How to make oil sterile?

[SilverBack_69]

Hey guys!

I want to buy some cottonseed oil from AR to cut my prop with. Can anyone tell me if this oil is already sterile? If it is not what do I need from AR to make sterile oil? Do I just get an whatman syringe filter and a sealed vial or an un-sealed vial? If I just get my own grapeseed oil from a health food store will baking for 45min at 400 deg F work. I just want to be safe and unsterile oil doesn't sound safe.

[alphadog]

BUMP i would like to know this as well

[peachfuzz]

its usp grade so that takes some worry out of it but i would place it into a sealed sterile vial, vent it with a pin and bake at 250-275 for 30 minutes.

the BA in the prop should take care of anything else.

dont take my word for the gospel tho. bump for more answers

also try the lab section

[alphadog]

Bump

[legobricks]

Run it through a whatman filter into a sterile vial and you should be fine.

If your that worried add some BA to the oil....although im not too sure on the % needed to add right now.... check out the lab section.

[alphadog]

run it through a whatman filter into a sterile vial and you should be fine.

If your that worried add some ba to the oil....although im not too sure on the % needed to add right now.... Check out the lab section.

thanks bud

[diesel405]

You can buy oil from a grocery store, i use grapeseed for prop. Their is no need to bake anything, in fact its not a good idea, unless your in a sterile environment. Run the oil thru a .22 micron filter and it will be sterile....

[alphadog]

You can buy oil from a grocery store, i use grapeseed for prop. Their is no need to bake anything, in fact its not a good idea, unless your in a sterile environment. Run the oil thru a .22 micron filter and it will be sterile....

grapeseed oil that they sell in FAIRWAY MARKET through a whatman .22 filter into a sterile vial and im legit to poke????

[diesel405]

Yes, no bacteria can fit thru a .22 micron filter. If you use a .45 they can get thru. I no this 100%, my wife is a scientist.

[ninesecz]

Hey guys!

I want to buy some cottonseed oil from AR to cut my prop with. Can anyone tell me if this oil is already sterile? If it is not what do I need from AR to make sterile oil? Do I just get an whatman syringe filter and a sealed vial or an un-sealed vial? If I just get my own grapeseed oil from a health food store will baking for 45min at 400 deg F work. I just want to be safe and unsterile oil doesn't sound safe.

Put tho oil on a cycle of heavy test and deca.... That should make it pretty sterile LOL!!

[alphadog]

Yes, no bacteria can fit thru a .22 micron filter. If you use a .45 they can get thru. I no this 100%, my wife is a scientist.

Filtered into a sterile vial, the oil will stay sterile??? or for how long a time period??

[diesel405]

Without adding benzyl alcohol im not sure how long it will stay sterile. All gear has 2% ba in it and it lasts for years. She's not home now,lol, but i can ask her tonight and i'll pm you.

[musclehead1]

there was a experiment i read about heating oil to kill bacteria, but i can't remember where it is. They said that oil that was baked at 160 degrees Celuis for one hour and it killed all bacteria. They tested the oil for bacteria growth right after heating it, one day after and one week after (and at another time but i can't remember when) no growth was present. You might want to check to see if bacteria can get through a .22 whatman, because i believe bacteria spores can get through. Best thing to do would be to get USP grade oil, or heat (like stated above), filter through .22 whatman filter into a sterile vial, and add the correct amount of ba.

[diesel405]

Yes, heat over a specified amount of time can kill certain bacteria. Unfortunately, the problem lies with where you are going to heat the oil. Unless you have an autoclave accessible, once you remove the oil from the oven, it is now exposed to an unsterile environment. Heating it as they did may have reduced bioburden but I wouldn't say it was sterile. Filtering it through a 0.22 micron filter within a closed system (sterile vial) would guarantee it sterile. As for how long it would remain sterile.................you should be good to go for awhile as long as that vial is never opened to the environment. Not sure on an actual timeframe as you would need stability data to support that. <---- thats the wife, not me....lol

[musclehead1]

Yes, heat over a specified amount of time can kill certain bacteria. Unfortunately, the problem lies with where you are going to heat the oil. Unless you have an autoclave accessible, once you remove the oil from the oven, it is now exposed to an unsterile environment. Heating it as they did may have reduced bioburden but I wouldn't say it was sterile. Filtering it through a 0.22 micron filter within a closed system (sterile vial) would guarantee it sterile. As for how long it would remain sterile.................you should be good to go for awhile as long as that vial is never opened to the environment. Not sure on an actual timeframe as you would need stability data to support that. <---- thats the wife, not me....lol

Correct me if im wrong, but I think you are assuming that I mean to heat the oil in an open dish? But I mean to inject it into a closed sterile vial--> heat--> filter (.22 whatman) into a different sterile vial---> add ba. I'd probably use this method if i couldn't find any sterile oil.
I have also heard of guys taking oil bought from the supermarket and just mixing it with their gear, no heating or filtering just straight out of the bottle. They claim the ba in the gear makes it sterile. But i wouldn't recommend that.

[diesel405]

Would a sealed vial even take that kinda heat? That is just a rubber stopper. Then your injecting it into a vial, baking it, sucking it outta the vial, filtering it and injecting it into another vial. Thats really not necessary bro. Your not only making things harder, but every time you go in and out of a vial your risking contamination. Remember one thing, nothing has to be sterile b4 it goes thru the filter. What comes out of the .22 micron filter is sterile. Also, i and and many others do use oil from the grocery store, filter it and its fine. Also ba doesn't sterilize, it prevents the growth of bacteria. Big difference. What i suggest to you is head on over to the lab and make a post about filtering. You will hear a couple different suggestions, but my boy SWOLE25 and a few other vets will head you in the right direction. Believe me, i only speak on personal experiences,things i have done. I wouldnt tell you something i was unsure about. And i trust my girls degree's more than i trust something i have read about on any web site. Good luck bro.......