1, which stands for insulin -like growth factor-1, which in normal muscle is involved in growth. It drives protein synthesis, and it decreases protein degradation, and importantly form the repair standpoint, it stimulates this population of satellite cells to both proliferate and differentiate.
And this is an important fact that it can do both because many of the growth factors will drive proliferation but block differentiation, and so increasing their levels could actually interfere with repair, which has been shown in some cases, but here you have one that has a little built in clock. It will drive proliferation for a while through one pathway, and then it will drive through this pathway, and then it will drive differentiation through another pathway, which it turns on with the delay.
So just the sort of thing you might want to try to drive more successful growth and repair. And the reason we thought it might be a problem in aging is because it's really part of the whole growth hormone IGF-1 axis, which as you know, the signals from the hypothalamus, the growth hormone releasing hormone, that are then taken to the anterior pituitary to stimulate it to produce growth hormone go down with aging.
The levels of growth hormone in the blood go down. The levels of IGF-1 produced by the liver; the liver produces all of the IGF-1 that circulates in the body. All of these levels go down with aging.
And what that means is that the IGF-1 levels in the various tissues of the body will also be diminished with aging.
Note: Nadia Rosenthal's presentation at the IABG indicates that it is the tissue specific form of IGF-1 which is mostly responsible for sattelite differentiaton and proliferation -KP
Now, tissues like muscle and other tissues of the body have two sources of IGF-1. They make it themselves under conditions of either injury or rapid growth, but also they have an IGF-1 input that comes from the liver that's ongoing throughout their life.
And so it's this component in particular that's being lost in the aging animal. And so we sought to essentially replace it by supplementing the amount of IGF-1 that the muscle itself could make.
So the strategy was quite simple that we took. We would use gene delivery into the muscle to give it a synthetic gene to have it produce more IGF-1 so it would not be particularly dependent on the liver for a source of IGF-1.
And then the question was: would that then in muscle promote growth and regenerative pathways and would that, in turn, allow the muscle to function throughout the life of the animal without the aging related loss?
So it's a very simple synthetic gene that we put together using a muscle specific promoter driving the rodent IGF-1, and then it's flanked by the viral ITRs and packaged into the AAV viral capsid, and then just to inject into the animals either a vascular delivery into the leg or a direct injection into specific muscles.
And we could show -- this is just using PCR to detect the existence now of our synthetic gene that four months, nine months, even two years after injection the synthetic gene is present and producing IGF-1 messenger RNA.
So then we asked the question with it: is this going to increase the rate of muscle regeneration and maintain mass and old age? And this is the paper that I included in your packet.
What we showed was that if we injected mice, essentially middle aged mice or late middle age in mice -- mice that live to be about 27 to 30 months in age at least in our colonies, and so we injected them about halfway through their life where they were all just beginning to start losing muscle mass, and then we asked, you know, what would their muscle mass in force and force for cross-section look like when they became old?
And so looking at them at 27 months, which was nearing the end of their life, they normally would have experienced in terms of mass about a 15 to 18 percent drop over that age period compared to a six month old mouse when they're sort of at their peak.
Whereas if we had injected them in middle age, they maintained the same mass or even a little greater than they had when they were younger. The same with the amount of force they were able to produce. Their muscles were able to produce normal force instead of showing the decline in force that they would normally see, and the force for cross-sectional area was maintained, as well.
But the speed of the muscle was maintained and the power output was maintained to an even greater extent because one of the other things that happens as the animals get old, as mammals get old, is they selectively lose their fast and most powerful fiber type.
So skeletal muscle is a heterogeneous tissue in terms of it has some of the fibers in it that are small. Some are big; some are slow; some are fast. We lose the very fastest ones as we get older and preferentially replace them with slower fibers. That's one reason why some of the first athletic things that go are your ability to compete in power events or speed events, because that's the first loss that you experience before you really lose muscle endurance or any of those sorts of properties.
And we were able to prevent that totally. The mice did not lose any of their fast fibers, and they had the same speed and power output when they were 27 month old muscles as they did as animals that were only six months of age.
So from that we were able to conclude that IGF-1 over-expression could prevent all of the hallmarks of age related atrophy and loss of skeletal muscle function in mammalian aging, at least based on the rodent model, and now we're hoping to pursue this in larger animal models.
The skeletal muscle regeneration rate is diminished in old animals, and we showed that in another paper other than the one I showed you, and that seems to be the primary problem, that even if you injure the old muscle, it cannot mount a normal regenerative response, but if you maintain IGF-1 expression, it can maintain a normal repair response, and this also, of course, is this hypothesis that we were looking at.
And also it suggests that one could go about this whole pathway as a therapeutic means of maintaining muscle mass either through the strategy that we used so far in these animals, which is to give them an IGF-1 gene supplement or, as I'll mention at the end, one can think about doing it in other ways that might actually be a little simpler to achieve that we're still evaluating.
This also suggested that maybe in dystrophic muscle where the rate of muscle degeneration or the rate of muscle damage is so high that it exceeds the rate of the muscle to repair it, we wanted to ask the question: if you actually increase the rate of muscle repair by up regulating IGF-1 production, could you slow down the damage, the cumulative damage in these dystrophic animals and maintain their muscle mass?
So that's what we looked at, and here is just now comparing a dystrophic muscle where now we've taken a transgenic approach, but we've also done this with virus, showing that -- this isn't projecting very well, at least not from where I am, but this, the IGF-1 producing tissue shows a lot less of degenerative signs than the dystrophic muscle where there's lots of fragmentation of fibers, lots of clumping, lots of regeneration.
There's infiltration from macrophages because there's an ongoing destruction and inflammatory response in the muscles. Even in the diaphragm, which is virtually destroyed by the time these animals reach about 20 months of age, there's been massive sort of hypertrophy and hyperplasia in the diaphragm. So the diaphragm has become much larger and stronger, and interestingly, the amount of connective tissue.
07-29-2004, 07:36 PM #2
So here one of the big problems is that as the muscle is destroyed, it basically becomes like a rubberband. It's replaced with fibrotic tissue and fat infiltration.
The driving IGF-1 over-expression not only drives more successful regeneration, but it prevented a lot of the fibrosis. So you can see the normal amount of fibrosis is measured by collagen content here in the dystrophic mouse, and we've normalized that and sort of brought that down to sort of normal levels with the IGF-1 over-expression in the MDX mouse versus just IGF-1 over-expression alone or wildtype.
And furthermore, this is now injecting a dye into the blood stream of the animal and let the animal run around, exercise a little bit, and then see if the dye is taken up in the muscles because normally the dye would be excluded because the muscle membrane would be intact.
This is showing that in the dystrophic muscle they're so fragile and being damaged at such a high rate that the dye penetrates quite easily either in the diaphragm or in the muscle that's being used to run at a very high rate, whereas in the same sort of animals, same dystrophic animal that's now over-expressing IGF-1, the fibers are being maintained in such a better state of repair that there's very little dye penetration in either the diaphragm or the leg muscle, suggesting that these muscles are going to be able to be preserved.
And so this is something that we would now like to really look at in large animal models because there are dog models of muscular dystrophy, with the idea of trying to evaluate whether this would be a potential basis for thinking of therapies in humans, and again, either delivery of an IGF-1 gene or some other way of driving this regenerative capacity is a way to think about attacking this general sort of category of human diseases, the muscular dystrophies, and it may not -- you might not even have to understand totally the primary problem if you could just drive the regeneration for some of the muscular dystrophies where it's really still not very clear what the primary problem is.
So this all leads to the idea that this IGF-1 signaling can increase satellite cell proliferation under growth and repair mechanisms that will drive muscle hypertrophy in extreme conditions, even muscle hyperplasia. Hyperplasia means the muscle is actually making more muscle fibers, not just repairing its existing ones or making them larger.
Note: Can you say musclehead?
