Thread: suspended in Acetic acid?
02-27-2005, 09:08 AM #1
suspended in Acetic acid?
should i treat IGF-1 LR3 thats suspended in Acetic acid the same as if it was in BA? IE: How long its good for and how it will be in the freezer? Any info would help.
02-27-2005, 02:07 PM #2
If its suspended with AA do not keep it in the freezer like you do with BA...
02-27-2005, 03:56 PM #3
Oh thanks... then keep it in the fridge? How long will it last in the fridge?
Originally Posted by Mr. Sparkle
02-27-2005, 04:14 PM #4
3-4 weeks is the best guess. Some companies that sell it say 3 months...
Originally Posted by juicehoe
02-28-2005, 02:56 AM #5Associate Member
- Join Date
- Apr 2004
i got this from johnny B.in acetic acid reconstituted and kept in fridge will keep for 2 years:-
Recombinant analog of human insulin growth factor-I
CATALOG NO. 85580
Long™R3IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) that has been specifically engineered for the enhancement of cell culture performance. Long™R3IGF-I is more biologically potent in vitro than either insulin or native IGF-I and has been documented to
significantly increase recombinant protein production. It is ideal for both research and large-scale culture systems utilizing serum-free or low-level serum applications. Long™R3IGF-I is effective in any cell that contains type I IGF receptors, including most Chinese Hamster Ovary (CHO) lines, hybridomas and fibroblasts. Long™R3IGF-I is produced in a patented E. coli expression system without the use of animal-derived components.
This product is for research/laboratory use. THIS PRODUCT
IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.
Long™R3IGF-I in lyophilized form should be stored at 2 to 8 C and is stable for at least 3 years. Liquid solutions of Long™R3IGF-I (prepared as described below) can be stored at 2 to 8 C and are stable for at least 2 years.
1. Long™R3IGF-I is supplied lyophilized in an atmosphere of nitrogen at a slight vacuum (-25 kPa). Remove the metal cap from the glass vial and introduce an air filled syringe
through the septum to equalize the pressure.
2. Add sufficient 10 mM HCl or 100 mM acetic acid solution to the vial to achieve a peptide concentration of at least 0.1 mg/mL. Concentrations of 1 mg/mL or more are recommended.
3. Mix the solution thoroughly to insure the peptide is completely dissolved.
4. Filtration of a Long™R3IGF-I solution or a culture medium after the addition of Long™R3IGF-I should be performed using a low protein-binding membrane such as Polyvinyl Idene Flouride (PVDF), Poly Ethylene Sulfite (PES) or Cellulose Acetate (CA).
Methods for Use
Once Long™R3IGF-I has been reconstituted, it can then be added directly to cell culture medium. A titration of Long™R3IGF-I should be performed for each different application as the optimum concentration may vary depending upon the cell type used and other components present in the medium. The recommended final concentration range of Long™R3IGF-I is 10 to 50 µg/L (or ng/mL). Because Long™R3IGF-I and insulin may compete for the same cell receptors, the effectiveness of Long™R3IGF-I will be masked if it is added in conjunction with commonly employed concentrations of insulin (1 to 10 mg/L). However, inclusion of physiological levels of insulin (5 to 10 µg/L) in cell culture medium containing the recommended levels of
Long™R3IGF-I results in beneficial synergistic effects in certain applications.
9110 ± 2 daltons (Mass spectroscopy)
< 0.1 EU/µg
ED50 < 10 ng/mL (Bioassay assessing the stimulation of
protein synthesis in L6 myoblasts)
02-28-2005, 08:49 AM #6
Thanks great read toc67guru
02-28-2005, 12:53 PM #7Associate Member
- Join Date
- Apr 2004
its not mine unfortuneately i got it from johnny B as i asked the same question.
for lr3 suspended in benzyl alcohol it was best to keep it in the refridgerator and it was active for only months.
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