Atomini's NOOPEPT log!!!

[Atomini]

Guys, I have stumbled across some very interesting findings in regards to route of administration (sublingual vs oral). I will post it later as I am out and about right now with school and all. For now, I will say this: Edwin, try dosing your Noopept sublingually and report back and let me know how it goes.

[lovbyts]

I was reading the same last night and it seems this is the prefered method. Ill start with my dose this morning.

[krugerr]

How comes the sub-lingual administration gives better results? Is it because it is absorbed slowly, rather than digested?

[Atomini]

How comes the sub-lingual administration gives better results? Is it because it is absorbed slowly, rather than digested?

It is actually absorbed faster sublingually and goes directly into the bloodstream in its unaltered form. The problem is that even though the peptide is small enough to be abosrbed through the GI tract, it seems as though some portion of the Noopept still undergoes some kind of modification, so here we go...

IMPORTANT INFORMATION ON THE ADMINISTRATION OF NOOPEPT - MUST READ!!

Alright, so i've experimented with dosing Noopept in 3 different ways:

- Oral administration
- Sublingual administration
- Neublizing/vaporizing it

After plenty of research and trying primarily oral and sublingual administration, i've found that sublingual administration by far produces a greater immediate effect and an overall better effect than oral administration (even though it has been found in clinical testing that oral administration provides 95% or higher absorbtion. I've also tried nebulizing/vaporizing it but haven't done this enough to be able to gauge any effects properly. The problem is like I said, Noopept seems to undergo a few changes due to enzyme activity and also a few things in regards to the liver metabolism. A simple google search will yield quite a bit of information in the form of personal experience as well as commentary by some people who really know their chemistry well.

Noopept is also highly soluble in alcohol, and while sublingual administration of the powder or tablets directly under the tongue works well, many reccomend mixing it into a very small amount of alcohol (yes, hard liquor or something - you don't need much at all) and then let it rest sublingually under the tongue for maximum transport efficiency into the sublingual membranes. I have not yet tried this but I will some time soon and report my findings. One such study has demonstrated that Noopept is absorbed into the blood stream through the GI tract and crosses the blood brain barrier unchanged(1) while another study demonstrated that Noopept ALSO undergoes hepatic metabolism (liver metabolism) into a metabolized form. So, I think we can conclude pretty distinctly that a good portion of Noopept is absorbed right into the bloodstream through the GI tract but a good portion of it is also metabolized in the liver!

Here is what's actually going on from what i've gathered and from my knowledge of biochemistry in my analysis of the Noopept molecule (I actually hadn't examined it very closely until now): Noopept is a modified polypeptide consisting of an ester (Phenylacetyl ester) and 2 amino acids: Glycine and Proline. It is basically the amino acids Glycine and Proline linked together, with an Phenylacetyl group esterified to the Proline. This forms a peptide molecule, but an extremely small one and with the additional modifications of an ester attached to the amino acids, it is a unique one.

I will explain this better, as usual, with my photoshopped pictures and explanations for those who may be considered a layman when it comes to chemistry and biology:

Here is the amino acid Glycine:



Here is the amino acid Proline:



Here is the complete Noopept molecule:



Here is a breakdown and explanation of what is where and what is what within the Noopept molecule:




So, what does this mean and how does it all add up? Well, first of all, peptides when administered orally are rendered, for the most part, useless. This is because digestive enzymes and acids will rip apart the polypeptide (AKA a protein) into its individual amino acids for use. This is why we cannot ingest HGH, IGF-1 or any of these long-chain amino acids. They are too large to enter the gated channels in the cells lining the GI tract to be absorbed, so they must be broken apart into their smaller amino acids and be taken into the body that way. What results is a peptide that is no longer functional because it got ripped apart and digested. Now, smaller peptides such as Noopept and single-amino acid molecules such as T3 (Triiodothyronine) are absorbed at a much higher rate because of how small the peptide chain is (T3 is simply one amino acid with iodine atoms attached to it, and Noopept is just 2 peptides large with an ester attached to it). Alright, we already know these details, so what? Well here is what: a certain percentage of peptides usually make it through the GI tract into the bloodstream intact (especially small ones like Noopept). However, every individual is different in how they process different foods, medications, and compounds, and so and there are a large variety of enzymes that break the bonds between amino acids in peptide chains into the individual amino acids. When it comes to peptides, it then presents an issue as to how to generate an effective solution for dosing. This is whyother methods of administration are used. When it comes to the REALLY big peptides like HGH, IGF-1, etc. they must be injected (or nasal sprays, etc.) and there also exist transdermal administration for other peptide-based bedications. For small ones like Noopept, individuals can use sublingual administration for increased absorbtion. But how does this work? And how does Noopept still retain oral bioavailability in the GI tract? Well, it's time to delve deeper into that!