So I addressed these first two issues that we were interested in, but then it also suggests that IGF-1 over-expression should increase the rate and amount of skeletal muscle growth in young animals, and indeed, we showed that early on that that's true.
If you inject one leg of a mouse and not the other leg while it's in its young adult ages, you can actually show that the muscles of the leg get larger. This is, again, looking at the diameter. It's on the order of about 18 to 20 percent larger, and this is a sedentary animal.
And so this is one leg versus the other leg. Just the only difference here is the injection of the synthetic gene to make IGF-1, and if you do it systemically and look at all of the muscles of the animal, you can see here is a forelimb of an animal where there's no over-expression of IGF-1. Here is IGF-1 over-expression in all of the forelimb muscles, and here at the hind limb.
And so you can see there's pretty massive hypertrophy, again, on the order of 20 to 25 percent overall in the adult animal, and the gains are even larger during the rapid growth phases. So in a young animal that's growing, it may outstrip its age-matched control by as much as 40 percent at a given point in its life in terms of its muscular mass.
So there are a lot of benefits then from IGF-1 over-expression, but from the standpoint of now I've been talking in terms of trying to use it therapeutically, but obviously from what I just showed you, one could think about it in terms of a gene enhancement, in terms of either an animal or a human.
And in terms of a human, those were some of the relevant papers, but in terms of humans, the question that we were asked so often and that has been really since the day we published our first paper on this on people's minds: could this sort of gene transfer into skeletal muscle actually be used for a genetic enhancement of athletic performance or even just cosmetic purposes?
Just say you'd like your pectoralis muscles to be a little larger because you want to look a little better at the beach. Just take a few injections of the virus, and a month later while you're watching television, your muscles have gotten bigger.
So, you know, a lot of implications in terms of genetic enhancement, and so I'll show you a little bit of our attempts to now evaluate this in a rat model. The rats are easy to sort of train. We didn't have much luck trying to make the mice exercise for us, but rats are fairly cooperative.
So we had the rats climb ladders with weights velcroed to their tails, and so fairly large weights that we would increase over the time of the training, and so this is sort of a progressive weight training protocol for eight weeks.
What we did was we took control rats who were not asked to exercise, and we injected the IGF-1 virus into one leg, but not the other, and then we had weight training animals where, again, we injected IGF-1 virus into one leg but not the other, and then we had another group where we went through the weight training and then let the de-train for three months. And three months would normally be enough time to lose all of the benefits from the weight training. This is one of the depressing things about exercise.
You know, you can work as hard as you want for two months straight and then sit back for three months and do nothing, and it's like you never did anything. So we had the rats go through that, too, because we wanted to address whether the IGF-1 would help maintain the mass once you stopped, which would also be of interest in terms of an athletic population or an elderly population.
So what we saw was in terms of the muscle mass -- this is after the eight weeks -- so this is the average mass of the animals that did nothing. In the leg where the IGF-1 was injected, on average the muscles are about 15 percent larger.
In the weight trained animals, they worked very hard. It was really quite a severe weight training exercise, and we were able to induce about a 23 percent increase in mass, and in the animals in their legs that had the IGF-1, they experienced an ever larger increase in mass, up to about 32 percent.
But in terms of the force output of their muscles, it was even a more striking difference in that the IGF-1 injected muscles with no exercise got almost 16, 17 percent stronger on average, whereas the ones that were weight trained were actually no stronger than the animals that had IGF-1 and sat in their cages for the two months. They were about the same strength, but the muscles that had the combination of weight training and IGF-1 were almost 30 percent stronger.
So the effect in mass is not as large as the effect on the overall strength of the muscle, and the reason for that was, in fact, the severe weight training had lowered the sort of force per unit mass of the muscle in the weight trained animals, whereas we had an enhancement in the IGF-1 treated animals, and sort of an intermediate with the weight training and IGF-1 together, and the reason for that is shown. Again, some cross-sections of muscle.
What happened in the exercised animals, and I'm not sure why my slides aren't projecting that well, but what happened there was the weight training was severe enough that we actually had a fair amount of injury and fibrosis in the muscle, and so that's what happens to athletes that can overtrain.
You know, you damage the muscles and you get fibrosis. Then it sort of works against you. It lowers the sort of strength per unit mass of the muscle, whereas the IGF-1 was so effective at the repair that even though the muscle was being massively overloaded, it rebuilt itself and looks just like healthy tissue and had normal sort of force for cross-sectional area.
So a number of benefits, and then the last benefit was when the animal stopped. You can see during the de-training, the weight trained animals went back down, and then after two months, as I said, they're back down almost to where they started before they lifted a single weight, whereas in the muscles that had IGF-1, the decline percentage-wise was a lot smaller, and they ended up with some gain over weight training alone and certainly a gain over just IGF-1 alone.
And so they were able to maintain some of the weight training benefit at least three months after the cessation of the exercise.
So this is sort of a summary of all that I was saying, but the bottom line, just to speed this along a little bit, is that this approach certainly would lead to genetic enhancement of athletic performance because it would increase the rate in amount of skeletal muscle growth with resistance exercise. It would increase the rate and extensiveness of repair following an injury. So you'd be better able to maintain muscle mass, strength, and speed after the training stops and certainly during aging, as we had shown before.
So tremendous benefits from the athletic standpoint I think not the least of which is how rapidly one could come back from an injury and how well one would sustain an injury and get complete repair of the muscle, not to mention that for speed and strength events, one might not see the precipitous fall in performance that normally comes after age 30 even in a training athlete.
So this little bit was what I presented to the World Anti-Doping Association because they're quite concerned about how close we are of genetic engineering or enhancement of athletes actually cropping up in terms of international sporting events.
And you know, as I said to them, I think the real danger of that -- and this is just to acknowledge some of my colleagues -- the real danger of that is not that it's going to happen any time soon in this country because we're still going at a fairly slow rate of trying to just really assess the safety of some of these gene transfer techniques even for treating, you know, primary genetic diseases, rare diseases for which there are no treatments.
And so the availability of this sort of technology to an athlete in this country is not going to happen any time soon, but on the world stage, in a world where countries in the past have shown that they want their athletes to win no matter what and they will give them any experimental drug that might be performance enhancing no matter what the long-term consequences, one can imagine that with enough money you could put together a program to genetically engineer your athletes and do it in such a way, which is what one is really concerned about that it would be totally undetectable unless you were to remove tissue from that athlete. There would be nothing in the blood, no signature in the blood or the urine to indicate that the tissues had been genetically manipulated.
So this is their concern, certainly not a concern, I think, in this country in the short term, but maybe a concern on the world stage maybe even in the next decade.
Just to let you know where we're going a little bit, I alluded to the fact that, you know, we started trying to intervene in this growth and regeneration pathway for aging by driving IGF-1, but what I didn't mention is in the last few years it has become really clear that there are major inhibitors of this whole pathway that the muscle actively is producing to sort of keep it in check.
And one unresolved question and one we're looking at and probably other people are looking at is whether some of these components also could help drive the repair and aging if you could block them. Note: Check out J. Tidball's talk at the IABG 10 on " Mechanisms Regulating Muscle Wasting during Muscle Disuse or Aging".)And so you could imagine there the approach would be either to create a substance in the blood that would interfere with the action of this protein, which has been called myostatin, or you could even imagine a small molecule screen might pick up a selective inhibitor of this protein which is in the -- it's a TGF beta family member.
So this is a target I think you're going to see increasing interest from drug companies, and it may have application in aging. It may not, but it certainly probably does have application in such things as juvenile diabetes and maybe in some of the muscular dystrophies where interfering with the signaling of this protein might allow the muscle to rebuild itself better and stronger.
And that's going to be much easier to implement, and that certainly would be a performance enhancer for an athlete, and those drugs are being developed now, and the accessibility of an athlete to those sorts of drugs might end up in the same sorts of results as what I was showing you for the IGF-1 over-expression.
