Do whatman filters work in reverse??

**newyboy** · 02-28-2010, 09:33 PM

Hey guys, just after some advice.. I got a few vials of contaminated gear, iam halfway thru a cycle and i cant get any sterile vials at this momnet to transfer them into and i need too inject today.. my question is can i put a whatman on a syringe and pull the oil up through the whatman into the syringe and preload a few of them until i can get some sterile vials??
Or do they only work in one direction??
Thanks guys!

**AnonEagle** · 02-28-2010, 09:55 PM

Hm, sorry don't know the answer to your question. But, if not, you could bake your gear to kill the contaminants.

**Bonaparte** · 02-28-2010, 10:02 PM

Due to the filter design, they will not work right if used in reverse (nor would you be able to create enough of a vacuum).

**newyboy** · 02-28-2010, 10:07 PM

Buy baking do u mean put in a normal kitchen oven , while still in the rubber top vials?? If so how long do i do it for and what heat?? Does it work as good as whaman??

**spywizard** · 02-28-2010, 10:38 PM

Originally Posted by AnonEagle

Hm, sorry don't know the answer to your question. But, if not, you could bake your gear to kill the contaminants.

no.. you cannot

use the filter, otherwise start pct if you can't get a sterile vial..

have you seen an image of an abscess?? just say no

**kaboom** · 03-01-2010, 12:33 AM

Just curious but how do you that they are contaminated?

**AnonEagle** · 03-01-2010, 01:28 AM

Originally Posted by spywizard

no.. you cannot

use the filter, otherwise start pct if you can't get a sterile vial..

have you seen an image of an abscess?? just say no

are you saying no you cannot bake gear? i've read it from countless sources on the topics of sterilization and homebrewing. interested in hearing why you say baking is not possible.

most people say 20-30 minutes at ~250degrees, maybe repeated multiple times, and poke a sterile needle in the rubber to let the expanding air vent. this temp will not melt the rubber.

although, I just read this, which makes a lot of sense, and would still be completely effective.

Originally Posted by realgains@EF

FYI
I friend of mine is a pathologist in the hospital that I work in and yes he has used gear.
We got to talking about sterilizing steriods , especailly home brewed tren . He says that baking tren or any steroid at 250 degrees F is not a good thing at all. He says that it will denature some of the hormone. He went on to recommend pasturization at between 150 to 170 degrees F for 30 minutes. Buy a candy thermometer and heat water to this temperature range then put the vials in the water. Before doing this be sure to withdraw air from the vial to allow for safe temperature expansion of the remaining air.

Interestingly Bill Roberts says the exact same thing.

...

Ya leaving a pin in the vail is a great idea but be sure to cover the majority of the vial with the hot water.

BIll Roberts says that even a few minutes at 170 degree F will kill the bacteria but that the oil will need time to reach this temp thus the 30 minutes. This is what is done to kill the bugs in milk and milk can have some pretty nasty bacteria in it.

My buddy says that this temp will not destroy heat spores but either will 250 degree. Luckily spores are few and far between

My pathologist friend added that a .2 micron filter will also do the trick. I'll ask him if this will trap spores

**newyboy** · 03-01-2010, 02:49 AM

Thanks guys, i know gear in contAM because yes i got an absuss from it, and after i filtered it , it was fine!
I Need too shoot today, so i think ill pull the backs off some 3ml syringes and preload them until i can get some sterile vials...

**taiwanguy** · 03-01-2010, 03:26 AM

You don't have any old vials that are empty? If you do (assuming they were sterile to begin with) then just filter in to those. I know I've got a bunch of empty vials I keep with my other ones that have oil in them.