i had to put my test in glass vile then put in pan of water with pin in top to vent,then would swirl every few minutes,water was almost at a boil ,i probably took the oil to about 195 degrees,before i cooked the pain was so bad about 1 to2 days after shot weather in glut or quad it was hurting my work out routine,bad pain.any way after cook i did shot 2 days ago no pain yet at all, i love it,but i hope the heat did not kill the active ingrediant,any body have any input.