And certainly in the general population I think this could be used as an instant muscle builder, and the nice thing about a drug is you sort of take until you've got what you want, and then you stop taking it, and it doesn't drive the process indefinitely.
We're going into clinical trial in the next year or so with AAV targeted at a primary muscle disease, a deficiency in what is called the sarcoglycan complex, which causes a form of muscular dystrophy known as limb-girdle muscular dystrophy. These components are all small enough to fit easily into AAV, and so I think the first real clinical test of this virus for sort of directed at a primary muscle problem are going to be in the context of trying to repair this whole structure, which is deficient in limb-girdle muscular dystrophy.
And we'll learn a lot about how easy it's going to be to actually use this gene transfer vector in humans in the process of that, and it's actually a collaboration between myself and groups at Harvard and the NIH and the Généthon in France, which is funded by the French government and the French Muscular Dystrophy Association.
So we're planning to do multiple -- sort of coordinate trials in this country and in France on this disease with the idea of then moving on to other primary muscle diseases and perhaps even looking at the IGF-1 myostatin axis as a possible therapeutic that we could then bring to humans in a muscle disease setting.
So that's my background on what we're doing, and I'd be happy to answer questions about where it's going.
CHAIRMAN KASS: Thank you very much.
Bill May and Dan Foster.
DR. MAY: I betray my ignorance, but I was wondering whether indirectly it might make a contribution to the treatment of women with osteoporosis, both increasing muscle strength, allowing them to increase their engagement in weight exercise that decreases bone loss and protects also against falls because you've got greater stability and strength in the muscles.
DR. SWEENEY: Yeah, I think that's a real possibility. I mean, the IGF-1 has a number of other advantages that I didn't show you data on, and one is that as the animals get older they're not only stronger, but they're leaner. Their body fat is much lower than a normal animal.
And also, the bone loss that the mice undergo seems to be prevented at least in the limb muscles that we've looked at, and we assume it's because they are loading the bones more because of the larger muscle strength, and that does prevent a significant amount, if not all, of the bone loss, at least in the leg muscles of the mice.
CHAIRMAN KASS: Dan Foster.
DR. FOSTER: That was a lovely presentation. I think you've already answered this because you said that if you used enhancement in humans that there was no marker. I was going to ask the question: is there any leakage of IGF-1 from the muscle into the blood? And if so, was this due to a movement from the leg into the liver?
The reason, if you have a persistent elevation of IGF-1, you know, you get a disease from that that's acromegaly, and I gather that there's no leakage and no blood contribution here.
DR. SWEENEY: Yeah, That was part of the design. There are a number of different splice forms of IGF-1, some which lead to ready secretion into the blood and others which are the forms the tissues normally make for themselves, which cause sort of a co-up regulation of the binding proteins that trap the IGF-1 in the tissue.
So we can detect absolutely no elevation in the blood, which was part of the inherent design of what we were trying to do. So the issue would be, you know, we want to look at this in larger animal models obviously before we would try it in a human, and then try massive overdoses of the virus to make sure, you know, at what point we actually do run a danger of exceeding the muscle's ability to trap it and spill it into the blood because that would have a lot of negative consequences.
DR. FOSTER: I just want to ask one real quick technical question. IGF-1 has, you know, effects on -- anti-apoptotic effects. I mean, it's been used to limit infarcts and things of that sort. But it looks like -- I mean, this is just a programmed cell death, you know, a signal death apoptosis, just a signal to die, and probably some of the stuff that he's doing in his muscle for the extra exercise and everything is just apoptotic effects.
And so one of the question would be: do you think that's contributing at all? I mean, I think all of the hypertrophy and everything you thought is not going to make that a major effect.
DR. SWEENEY: Yeah. Well, we've looked at this in the context of disuse atrophy, where you immobilize the animal's leg either in a cast or in sort of a simulated sort of weightlessness, hind limb suspension. Anyway, you don't allow the animal to use the muscle.
And just like if you casted one of our muscles, the animal will lose half of the muscle mass in the order of a month.
The IGF-1 doesn't stop that at all, which is why we were interested in looking at it. So even though it's anti-apoptotic especially in the heart, there's actually in the case of muscle disuse, there's a dominant pathway that blocks IGF-1, that shuts it down so that it doesn't allow it to have its normal anti-apoptotic effect.
We're combining IGF-1 delivery with other mediators of that pathway in skeletal muscle to show that you can then stop all of the loss not just with aging, but if you impose disuse on top of aging, then nothing happens. But the IGF-1 itself is probably anti-apoptotic in certain injury situations, but not in a non-load bearing situation where apoptosis seems to be sort of the dominant pathway that causes that rapid loss of muscle, and IGF-1 is shut down.
CHAIRMAN KASS: Bill Hurlbut.
DR. HURLBUT: Well, I want to ask you since what we're really interested in here is not the technical questions of even a therapeutic approach but the ethical implications. I want to ask you a broad question about what you see as some of the medical or scientific down sides of this and whether this actually holds potential for being a technique that could be casually used.
What I'm thinking of here are you very quickly alluded to the concern that's been raised in other fronts in gene therapy. There may actually be germ line incorporation when it's not suspected.
Daniel's question about systemic effects, obviously you add something into the body and it's going to affect more than just itself, if not directly through diffusible or circulating agents, then maybe just in the allometry, if you will, of proportions. You increase the muscle mass and you've got to have the supporting ligaments and bones to deal with it and so forth.
And then, of course, there are questions of whether it interferes with apoptosis you might induce cancer and these kinds of things, and so what I'm asking you is basically the scientific medical side of this. Is it a realistic concern that this might end up being used by anybody who wasn't just out and out reckless?
DR. SWEENEY: Well, as I said, I think that the technical barriers to doing that are sufficient enough that that just couldn't be casually done in a country such as this where the regulations would prevent it, but I think the real danger of it happening would be precisely what we're seeing, a government sanctioned program in another country where the whole goal of it would be athletic enhancement for the international sports stage.
But what may be something that's more relevant and more of a risk is if, in fact, the pharmaceuticals end up developing drugs with the idea of using them for diabetes or even some of the milder muscular dystrophies that target, say myostatin, and those are accessible, then those drugs are probably going to have a lot of the same effects that I've talked about in terms of muscle.
But depending on how specific they are, they're going to have side effects as you're alluding to. I mean, there the side effects are a little different from IGF-1 side effects. Myostatin is a TGF beta family member, and all of the drugs that exist now probably are going to have some cross-reactivity with the other family members, which will interfere with immune function and a number of other critical functions.
And so, you know, those are going to have to be very tightly regulated substances because, again, someone wanting the muscle enhancing actions could abuse it to the point where just like with anabolic steroids or growth hormone or anything else, you induce side effects.
Now, with the sort of genetic delivery of IGF-1, as I said, we tried to engineer safeguards against some of the issues you've raised in terms of trying to keep it out of the blood. In terms of if a cell that we had transduced became cancerous, the IGF-1 would be shut down immediately because the promoter that's controlling it is only differentiated muscle promoter.
So if the cell transformed, they would shut down. Now, that's different. What is possible is if there were a tumor in the tissue where there were high levels of IGF-1 in the tissue, then it might help the growth of the tumor to some extent, but the levels I'm talking about in the tissue are not 100-fold higher than normal. They are just several-fold, and so this is still, I think -- I think the tumorigenesis aspects are at low risk.
I think the highest risk in this setting would be if we started spilling high levels of IGF-1 into the blood.
DR. HURLBUT: You haven't seen any insertional mutagenesis or anything like that?
And, by the way, I want to ask you also: have you seen an increase in longevity of these mice?
DR. SWEENEY: We've seen a slight increase in longevity, and it's small enough that we haven't got enough animals yet to make it statistically significant, but it might be ten percent or so at the end of the day.
