What is HGH made from??

[matt77]

As the title says, just curious as to what they make synthetic HGH from. I know its probably a lot of technical stuff so just the basic info would keep my brain from hurting today.

While on the subject of chem. what would be a good review? Im thinking of retaking college chem and physics one class at a time for a good review. It kinda makes me sick feeling to say that!

[mannimal]

Certain genetic sequences that code for HGH are inserted into E. coli in hopes that the bacteria will pick up the chain and begin reproduction. The results, if successful, are collected and processed.

[frawnz]

Recombinant DNA modification:

Plasmids are extrachromosomal self-replicating circular forms of DNA present in most bacteria, such as Escherichia coli (E. Coli), containing genes related to catabolism and metabolic activity,[1] and allowing the carrier bacterium to survive and reproduce in conditions present within other species and environments.

These genes represent characteristics of resistance to bacteriophages and antibiotics[1] and some heavy metals, but can also be fairly easily removed or separated from the plasmid by restriction endonucleases,[1], which regularly produce "sticky ends" and allow the attachment of a selected segment of DNA, which codes for more "reparative" substances, such as peptide hormone medications including insulin, growth hormone, and oxytocin.

In the introduction of useful genes into the plasmid, the bacteria are then used as a viral vector, which are encouraged to reproduce so as to recapitulate the altered DNA within other cells it infects, and increase the amount of cells with the recombinant DNA present within them.

[matt77]

Thanks guys.