One must understand that if a specific percentage of Noopept (or any compound) demonstrates bioavailability through the GI tract and crosses the blood brain barrier unchanged, it doesn't mean that it underwent 100% absorbtion. Compounds like Noopept do not require 100% absorption to operate efficiently due to the fact that this is SUCH A STRONG RACETAM (1,000 times STRONGER than the strongest Racetam that was around before it). Now here is the other thing, in addition to having a certain percentage of it absorbed through the GI tract, any additional Noopept that was not absorbed in this manner will continue on to the hepatic system (the liver) and undergo hepatic metabolism where it may be broken down in the liver into metabolites, BUT this would not affect the Noopept that did cross the blood brain barrier, because it remains unchanged by hepatic metabolism (the amount that gets through to the blood brain barrier intact does not reach the liver at all as it avoids the first pass).

It is very important to understand that Noopept's mechanism of action is that of a pro-drug. NOOPEPT DOES NOT DO ANYTHING DIRECTLY. Its metabolites are the active drugs that do the work. What this means is that the administration of Noopept seems to increase the concentration of an endogenous Nootropic in the nervous system known as Cyclo-L-prolylglycine(3). If you still don't understand what this means, it is basically the same idea as prohormones or HGH. When exogenous HGH is administered, its half life is extremely short but the mechanism of action of HGH is that it signals the liver to release large amounts of IGF-1, which is the hormone that actually does the work that is commonly credited to HGH. Similar concept with Noopept. Noopept could be considered a propsychotropic just like how 1-androstenedione is a prohormone. REMEMBER THIS! IT IS VERY IMPORTANT!

The Noopept molecule as a whole, from what I have been able to gather from my research, works like this: The section of the Noopept molecule that seems to actively provide the effects of Cyclo-L-prolylglycine increases is most likely the Racetam part of the Noopept molecule (the 2-Pyrrolidone ring), and if you don't know what that is then just scroll up and look at the Noopept molecule. The 'Racetam' part of the molecule is the four corner ring with the Nitrogen (N) atom right at the top center of it (this ring is officially known as a 2-Pyrrolidone ring in chemistry). This ring is the one common structural characteristic among all Racetams that make Racetams a Racetam. Just like how all anabolic steroids possess the basic four-ring carbon structure known as the steroid structure - this is what makes a steroid a steroid. Now, Noopept may also work on Glycine receptors using the tail end of the Noopept molecule as well (the tail end if you take a look at the picture IS the Glycine amino acid). The whole Noopept molecules does need to be intact for it to do its job. Individually ripped apart Glycines, Prolines, and Phenylacetyl esters floating around won't do anything in the body - its like ripping a car apart into individual nuts, bolts, and scaps of metal. It's not even a useable car at that point!

So, the question is: Oral or sublingual administration?

ORAL: Conclusion is that a good portion IS absorbed orally, while a good portion is also destroyed by enzymes and hepatic metabolism. The reason why Noopept still works so well even when ingested orally is because it's such a powerful compound (remember, 1,000 times more potent than Piracetam, which was the strongest Racetam that was around before the discovery of Noopept).

SUBLINGUAL: You will absorb almost 100% through this route. Not much else to be said here. No risk of any percentage of it getting ripped apart by hepatic metabolism, as it avoids the first pass. KEEP IN MIND THAT DOSING NOOPEPT IN THIS MANNER WILL RESULT IN FAR STRONGER AND MORE POTENT EFFECTS IN COMPARISON TO ORAL ADMINISTRATION OF THE SAME DOSE (30mg orally vs 30mg sublingually will = far stronger effects sublingually), AND THEREFORE A LOWER DOSE MAY BE NECESSARY DOING THIS.