I mean, it appears to us they are living a bit longer, but as I say, because it's a small effect, we haven't had a large enough number.
DR. HURLBUT: So is basically your answer that, to sum it up, you see this as a therapeutic agent in the face of a difficulty, but it would otherwise be very reckless at least in the near term and maybe even in the long term to do this kind of thing?
DR. SWEENEY: Yeah. Well, how reckless it is is going to have to be determined by a lot of safety testing that has yet to be done, and so it's difficult for me to see how reckless it would be in the long term.
I mean, my personal bias is that the general approach, if it can be technically made more -- if it can be done more easily, I think would achieve our goal of in the aging population or in a dystrophic population of in one case improving quality of life and in the other case extending life.
And I think the potential side effects are minimal. The possibility of germ line transmission with the virus is there, although it's a very low probability, and if you're trying to make it happen, you can show some germ line transmission, but in the way I would view it delivered in a therapeutic setting, I think the possibility of germ line transmission is near zero.
But that could change as the viruses are made more efficient to get into muscle. They also might be more efficient in getting into the germ line, and so, you know, the FDA is quite concerned about this, and so we're trying to evaluate with all of these new serotypes whether or not at the same time we've got something that's better for muscle we've also got a higher risk of that type of transmission, and the data is just not there yet to tell you.
07-29-2004, 07:37 PM #3
CHAIRMAN KASS: Let me follow and then Janet.
If you think just about the question of aging and the muscle degeneration, and leaving aside for the moment the question of the safety about which we don't know near enough, but why wouldn't one think of this not so much as species of enhancement as the species of prevention, and in principle, I mean, just to think it through, why isn't this something that one would be thinking that this fit for everybody long term?
DR. SWEENEY: Well, --
CHAIRMAN KASS: In other words, as a preventive measure to be given to people who are 30 years old?
DR. SWEENEY: I mean, and that was sort of our original thinking until it became clear that also, you know, it's also an enhancer. It not only prevents, but it allows you to be stronger and the muscles healthier than they would have been otherwise.
But, you know, if you take it away from the athletic context, which sort of muddies the whole thing, then I think of it -- I do think of it as a preventative measure. I think if the level of safety was absolutely demonstrable that there was zero risk, then I think every person would want to be treated in this way when they're young enough so that, you know, you would never lose muscle function as you got old, I mean, assuming that you could show that there was no down side to it.
At least from my limited viewpoint, I would see it that way, and this is what I had said and actually the popular press article that I gave you. I think if we come to a point where there's no safety issue at all and no specter of germ line transmission or anything else and all you get out of it is you stay strong as you get old so that you can get around and have a better quality of life, it would be hard for me to believe that that wouldn't then gain acceptance.
And when that gains acceptance in the population in general, then, you know, the athletic government agencies are just going to have to deal with it because whatever enhancement it provides to those athletes the public is not going to care about.
CHAIRMAN KASS: Michael.
PROF. SANDEL: Just on that, so if you give it as prevention when someone is 30, does that have the effect of preventing the deterioration of muscle function as they age, or does it bump up where they were before they got their shot at 30 and then keep them at a certain level?
DR. SWEENEY: Yeah. Well, what we've seen in the animals, because we've looked at this, is if you inject them at a point where they're no longer growing at all, they've just leveled off like we would be at 30 or 40 or whatever, if they do nothing, then they don't gain any muscle, but if they exercise at all, they gain a lot more. They start to gain a lot of muscle.
So if you absolutely restrict them so that they really can't do much of anything except sit in their cage, then they get no gain, but they get proportionally more gain the more they're allowed to exercise.
So you know, just a Weekend Warrior could get tremendous gains probably, you know, beyond just not losing muscle. But if they are sedentary and you really restrict them, the answer is that they keep the muscle mass they had the day you injected them and don't go down from there.
But, in fact, if you inject them when they're really old, when they've lost a lot of muscle mass and also don't allow them any exercise, they won't go back up. They'll just stay where they were the day you inject them. They won't go down anymore.
But combining it with exercise, you can really then bump up and get an enhancement.
CHAIRMAN KASS: Janet.
DR. ROWLEY: I'd just like to go back to some more technical questions, and particularly because in your article you did all of this by injecting into the muscles, whereas you also describe research where you did it systemically.
And I'm curious, again, sort of following on with some of Dan's questions as well as Bill's. When you do inject it systemically, particularly in terms of the virus, you're saying that it homes preferentially to skeletal muscle.
So how long does it take to clear the blood? And does some of the virus really go to the liver?
DR. SWEENEY: Yeah, I know that --
DR. ROWLEY: And have systemic effects?
DR. SWEENEY: Yeah, well, now, the liver is the other organ that has a high tropism for this virus. So quite a bit of the virus will go to the liver. None of it will express IGF-1 because the liver can't turn it on.
DR. ROWLEY: Okay. So it's a muscle specific promoter.
DR. SWEENEY: Yeah. So that you can show that the genetic material is in the liver, but it's dormant. There's no way for the liver to activate it.
DR. ROWLEY: Okay. And just looking ahead, you were saying that some of the -- you were thinking of using stem cells from bone marrow as vectors and having them home to the muscle.
DR. SWEENEY: Yeah, they will home preferentially to injured tissue. So if it's a primary muscle disease that's ongoing, they will home to the muscle under those conditions.
DR. ROWLEY: But then that wouldn't be an aging strategy, though you can say aging people have injured muscles. So that --
DR. SWEENEY: No, no. We were thinking of that as more of a way to get delivery, widespread delivery in a dystrophy background, although you can also get the cells to home to the muscle if you exercise the muscles hard enough to induce injury.
So if you run the animals hard on a treadmill for a while, then inject them, the muscles that they've been using and in doing low level damage to is where the stem cells will then home.
DR. ROWLEY: Thank you.
CHAIRMAN KASS: Gil.
PROF. MEILAENDER: I have to really show my ignorance here, but do rodents as they age show signs of dementia? That's something that we would --
DR. SWEENEY: Actually I don't know.
PROF. MEILAENDER: I mean, I was just wondering in terms of what you had said before, which at one level makes sense to me. The issue was, you know, if you solve the safety questions, then it would be hard to know why any of us wouldn't want this just so that we'd be healthier, stronger, and less prone to debilitating injury as we aged.
And that made sense to me, but then I started to think about being so vigorous forever as I was demented.
PROF. DRESSER: I just wondered how elaborate a process it is to produce this. I mean, say, for the sake of argument, everybody does want this. I know you can't predict the price, but is it time intensive to prepare or what?
DR. SWEENEY: Yeah, I mean, the technology to produce it today is certainly not the technology that we would be using by the time we've dealt with all of these issues. I mean, it's gotten to do -- I mean, to do these studies that I've talked about or even to do the dog studies, to treat the legs of a dog would cost a few thousand dollars at this point, but this is with technology that's far from optimized. This is laboratory technology, not scaled up technology.
CHAIRMAN KASS: Now, I'd like to raise a question that won't get any sympathy, but this is in a way the opposite of your question, Gil, where you're talking about the failure of the coordination of the aging of different systems, about which I know a vast collection of jokes, but I will spare you those.
But what's in a way at stake in this is something like the view of the life cycle and, forgive me, but a place of decline in the overall shape of a life, and while nobody from a medical point of view or even from an experiential point of view would choose debility, given the opportunity to avoid it, one at least has to wonder what the world would be like if you've got 75 year old men quite happily playing ice hockey and what the view of the life cycle would be if in a way what you really are aiming for -- never mind the immortality research -- but you're going to get everybody up to the brick wall sort of looking and acting as if they were 30.
And on that subject I promised Michael Sandel this, but there's a wonderful passage from Montaigne which I'd like to put in the record. Let me read this.
The question really was for those people who don't want to add years to life but life to years, of which this would be a great benefit.
