
Originally Posted by
Cylon357
You are filling up to the .15 mark or the 1.5? Because the first is easy, that second one... that would be tough!
Here is how I do the backfilling thing that works up to about .65ml, but does work WAY better with lower doses (less than .40 or so)
Don't try to judge how much is in the syringe you are backfilling INTO (now known as B), rather by how much you have pushed out of the syringe you are backfiling FROM (now designated as A).
Pull the plunger(s) out of syringe(s) B. Lay them on a clean surface, I usually make sure the shafts of the plunger is laying on the alcohol wipe I used to clean the top(s) of my goods, but not the stopper itself. You DID remember to wipe your tops, right?
Draw as much as you want of your material into syringe A. Make note of where you draw to.
In this example, I will use a total draw of .50ml with .10ml needed in each syringe.
Pickup the first of your syringe B, then slowly and carefully inject from A until the plunger is at the .40 mark. This means that you have moved .10ml into syringe B.
I usually try to make sure the substance doesn't close up the syringe B... This is hard to explain, but imagine looking at B while it is laying on its side. If there is room for air to get around the material, you have done this right.
Carefully pickup up B and tilt it at a very slight angle, plunger end down, needle in up. Slide the plunger into syringe B slowly. As you slide it in, tilt it needle end up. All the peptide or whatever should fall down to the plunger. Push out the remaining air, but leave about .01 or .02 ml air.
Tilt B needle side DOWN, and flick it lightly once or twice to get the air against the plunger.
Repeat for all B syringes.
Once complete, store needle side down in a glass (I use a tall shot glass) and place in the refrigerator if needed.
That's it. One piece of advice: use 1.0ml syringes for B. Lower volume syringes, like .50 or .30 tend to have more of the situation where you can't get all the air bubbles out of B.
It can take some time to get right, but once you do it is easy peasy. Good luck.