IGF1 Reconstitution?

[Cycleon]

looking at what the best method is? i have lyophilized versinon in 1mg containers - I know it prefers an acidic environment but what is best to be able to shoot?

[Da Bull]

I've only used HCL CYCLEON...couldn't say otherwise,but it didn't give me a problem.

[Cycleon]

straight HCL? what? then portion out an amount into sterile water and shoot? I am not sure I like the idea of shooting HCL IM

[Da Bull]

Check your pm bro.

[einstein1905]

I'm going with the 10mM HCl this next time. Even cutting with water when it's in BA is still more sting than is necessary.

[Cycleon]

and for the lazy - 10mM of HCL for 5ml of water is how much HCL?

[einstein1905]

and for the lazy - 10mM of HCL for 5ml of water is how much HCL?

4.167 micro liters. I'm assuming you have access to "stock" HCL (12M).
If you have access to micropipetors to measure that small of a volume, no big deal. If you don't, I'd dilute the stock HCl 1/50 then you'd need 208.3 microliters, which you can get fairly close on a slin pin. the final pH will be much closer to ideal than with straight BA even if you don't get the exact right measurement with the slin pin.
This is assuming the total volume is 5mL. I mean 4.167uL HCl added to 4.99583mLs water

[Cycleon]

I can get a pipettor easy enough but I dont feel like it since I get my stuff tommorrow - I will probably go with the dilute and put .2cc in the slin pin - good call on it.

[einstein1905]

I can get a pipettor easy enough but I dont feel like it since I get my stuff tommorrow - I will probably go with the dilute and put .2cc in the slin pin - good call on it.

It would be nice to be in the position to be able to reconstitute some IGF-1, if you know what i mean

[Cycleon]

well, when you can get it from the getting place it does help - unfortunately, I dont get it from the down under place or I would be richer still. It do help to be in the right business tho

[Cycleon]

einstein - what if I am using .1N HCL solution? - thats all I have about -

[einstein1905]

einstein - what if I am using .1N HCL solution? - thats all I have about -

Easier yet for you then.
0.5mL of your 0.1N HCl and to 4.5mL of water will be 10mM

[dIeSeL_225]

heres some info on how to reconstitution, some agree others dont
http://anabolicreview.com/vbulletin/...&highlight=igf

[Cycleon]

and just for kicks - If I want to go with acetic acid?

[einstein1905]

and just for kicks - If I want to go with acetic acid?

Is this some kind of test?
I'm assuming you have concentrated acetic acid (glacial). If so, to get the same pH as 10mM HCl, you need 32.84% acetic acid. So, if you're going with the 5mL total volume, 1.64mL acetic acid with 3.36mL of water
That ought to do it.

[Cycleon]

you are my new bestest frieeeeend - can you say frieeeennndd? There! I knew you could - no I just have the HCL elsewhere and cant get over to it till monday - but I want to start tonight so Im running to the store to get my vinegar and run it through my filter

and I am having to deal with too much board junk today to try to do the math myself