All of us, I think, would want this, but the question is whether or not death would become even more of an affront and whether in some ways the fear and loathing of death would be on the increase in the absence of these signs of decline. And here is Montaigne's passage.
Note: WARNING: THE PASSAGE BELOW IS NOT FIT FOR HUMAN CONSUMPTION
"I notice that in proportion as I sink into sickness I naturally enter into a certain disdain for life. I find that I have much more trouble digesting this resolution when I am in health than when I have a fever. Inasmuch as I no longer cling so hard to the good things of life when I begin to lose the use and pleasure of them, I come to view death with much less frightened eyes. This makes me hope that the farther I get from life and the nearer to death the more easily I shall accept the exchange.
"If we fell into such an exchange, namely, decrepitude, suddenly, I don't think we could endure it. But when we are led by nature's hand down a gentle and virtually imperceptible slope bit by bit, one step at a time, she rolls us into this wretched state and makes us familiar with it so that we find no shock when youth dies within us, which in essence and in truth is a harder death than the complete death of a languishing life or the death of old age inasmuch as the leap is not so cruel from a painful life as from a sweet and flourishing life to a grievous and painful one."
Now, that is in a way an existential question about how one looks at one's finitude, if one has no intimations of the mortality in decline, and then there's the sort of further sort of social question of what the world is going to look like if, in addition to our cultivation of youth in which all kinds of people are not acting their age, they would now have no reason to act their age because they would, in fact, be in many decisive respects indistinguishable from what we all are when we're 30.
I'm not --
07-29-2004, 07:38 PM #4
DR. SWEENEY: Well, these mice still look old. They just don't look weak.
CHAIRMAN KASS: I believe we've got botox.
DR. SWEENEY: So much for Montaigne.
DR. FOSTER: Leon, I'm not sure that I agree with that. I think most people in medicine say that what they would like to do is to die suddenly with an intact mind, you know, healthy and with an intact mind. So that the lingering death that makes it maybe more wishful, I'm not sure that you'd be more afraid of this because most of us think that a sudden, you know, a Johnny Unitas death dying on the exercise thing is maybe the most blessed way one could go.
I don't know whether I buy into this --
CHAIRMAN KASS: I shouldn't be telling you that the Book of Common Prayer, if I'm not mistaken, asks that one should be delivered from sudden death for reasons which the gentleman at the end of the table --
DR. MAY: Sudden and unprovided for death.
CHAIRMAN KASS: Okay, but I wasn't introducing this as an objection to this, seriously.
DR. SWEENEY: No, I think you'd probably be outvoted by the population in general.
CHAIRMAN KASS: But it seems to me that this is one of those things which at first blush looks from a public health standpoint absolutely attractive, and yet it can't help but have all kinds of consequences for the perception of the life cycle and also for the relation amongst the generations even if us guys are a little more wrinkled than we were when we were 30.
That's not a moral objection that is going to stand, but I think one shouldn't underestimate the degree to which a change like this, if it became safe and if it became used would probably have profound consequences senility or no senility. The self-perception of oneself in an aging body is somehow part of our experience.
And if the body's aging which is mostly experienced by the fact that this ****ed equipment doesn't do what the will wants it to do and it used to do perfectly happy, I can't slide into second base anymore, though I still would like to do that; then it seems to me the feeling of a life would be different.
I'm not saying worse. It might be much better, but this is not a trivial matter about which you're speaking. It's not simply a public health matter. That was the point.
DR. SWEENEY: Yeah, perhaps I've sat out on this for a couple of reasons, personal, members of my family which were not so happily resigned to their loss of ambulation and basically lost their will to live because of it.
And also, I'm not going quite so happily myself in terms of reduced function. So --
CHAIRMAN KASS: Neither am I.
Bill, and then I think we'll stop.
DR. FOSTER: In my family, my boys pump iron, you know, and when I walk into the room the greeting is, "Hey, atrophied arms," and so I think I'm going to call Dr. Sweeney up and see if I can't get a little help for these insulting sons of mine.
CHAIRMAN KASS: For how many years do you want to fight them?
Bill, and then we'll stop.
DR. HURLBUT: Well, that's a hard comment to follow because what I was going to say was not just with a feeling of life changed from within a person, but the feeling of the relationships toward that life would change.
I mean, I remember watching my own father go through significant muscle atrophy and taking on a whole new relationship with him as I helped him move, and that was a meaningful part of the end of my relationship with my father.
And so I think in a way the larger question comes overarching here. Is the world in some way good either by the benevolent purposes of a creator or by the harmonious balance of a subtle evolutionary force or both, or is it just that one function was preserved because it helped the organism leave its genes in the next generation?
And it seems to me that's a crucial question because when I think about what you're talking about, the first thing that comes to my mind is IGF-1 does many, many things in the body, and you can't just go around tinkering with one thing and not damage a lot of things.
And that's the standard objection to these exaggerated projections mostly in popular magazines of the future of genetic engineering. People don't appreciate how complexly intertwined all of the genetic functions actually are.
We had pleiotropy and polygenic inheritance on the first level and then much more complex regulations and so forth. So if you change one thing, you're going to change many things.
But what you're suggesting raises a bit of an exception to that. You're suggesting that by targeting you can actually do a spatial and temporal modularization of a life reality, one that a more complex, overarching genetic system couldn't itself do.
So you're really suggesting there might be a way to bypass the multiple effects and possibly contain this. I mean, I know that you didn't overstate it.
DR. SWEENEY: Yeah.
DR. HURLBUT: You said the risks simply -- but this is the point. The question then becomes --
DR. SWEENEY: Yeah. That's the conceptual advantage of it.
DR. HURLBUT: The question then becomes: is the world right the way it was made or can we basically go in and alter the blueprint of the thing with a later revision of it?
That seems to me what the question comes down to. Is there some human benefit beyond an obvious therapeutic benefit to interfering in the way natural life unfolds?
DR. SWEENEY: Yeah, I guess it comes to a more philosophical issue. Was the aging process by design or simply neglect?
DR. HURLBUT: That's it.
DR. SWEENEY: I mean, is it a designed process or is it just the lack of forethought at a time in life when you're no longer contributing to the propagation of life?
And so we've sort of -- intellectually, from a scientific standpoint, I got at it that, well, this just wasn't thought through properly, and we can fix some of the things that could be tweaked a little bit to improve the long-term survival and performance of the tissue. Just addressing it strictly scientifically, not philosophically or morally, that's sort of the approach we're looking at, which is why the ability of gene transfer to give you modularization of the effect is so important to the type of thinking, because then you can think about affecting one type of tissue and not affecting the body as a whole.
DR. HURLBUT: Have you heard the saying Mother Nature always bats in the bottom of the ninth?
CHAIRMAN KASS: You should have the last word, I mean, if you'd like.
DR. SWEENEY: Well, again, I think it's whether or not Mother Nature designed the aging process or really just it's a fallout of not caring about the process.
CHAIRMAN KASS: Yes. I want to thank Dr. Sweeney for a very, very interesting and stimulating presentation.
I also want to thank you for your willingness to, not only on this occasion but on other occasions, alert people to the kinds of ethical and philosophical questions that your work raises and to lead us up to the edge of that and to entertain them and to do so in as thoughtful a way as you have.
So thank you very much. You've been a real service to the Council.
Thank you all. I will try to be in touch with you. I assume from some of the reports filtering back that some of you want to tell me things about yesterday. E-mails are, of course, welcome, phone calls too, and I'll be trying to get in touch with you in the meantime to talk about what's coming next.
Thanks for your participation. Safe trip home, and see you soon.
(Whereupon, at 12:11 p.m., the meeting was concluded.)
07-29-2004, 08:01 PM #5
Wow thats long, can you sum up the discussion??