However, at the end of the day, the fact of the matter is this: people who take decide to take Noopept or racetams that are waiting to expect some massively huge effect need to drop the Noopept and look somewhere else. Racetams and Noopept cause changes that many people just take for granted or will not truly notice. You have to not only be in proper tune with your body and its reactions, but you also need to engage your brain in constant learning stimulus to really notice the difference between after taking Noopept and before you started. These effects from Noopept, as I have said MANY TIMES, are cumulative (meaning, they build-up slowly over time just like AAS and you cannot expect 'gains' within the first week or two of use)! I cannot hammer that point enough into everyone's heads here. DO NOT expect anything short-term wise from Noopept unless you are a very sensitive responder. My effects did not reach a higher level of effects until at least 2 weeks into use.

REFERENCES:
1. Pharmacokinetics of new nootropic acylprolyldipeptide and its penetration across the blood-brain barrier after oral administration. Boiko SS, Ostrovskaya RU, Zherdev VP, Korotkov SA, Gudasheva TA, Voronina TA, Seredenin SB. Laboratory of Pharmacokinetics, Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow. Bull Exp Biol Med. 2000 Apr;129(4):359-61.
2. Bioaccessibility of the new dipeptide nootropic drug noopept. S. S. Boiko, V. P. Zherdev, T. A. Gudasheva, R. U. Ostrovskaya, S. A. Korotkov, O. Yu. Kravtsova. Khimiko-Farmatsevticheskii Zhurnal, Vol. 38, No. 12, pp. 3 – 5, December, 2004.
3. "The major metabolite of dipeptide piracetam analogue GVS-111 in rat brain and its similarity to endogenous neuropeptide cyclo-L-prolylglycine". Gudasheva TA, Boyko SS, Ostrovskaya RU, Voronina TA, Akparov VK, Trofimov SS, Rozantsev GG, Skoldinov AP, Zherdev VP, Seredenin SB (1997). European Journal of Drug Metabolism and Pharmacokinetics 22 (3): 245–252. PMID 9358206

[lovbyts]

How comes the sub-lingual administration gives better results? Is it because it is absorbed slowly, rather than digested?

Bypasses the liver so more is absorbed.

[krugerr]

Bypasses the liver so more is absorbed.

Thank you!

[Edwin23q]

Guys I took supposedly 100mg last night before bed and had the most Fuked up dream I've ever had in my life, I have no idea wtf was going on but I jumped up at 3:00am screaming "why did you let that dog in" my wife jumps out of bed and says THAT'S IT. YOUR BANNED FROM GETTING ON ANOTHER CYCLE", rofl I then started laughing like a retard and went back to sleep and it wouldn't have been that long before the dream continued from that exact spot until my alarm rang.
I have no idea what caused this dream as I hardly ever dream and never like that.
I think the noopept I'm taking is severely underdozed and only notice it when I take a large dose, or maybe it was a coincidence.
I'll take another 100mg at work and lets see what the effects are while awake.

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[boz]

Guys I took supposedly 100mg last night before bed and had the most Fuked up dream I've ever had in my life, I have no idea wtf was going on but I jumped up at 3:00am screaming "why did you let that dog in" my wife jumps out of bed and says THAT'S IT. YOUR BANNED FROM GETTING ON ANOTHER CYCLE", rofl I then started laughing like a retard and went back to sleep and it wouldn't have been that long before the dream continued from that exact spot until my alarm rang.
I have no idea what caused this dream as I hardly ever dream and never like that.
I think the noopept I'm taking is severely underdozed and only notice it when I take a large dose, or maybe it was a coincidence.
I'll take another 100mg at work and lets see what the effects are while awake.

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LOL oh man literally laughing here. You havent done yourself any favours there ed. GIve it maybe a couple more tries mate, i reckon throw it out, sounds like there dealing garbage the suppliers. I know atomini has stated give it time, in all honesty though i have not seen one positive post from you since you have been on it. If my memory servers me correctly ill re check, atomini was feeling positive effects within days.

