Why peptides you use should be from a recombinant DNA source and not a (*MUST READ*)

[Juced_porkchop]

Why peptides you use should be from a recombinant DNA source and not a chemically synthesized source

I want to bring something up about peptides and peptide quality. I see many people going the cheap route but there is a reason that they may be simply wasting their money. That is why I wanted to start a thread about an issue I think is going on related to efficacy of chemically synthesized IGF and most other peptides vs. recombinantly made ones.

There are many threads on here about IGF-1 and other peps giving good gains and I feel some of it may be BS or the person is having a "placebo affect", while others swear peptides are a complete scam and do nothing at all! (not true)... There might be a reason your igf1 cycle didn't give you jack shit?

Why?

I believe most are purchasing IGF-1 (for an example of a popular peptide being used) made by cheap peptide synthesis companies. However, chemical synthesis of IGF-1 lacks the correct disulfide bonds that cause the proper folding and function in the peptide chain. IGF-1 and its many analogs require 3 disulfide bonds to generate the correct folded form of the protein and this can only be accomplished in the body as naturally produced, or in E.coli bacteria as a recombinant source which is similar to how HGH is made (recombinantly).

Insulin is also very similar to the structure of IGF-1 and it also is inactive and/or unstable if synthesized chemically, it has to be made recombinantly or extracted from a biologic source (they used to extract insulin from bovine and HGH from cadavers).
Made with "recombinant DNA" means it is made in bacteria which have data input into it and "fermented" create enzymes that make these intramolecular disulfide bonds so that the protein folds into the right configuration and has the biological function it is supposed to have, having the proper amino chain is NOT enough and it is NOT that simple!

If you look into human clinical trials or current clinical treatment of patients, they now use some of these peptides as part of a therapy protocol and they use recombinant DNA made peptides, not chemically synthesized ones and for good reason.

Here is just one of many studies on how important the correct bonds are, not just the structure>
Role of native disulfide bonds in the structure... [Biochemistry. 1993] - PubMed - NCBI > Role of native disulfide bonds in the structure... [Biochemistry. 1993] - PubMed - NCBI


There are many cheap peptide sellers popping out like CRAZY the last couple years with ridiculously low prices that just doesn't make any sense. So I say if it seems too good to be true, then it probably is!
There is a reason some places are so cheap while others can cost more than 2X or 3X the amount of the cheaper places.

You usually get what you pay for so PLEASE do your research first!

Just because it's pricy doesn't automatically mean its top quality, you still need to research the company.
But if it is really cheap then I would just avoid it because the chances of it being properly made are minimal in this case. IMO

There is a flood of shotty quality peptides coming from china, BE SAFE AND AVOID!

What you want is the more expensive recombinant DNA made peptides! NOT the cheaply made, unstable and largely biologically inactive chemically synthesized peptides!

Many don't even have a clue about all this and that is why I am posting this thread.

Hope it helps someone!


Cheers

[jimmyinkedup]

Damn good post Juced!

[Spawned]

That does make a lot of sense. Thank you for the insight.

[Juced_porkchop]

That does make a lot of sense. Thank you for the insight.

No prob!
Happy if it helps even one person! :-)

There is just soo much that comes into peptides and there quality/biological activity, not as simple as just correct amino chain.

[mockery]

No prob!
Happy if it helps even one person! :-)

There is just soo much that comes into peptides and there quality/biological activity, not as simple as just correct amino chain.

My dr is pro peps and said this very thing, she was at peptide convention two months ago and said this is something they touched based on for some time.

[kelkel]

Outstanding JP. Keep this stuff coming.

[DanB]

Nice post very imformative, thanks

[Juced_porkchop]

My dr is pro peps and said this very thing, she was at peptide convention two months ago and said this is something they touched based on for some time.

Outstanding JP. Keep this stuff coming.

Nice post very imformative, thanks

Thanks guys!

I sure as heck plan to keep em coming as I have time to. I love to learn about this stuff and love even more to help people with what I learn or exp on my own with. Sure sometimes I am wrong about something or maybe slightly off ( like for example how cjc1293 AND 1295 BOTH HAVE DAC!!! but one is quicker acting! some places will say cjc1293 W/O Dac but either it is a lie OR it should be called a Mod-GRF (vague term BTW which can include more than one compound) or sermorelin/ Mod-GRF 1-29, NOT cjc. atleast this is what 2 chemists told me AND i have read about when I investigated) anyway, but that is great when I am off on something, because then we can all learn! I am happy to say I am not usually wrong though ;-) haha



I love this forum and the more time I have from work the more ill post. I am a MOD at a few forums, but this is one I just really like. I haven't posted as much as I have been reading. but there is just something about this forum I LOVE, that is not like many others, so continue I WILL! :-D

[austinite]

Outstanding post Juced. No surprise coming from you. I always refer to you and jimmy's posts with respect to the topic especially.

Thanks for the info brother!

[Juced_porkchop]

Outstanding post Juced. No surprise coming from you. I always refer to you and jimmy's posts with respect to the topic especially.

Thanks for the info brother!

THANKS MAN! : )


Many have no idea its not as simple as asking for a pep by name...

[RigPig]

Damn...how the hell does a guy tell if it's Recombinant or chemically Synthesized?
My source assures his Peps are from a company in Ont...Other than trusting him on this is there another way?

[chris_columbia]

Is maxim peptides gtg? That's where I've gotten my clen and AI from.