07-30-2004, 05:24 PM #6
that was a great read thank you,
07-30-2004, 05:50 PM #7Anabolic Member
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07-30-2004, 11:25 PM #8Junior Member
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What an absolute wealth of knowledge! I cant thank you enough for posting this thread einstein. This has answered a lot of question I have had about gene doping, and it even touched a little bit on stem cell research!
I have to ask, if you were there and saw the slides. Did the mice with igf-1 over expression still die around the sime time as normal? Or where there lives extended past the 30 month mark?
I also thought it was really cool that none of the igf-1 was released into the bloodstream. This reduces a lot of riskes associated LR3, such as internal organ growth, or acromegaly, ect. Infact ist completly impressive how site specific gene dopeing, at the least in this setting actually is!
It would have been cool to see the slides of the muscle bound Rat/Mice!
Thanks again einstein!
07-31-2004, 12:52 AM #9Originally Posted by Bdy_Dysm
His studies are another big reason I suggest injecting IM. For some reason, even at very high expression levels within the muscle, the IGF-1 doesn't seem to leave the muscle efficiently, which would imply that it also won't enter the muscle efficiently from the bloodstream. it's an oddity and a bit unclear, as I know that there is substantial site-specific effects when I inject IM, but I also see generalized fatloss too. Clearly, subQ injections wouldn't be ideal though for the above reasons.
The lifespan was said to increase on average by 10%, which would likely be proven opposite with a larger sample number, as IGF-1 increases the rate of mitosis and therefore would contribute to telomere shortening faster, which is what determines the ability of a cell to continue dividing normally
07-31-2004, 09:48 AM #10Junior Member
Originally Posted by einstein1905
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Originally Posted by einstein1905
Last edited by Bdy_Dysm; 07-31-2004 at 02:54 PM.
07-31-2004, 11:49 AM #11
thanks for shareing bro,excellent info
07-31-2004, 08:33 PM #12New Member
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The Dr. Sweeney interview that Einstein posted was taken from the sixth meeting of the presidents council on BioEthics,,,,here is the link http://bioethics.gov/transcripts/sep02/session7.html
If you liked the Dr. Sweeney interview, the post below is a disscusion primarily on the Dr. Sweeney interview, given by the council members of the Presidents council of Bioethics.
Also, http://bioethics.gov/transcripts/jul02/session4.html is a good read if you like this sort of stuff,,,and yes,,,it's very long,,,,not too much technical talk about IGF,,,it's primarly more ethical questions on muscle enhancement and the future of sports.
Stronger, Long-Lasting Skeletal Muscles through Biotech? 1
THE LINK-- http://bioethics.gov/background/strong_muscles.html
Our muscles are essential to human life in a variety of ways. They are central components of physical strength and speed, attributes that are admired and celebrated in most human cultures. Our mobility depends on muscles, whether we use them to walk, or when we just use them to turn the wheel of a car or put our foot down on the accelerator or the brake. As a basic component of physical vigor they also play a role in human attractiveness. As such, muscle tone is a major contributor to the “sense of self” developed by each person. Although there are several different types of muscles in the human body, we will concentrate here on processes affecting skeletal muscles.
The strength and fitness of healthy muscles are largely a function of their exercise. In our youth, active use of our muscles in play and in sports strengthens and develops them. At puberty, production of estrogen and testosterone enhances these processes, so that the peak of human muscular development is usually between 20 and 30 years of age. Except for people whose daily work requires much physical exertion, maintaining peak muscular strength and endurance later in life requires regular exercise and fitness training. Some pursue this avidly, while others do not.
The strength and size of human muscles declines by about one-third between the ages of 30 and 80.2 Diminished capacity or loss of a previous ability to do a physical task is a common experience during human aging. The age-related loss of muscle size and strength has been named “sarcopenia”.3 In addition, there are a variety of diseases of muscle tissue (muscular dystrophies), many caused by specific genetic mutations.
We have an increased understanding of how many of the important genes in muscle cells function and are regulated.4 The parallel development of gene therapy techniques for efficient and controlled expression of genes is beginning to open up new possibilities for treating muscular dystrophies as well as maintaining “youthful” muscle size and strength
during the aging process. It is thus timely to begin discussions of the ends to which such increased understanding and power to modify should be put when it comes to human muscles.
As discussed in more detail below, biotechnological approaches to repair and strengthening of diseased and aging skeletal muscles have been demonstrated in experimental animals. The application of these approaches (once they are shown to be safe and effective) to treat human muscular dystrophies clearly falls within current understandings of appropriate therapy. A more difficult judgment is whether we should
extend the application of these approaches to a variety of other situations that are currently “beyond therapy”.
Muscles do not generate human strength and speed in isolation. Muscles need to be physically integrated with, and function harmoniously through their attachments to nerves, tendons, ligaments, and bones. While the focus in this paper is on the activity of muscle cells, we should remain alert to the possibility that biotechnological approaches that strengthen only muscles may lead to imbalances in the interactions with other components of the body, and subsequent malfunction.
Muscles in idealizations of male human form
Muscles play a prominent role in idealizations of male human form. A classical picture of excellence of the youthful male human form is Michelangelo’s sculpture of David, completed around 1504 (see Figure 1). Here the muscles are depicted as well-
Figure 1. Michelangelo's David
(ca. 1504) i http://www.sculpturegallery.com/sculpture/david.html
Figure 2. Arnold Schwarzenegger (ca. 1980) ii http://www.schwarzenegger.com/en/ath...sp?sec=athlete
proportioned but without much articulation of individual muscles. The strength and
power of David’s skeletal muscles shine through the marble, and leave us with a mental picture of the classical ideal of muscular development and proportion.
A more contemporary idealization of the male human form is the picture of the modern male body-building champion and actor, Arnold Schwarzenegger (Figure 2). Through specialized weight training and alleged use of anabolic steroids , his muscles (particularly the biceps) have become much larger than those pictured in the statue of David, and the different groups of skeletal muscles are individually articulated.
Although different in proportion and muscle articulation, both the classical and contemporary pictures testify to the importance of muscles in images of male strength and power. Large muscles were also supposed to help males attract females, a point that is still emphasized by males working out in gyms. “I am exercising and increasing the size of my muscles so that the chicks will notice me”.
Interestingly, female body-builders reportedly initially pursued the same path illustrated by the picture of Arnold Schwarzenegger. The result was female body-building champions with smaller but similarly individually developed and articulated skeletal muscles. More recently there has been an “aesthetic” reaction against the resulting female muscle “overdevelopment” and, commercially at least, the more popular and profitable activity today is women’s fitness competitions.
Genetic treatments are not the only biotechnological approach to increasing muscle size and strength. Anabolic steroids are among the most widely used chemical compounds that are used in combination with weight lifting to increase muscle size and strength. Examples of such compounds include methandrostenolone (Dianabo 1), Boldenone (Equip-gan), Stanazolol (Winstrol V) and Drostanolone (Masteron ). As information about their effects has diffused throughout American society, they are coming to be used more and more by professional and amateur athletes. Use of some of them is banned by anti-doping organizations. Many (including the ones listed above), are listed as available for sale on the Internet.
Cellular multiplication and differentiation in skeletal muscle
The major cell type present in skeletal muscle fibers is the multinucleated myotube. These fibers arise from the fusion of mononucleated myoblast cells with each other and with pre-existing myotubes. Myoblasts, in turn, are formed by differentiation of a particular stem cell found in muscle tissue, called a satellite cell.5
The multiplication and differentiation of satellite cells into myoblasts is regulated by several specific protein growth factors (primarily insulin -like growth factor 1 (IGF-1) and hepatocyte growth factor (HGF)) and also influenced by hormones such as growth hormone , testosterone and estrogen. Secretion of growth hormone by the pituitary acts on the liver to stimulate synthesis and release of IGF-1, which is released into the circulation (Figure 3). In muscle tissue, IGF-1 binds to specific receptors on the surface of satellite cells to stimulate cell multiplication, producing more satellite cells, and differentiation of satellite cells into myoblasts (see Figure 4).
Growth Hormone (GH) Secreted
SKELETAL MUSCLE growth stimulated
Figure 3. Hormone action and muscle growth stimulation
Importantly, a slightly different form of IGF-1 (mIGF-1) is also produced locally in muscle tissue in response to stretching the muscles (exercise). This form is thought to act the same way as circulating IGF-1 does in stimulating satellite cell multiplication and differentiation. However, because mIGF-1 is slightly different in chemical structure from IGF-1 produced in the liver, mIGF-1 apparently does not enter the circulation, so its effects can be restricted to promoting growth and repair of muscle tissue locally.
It is a common human experience that muscle size and strength can be increased by exercise. The number of muscle fibers increases as a consequence of exercise-induced stimulation of the multiplication and differentiation of muscle stem cells. Exercise both transiently damages muscles and causes them to increase in size and strength.
While exercise was previously the only way to do this, biotechnological research and
Figure 4. Schematic diagram of some important processes in skeletal muscle fiber growth and repair.
development have introduced new possibilities. The genes for animal and human IGF-1 have been cloned and their DNA sequences determined. Gene expression vectors have been developed that permit the regulated production of IGF-1 proteins (both the liver and muscle forms) for investigation. So IGF-1 genes can be introduced into cells and experimental animals to determine the effect of enhanced IGF-1 (and/or mIGF-1) production on muscle size and strength.
Loss of muscle size and strength on aging: sarcopenia
With aging, we become more sedentary and use our muscles less. With aging the production of growth hormone and circulating IGF-1 also decreases. There is thus less IGF-1 available to keep the muscles large, and they become smaller and weaker. In addition, aged muscle cells are apparently less responsive to the action of IGF-1 and mIGF-1 6 so that the impact of even vigorous exercise on muscle size and strength diminishes with age. Figure 5 graphically illustrates the appearance of leg muscles as they become smaller and weaker with age (sarcopenia).
As we age, several things change that predispose to the development of sarcopenia. We either reduce the output of, and/or become more resistant to, anabolic stimuli to muscle such as central nervous system input, growth hormone, estrogen, testosterone, dietary protein, physical activity and insulin action. The loss of alpha-motor neuron input to muscle that occurs with age7 is believed to be a critical factor8. Nerve cell-muscle cell connections are critical to maintaining muscle mass and strength.
A loss of muscle size and strength in a significant problem for older persons. While not painful or directly debilitating, sarcopenia is associated with an increased tendency to fall and break bones. Such falls and broken bones are major causes of morbidity among the elderly.
This image is from the informative Internet site www.sarcopenia.com.
Figure 5 – Illustrating progressive age-related loss of muscle tissue (sarcopenia) iii
Selective stimulation of skeletal muscle growth in experimental animals.
Local injection of regulated exogenous “muscle-specific” IGF-1 Gene
Recombinant viruses, engineered to express a specific foreign gene, are frequently used to stimulate the production of functionally effective amounts of the foreign protein to treat disease. Recombinant viruses created from genetically engineered human
Adenovirus-associated Virus (AAV) have proved to be efficient delivery systems of foreign genes into muscle cells. As AAV is a small virus, only small foreign genes can be used effectively with this virus. Fortunately, the DNA sequence encoding IGF-1 is small enough to function well in AAV-based recombinant viruses.
In experiments described by Barton-Davis and coworkers 9, AAV recombinant viruses containing a rat IGF-1 gene were injected into the anterior compartment of the rear legs of mice containing the extensor digitorum longus (EDL) muscle. The resulting increased IGF-1 production promoted an average increase of about 15% in EDL muscle mass and strength in young adult mice. Strikingly, such injections led to a 27% increase in the strength of the EDL muscles of 24-month (old) mice, thus substantially reducing the decrease in EDL muscle size and strength observed in untreated old mice.
In this study, approximately 1 x 1010 recombinant AAV particles in 100 µliters of fluid were injected into a single small muscle compartment of mice. If such treatments were eventually to be applied to humans, large amounts of recombinant AAV containing the human IGF-1 DNA sequence would be required. Assuming such future treatments were shown to be safe and effective, producing sufficient recombinant AAV to treat millions of dystrophic and aging humans would remain a substantial logistical challenge. However, there may be ways around this logistical problem involving the production and transplantation of human muscle stem cells engineered to produce more IGF-1 (see below).
IGF-1 transgenic mice
The ability to create transgenic mice, in which an appropriately regulated foreign gene is expressed throughout embryonic and adult life, offers another way to assess the biological role(s) of the transgene product. Musaro et al10 introduced a rat mIGF-1 transgene into early stage mouse embryos, where it became integrated with mouse chromosomal DNA. The resulting transgenic mice produce substantial amounts of rat mIGF-1, in addition their production of mouse IGF-1 and mIGF-1.
In these transgenic mice, the rat mIGF-1 transgene was connected to gene expression regulatory elements that restricted production of the rat mIGF-1 protein to muscle tissues containing primarily fast twitch fibers. Embryonic development of these transgenic mice proceeded normally. However, as early as 10 days after birth, enlargement of skeletal muscles where rat mIGF-1 protein was being produced was observed in the transgenic animals compared to the non-transgenic control mice.
Moreover, the skeletal muscle enlargement persisted as the transgenic mice aged.
Muscle size and strength were maximal around six months in unmodified (wild type) mice and decreased as expected by 20 months of age. In contrast, at 20 months the size and strength of skeletal muscle in the rat mIGF-1 transgenic mice remained at essentially the same as at six months.
In previous studies of this type, the IGF-1 transgene was not connected to gene expression regulatory elements that restricted production of mIGF-1 to muscle tissue.
This led to overproduction of IGF-1 in the circulation, and eventually to pathological enlargement of the heart muscle. The growing understanding of muscle physiology at the molecular level coupled with sophisticated genetic engineering has made it possible to enlarge skeletal muscles selectively, without damaging heart muscles in the process.
These and other experimental results stimulate thought about possible extensions of these approaches to humans. Similar procedures might be useful treatments for various diseases of muscle tissue, and well as a possible use in older persons to counteract sarcopenia. However, each of the procedures described above has technical/logistic problems that would need to be overcome before any treatment could be applied on a large scale.
Could these biotechnological approaches be applied to human muscles?
Could the mIGF-1 gene procedures that increase skeletal muscle size and strength in young and old experimental animals be adapted for use in humans? Based on our current understanding, at least three different approaches could be considered. First, one might develop recombinant AAV-based virus vectors containing the human mIGF-1 gene under the control of appropriate regulatory elements that would limit its expression to muscle cells near the site of injection. Alternatively, one might introduce an appropriately regulated mIGF-1 gene into human embryos, as was done in the experiments with mice. Finally, a combined approach might be developed in which one first isolated human muscle stem (satellite) cells and expanded them in vitro, next introduced an appropriately regulated human mIGF-1 gene into those cells in vitro, and finally transplanted the genetically modified satellite cells back into the muscles of the person being treated.
The first approach would be similar to other human gene therapy projects. The appropriately regulated human mIGF-1 gene would be combined with a vector capable of efficient delivery to muscle cells, perhaps AAV. This material could be produced in large volumes, carefully characterized by tests in experimental animals, stored frozen and used as needed. While the logistics of producing the large amounts of recombinant AAV that would be required for treatment of thousands or millions of patients are daunting, in principle this would be possible. The advantages of this approach are 1) that it would develop and use a single, well-characterized biological agent; 2) that treatment could be started very slowly by introducing the recombinant mIGF-1 gene-containing AAV into one muscle at a time and evaluating its effects; 3) that treatment could be stopped immediately if untoward side effects developed. Disadvantages include 1) the possibility that a large number of injections would be necessary to treat each of the large number of human skeletal muscles; 2) the possibility that this would not be an effective treatment for humans who had antibodies to AAV as a consequence of a previous infection.