[SpanishStallion]

While this discussion is interesting its only applicable to peptides that can be manufactured by both chemical and recombinant technology. IGF-1 is one case but you wouldn't see anyone producing GHRP's or modified GHRH by fermentation. Basically most used peptides like CJC with DAC, MOD GRF, HGH FRAG and GHRP's are all manufactured by chemical synthesis and have a high bio-availability. I haven't studied the bio-availability of chemical sequenced IGF-1 (or the lack of it) but until I see a comparative study the article is based on circumstantial evidence.

[gold43]

I am Pro Peptides! I would like to see this research/convo continue! Great post JP!!! As well as a ******* point above from SS.

[APIs]

All well & good, but no one can confirm what they're actually getting in the first place or where it's really being made. The whole peptide industry is smoke & mirrors at best...

[XxAndreaxX]

I must say, I used a 60$ 1mg IGF-1 LR3 vial and it seemed to be G2G. I did not gain any monster size in 4 weeks of use off course, but sides were g2g, the typical sides described for IGF-1 or insulin . So either the IGF-1 was real, or it was plain long acting insulin. My melanotan was also very cheap and G2G, I got a deep dark tan, and crazy sides.

[Juced_porkchop]

Damn...how the hell does a guy tell if it's Recombinant or chemically Synthesized?
My source assures his Peps are from a company in Ont...Other than trusting him on this is there another way?

Assuring what location they are made in does not tell you how its made... you could ask but unless you got some advanced facilities at your disposal... trust and track history sadly what you go by on top of what they say..

Is maxim peptides gtg? That's where I've gotten my clen and AI from.

I have seen them at many place and judging from what I have read and some of price I would lean towards they are just a reseller of Chinese made peps and who knows on quality... but my main concern with your question is that Clen and AI's are not peptides... so this thread doesn't apply in the same way.

While this discussion is interesting its only applicable to peptides that can be manufactured by both chemical and recombinant technology. IGF-1 is one case but you wouldn't see anyone producing GHRP's or modified GHRH by fermentation. Basically most used peptides like CJC with DAC, MOD GRF, HGH FRAG and GHRP's are all manufactured by chemical synthesis and have a high bio-availability. I haven't studied the bio-availability of chemical sequenced IGF-1 (or the lack of it) but until I see a comparative study the article is based on circumstantial evidence.

while this is tru some can be made both ways. as IGF1 could... it doesnt mean its functional as it would be made by recombinant dna. some would seem to have full action and others would be sub-par IMO.

I am Pro Peptides! I would like to see this research/convo continue! Great post JP!!! As well as a ******* point above from SS.

Thanks!! : )

All well & good, but no one can confirm what they're actually getting in the first place or where it's really being made. The whole peptide industry is smoke & mirrors at best...

100% agree! why I avoid all these new places that pop up or been around for only a couple years... go with a long track recired and places that seem to know what the heck they are talking about... trust me I got in arguments with some pep sellers that would not answer my basic questions or gave me "bro logic" answers they clearly just read online and did not deeply research... its a mess in the pep / RC game for sure!

I must say, I used a 60$ 1mg IGF-1 LR3 vial and it seemed to be G2G. I did not gain any monster size in 4 weeks of use off course, but sides were g2g, the typical sides described for IGF-1 or insulin. So either the IGF-1 was real, or it was plain long acting insulin. My melanotan was also very cheap and G2G, I got a deep dark tan, and crazy sides.

what do you mean sides of igf1 and slin? I have used IGF1 many times and dont get light headed/hypo if I eat or not when i take igf... who knows if it was just long acting slin... be careful buddy!
MT2 is one that is very stable. I would not worry to much with mt2, ofcourse still trust your place somewhat and look at reviews always : )

[XxAndreaxX]

Assuring what location they are made in does not tell you how its made... you could ask but unless you got some advanced facilities at your disposal... trust and track history sadly what you go by on top of what they say..


I have seen them at many place and judging from what I have read and some of price I would lean towards they are just a reseller of Chinese made peps and who knows on quality... but my main concern with your question is that Clen and AI's are not peptides... so this thread doesn't apply in the same way.

while this is tru some can be made both ways. as IGF1 could... it doesnt mean its functional as it would be made by recombinant dna. some would seem to have full action and others would be sub-par IMO.


Thanks!! : )

100% agree! why I avoid all these new places that pop up or been around for only a couple years... go with a long track recired and places that seem to know what the heck they are talking about... trust me I got in arguments with some pep sellers that would not answer my basic questions or gave me "bro logic" answers they clearly just read online and did not deeply research... its a mess in the pep / RC game for sure!


what do you mean sides of igf1 and slin? I have used IGF1 many times and dont get light headed/hypo if I eat or not when i take igf... who knows if it was just long acting slin... be careful buddy!
MT2 is one that is very stable. I would not worry to much with mt2, ofcourse still trust your place somewhat and look at reviews always : )

I tell you my sides on IGF-1 LR3... hypo all day, stronger after pin. Very very tired, slept very good all night and difficult to get up in the morning. Towards the end of the cycle I got vitreous deteachment and as result miodesopsies.
No fat loss, and maybe some signs of carpal tunnel, but I’m not shure.

[gold43]

I tell you my sides on IGF-1 LR3... hypo all day, stronger after pin. Very very tired, slept very good all night and difficult to get up in the morning. Towards the end of the cycle I got vitreous deteachment and as result miodesopsies.
No fat loss, and maybe some signs of carpal tunnel, but I’m not shure.

Those are not IGF-1 sides.