The second approach is a radical proposal, as it envisions treatment of blastocyst stage human embryos in vitro with a genetic procedure that was intended to change the early development of skeletal muscle size and strength and reduce the rate of loss later in life. This approach shares some advantages with the first approach in that a single biological agent could be prepared and characterized that could treat all embryos; 2) that only a single treatment early in embryonic development would be needed, instead of multiple injections into different muscles. The major disadvantages of this approach are the difficult ethical questions it would raise.
The third approach depends upon the ability to isolate human muscle stem (satellite) cells and expand them in vitro. The isolated human muscle stem cells would then have their mIGF-1 production genetically modified by introducing an appropriately regulated exogenous mIGF-1 gene copy. In theory, this could produce modified muscle stem cells that multiplied continuously in vitro to produce larger numbers of cells, and that differentiated appropriately in vitro. In this case, genetically modified satellite cells would be injected into the aging skeletal muscles. The advantages of this approach include 1) that it would develop and use a single, well-characterized biological agent to modify the muscle stem cells in vitro and 2) the dose of modified stem cells could be varied as necessary to optimize treatment of individual skeletal muscles. The disadvantages include the possibility that a separate preparation of muscle stem cells from each patient to be treated would have to be made in order to get around the immune rejection problem.
Each of these approaches has advantages and disadvantages. Developing any one of them would take a lot of time and money. Before genetic treatments to increase muscle size and strength are tried in humans, the US Food and Drug Administration would require demonstrations that the proposed treatment is safe and effective. This will ensure regulatory oversight of any initial experiments along these lines with humans.
The initial steps in applying to normal humans the kinds of genetic approaches to increasing muscle size and strength described here will likely be performed in the course of using these procedures to treat human muscle diseases. Clinical trials of regulated mIGF-1 gene delivery as a treatment for specific forms of muscular dystrophy may begin within the next several years (Sweeney, personal communication). Data on route of administration, optimal dose and possible side effects will be obtained from these clinical trials. If efficacy is demonstrated and side effects are small, one can imagine that many people, young as well as old, might well be interested in receiving genetic muscle treatments to enhance muscle size and strength. Developing a product for which the eventual potential market is 100% of the human population will be hard to resist.
Some human contexts for future genetic muscle treatments
What would be the human significance of genetic muscle treatments becoming safe, inexpensive and thus potentially widespread in the future? How would it change the physical (and mental) experience of middle and old age? Preventing the decline of skeletal muscle size and strength in older persons would probably decrease the number of their falls and fractures, but would it also decrease the apprehensiveness and growing timidity that frequently accompany old age? Would such application come to blur the distinction between being “young” and being “old”? Would there be any changes in relations between the generations if the young ceased to be physically superior to the old?
How might such future genetic muscle enhancement be used by persons between the ages of say 20 and 50? Given the popularity of body-building and fitness today, one could imagine its use to enhance those activities, both in competitive and non-competitive settings. The commercial and competitive pressures to use genetic muscle treatments to build up, maintain and repair the muscles of competitive professional athletes in all sports would be very strong. Are not pressures to build muscles (even using anabolic steroids) already felt by student athletes in colleges and high schools? Since athletic competition extends down to youth soccer and Little League baseball, would there any place to draw a line against using genetic muscle treatments?
What would be the responsibilities of parents toward their children’s muscle development in a society where genetic muscle treatments were safe, inexpensive and widespread? Should parents allow their children’s muscles to develop “naturally” through age 20? What should they do when daughter Jenny’s soccer coach tells them she would be a stronger player if they got her genetic muscle treatments, or that she won’t make the team unless she gets treated? Would untreated children become stigmatized in a society where many others had genetic muscle treatments?
Should these biotechnological approaches be applied to aged human muscles?
“Sarcopenia” is currently a descriptive term for the loss of muscle size and strength that accompanies aging. It is not yet the name of a human disease. We will have to come to some societal judgment about whether sarcopenia is a disease, and therefore whether biotechnological approaches to treating it are appropriately termed therapies.
Within American society, there is probably a diversity of views about sarcopenia. One view would assert that loss of muscle strength with age is part of the natural human condition, part of the natural trajectory of human life. From this starting point, attempts to delay this process are “unnatural” and therefore suspect. At least, it would be argued, there is no moral requirement that modern medicine move heaven and earth to fix this problem, particularly by expensive, novel biotechnological means.
An alternative view would point to the fact that loss of muscle strength on aging predisposes to falls and broken bones, major sources of morbidity in the elderly. If we could prevent or delay such loss of muscle strength and thereby decrease the frequency of broken bones in the elderly, would not both individuals and society benefit?
Having agreed that sarcopenia puts a name on an important social problem, doesn’t mean that we must be committed to muscle gene injections as the solution. “Old-fashioned” approaches such as diet and exercise are effective at slowing the loss of muscle size and strength during aging. Would it be a good use of brainpower and resources to develop genetic approaches to treating sarcopenia, if in the end all it does is substitute for regular gym visits by older persons for resistance strength training?
Genetic muscle treatments are being evaluated as possible therapies for various dystrophic conditions of skeletal muscle. However, as this paper points out, once such a technology is developed and applied within the medical sphere, there will be substantial pressures to use it in a variety of settings that are currently “beyond therapy”. Genetic muscle treatments that go “beyond therapy” are another example of an emerging bioethical dilemma…should we apply our increasing knowledge of human biology to give future generations of humans biological capabilities that past generations never had?
07-31-2004, 10:37 PM #13
Have been looking into this extensively, and after reading this, seems all the more promising. Helped me make the decision to finally break down and try it...
Now for the legalities of this compound...do I have to go hunting for secret sources and be wary of sites offering it and thier legitimacy, or is this in a "gray area" where it could concievably be a safe buy from a legit online site. Nobody I know or deal with carries this (LONG R3 I mean) and not something I'm looking to f*** around with from someone I don't know.
F*** it...I'm jumpin the fence at can't post sites and gettin me some Somatokine
07-31-2004, 11:24 PM #14Originally Posted by Justin_Case
07-31-2004, 11:27 PM #15Junior Member
Originally Posted by John99Originally Posted by John99
- Join Date
- Mar 2004
This (and I include more than just the above) certanly raises a number of moral and ethical questions. That there are an infinate number of possible answers for. All of with is measured only by ones personal views, and or opinoins. Wich I'm affraid does not hold a right or wrong answer for!
I think it would be safe to add "or Gene doping", to the phrase "It is not safe to discuss politics, and religion"!
Its interesting to see where all this leads!
Thank for your contribution to this board. It is much appreciated!
07-31-2004, 11:32 PM #16
What a great thread einy...I need to go back and read it again when I'm not so tired.
07-31-2004, 11:39 PM #17Junior Member
- Join Date
- Mar 2004
[QUOTE=Da_Bull]......I need to go back and read it again when I'm not so tired.
I second that!
I could'nt of possibly retained everything in this thread reading it only once!
Last edited by Bdy_Dysm; 07-31-2004 at 11:41 PM.
08-01-2004, 02:43 PM #18Originally Posted by Justin_Case
08-24-2004, 07:03 PM #19New Member
- Join Date
- Jul 2004
Anyone find the study done by Evans on the marathon mice,,,,I haven't found it, so I'm guessing it hasn't been published yet. Sounds similiar to sweeney and other's work, but I haven't gotten any details.
03-26-2005, 12:59 PM #20
im curious , do any of these " IGF-1 LR3. IGF-1 " help with tendon , ligament , cartlege regeneration ?
03-26-2005, 01:10 PM #21Anabolic Member
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- Jun 2004
Bro, why are you bringing up this old thread? No need to , start a new one. Not giving attention to a banned individual who was banned rightfully so.
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