[Edwin23q]

LOL oh man literally laughing here. You havent done yourself any favours there ed. GIve it maybe a couple more tries mate, i reckon throw it out, sounds like there dealing garbage the suppliers. I know atomini has stated give it time, in all honesty though i have not seen one positive post from you since you have been on it. If my memory servers me correctly ill re check, atomini was feeling positive effects within days.

Lol, true that. Just ordered from the suppliers I was PM'd with and also a precision scale to measure dosage, I'll stop taking these so called caps and wait for the delivery.

By the way I just opened a cap to taste the contents and it tasted kinda sweet but bitter at the same time, but that was it. It was a chalkie texture that when put under my tongue turned into putty like and stayed there and wouldn't dissolve, I had to scrape it out which was annoying as it was all stuck to my teeth.

Your thoughts on this?

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[hammertimebaby]

the caps form bulknutrients contains poly dextrose, which is just a filler and undigested source of carbs. I use the caps and they are no good at all. i guess u get what you pay for. I will order some powder from bulknutrients and some from ebay and compare.

[Atomini]

I have a feeling this would get better responses if that post was its own new thread. I have a feeling that with that post being on page 8 of this thread, its going to quickly get lost and people will miss it when checking out this thread for info on Noopept...

[Edwin23q]

I have a feeling this would get better responses if that post was its own new thread. I have a feeling that with that post being on page 8 of this thread, its going to quickly get lost and people will miss it when checking out this thread for info on Noopept...

I think you should write up a sticky based on your previous posts.
Your right as too many replies will cause people to skip pages and just read the last responses. It will get annoying as you'll get the same questions you've addressed over and over again.

Sorry mate, I've probably stuffed it up more than anyone else, lol

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[lovbyts]

Well last night/this morning my sleep quality wasnt so good as it has been. partially due to my schedule but when I got to sleep I woke up within 2 hrs and I could tell I was not going to go back to sleep easily. I lay there for a while wondering what was wrong and thinking about taking 1/2 an ambien. Then it hit me. I forgot to take the Noopept when I got home....

I got up and went out and took my noopept. I did it sublingual this time. Yeah I agree with Atomini, taste like a$$ or what I imagine it would taste like. I went back to bed and it took a little while but after I went back to sleep I stayed asleep for the next 5hrs.

[lovbyts]

the caps are going to be mostly filler because 30mg of noopept is very little. The powder comes with a LITTLE spoon that is about the size for a mouse or something the size of a ferry would use. lol Really its small and you are supposed to use 1-3 spoon fulls but I think it's more like 6. My scale is not sensitive enough so I need to order a new one also.

Here is a pretty good example.

[krugerr]

Here is a pretty good example.
<img src="http://forums.steroid.com/attachment.php?attachmentid=132719"/>

How big is that coin? What coin is it?

[Atomini]

the caps are going to be mostly filler because 30mg of noopept is very little. The powder comes with a LITTLE spoon that is about the size for a mouse or something the size of a ferry would use. lol Really its small and you are supposed to use 1-3 spoon fulls but I think it's more like 6. My scale is not sensitive enough so I need to order a new one also.

Here is a pretty good example.

LOL CANADIAN PENNIES!!!

And yeah, I saw the stupid tiny spoon that came with my powder, I looked at it and I was like "WTF is this shit!?" and threw it away. They claim each scoop is 10 grams. BULLSHIT! I trust my scale, not some stupid spoon meant for ants and spiders. In fact, the tiny spoon/scoop is SO small that when you scoop out a spoonful, you can't even shake the powder out of the spoon, it gets stuck in such a small spoon.

The size of the powder in relation to the pennies is a perfect example of how much Noopept powder measures out to the different amounts. Those look spot-on, same amounts it comes to on my scale pretty much.

[boz]

Ive been looking into how im going to weigh up my powder, thanks for that pic lov i think i really am going to have to invest in scales though. Im just a little confused of the conversion from g to mg. Never heard of scales weighing up by the mg, only grams, and than you have to do the maths yourself right?

[krugerr]

Ive been looking into how im going to weigh up my powder, thanks for that pic lov i think i really am going to have to invest in scales though. Im just a little confused of the conversion from g to mg. Never heard of scales weighing up by the mg, only grams, and than you have to do the maths yourself right?

1000mg = 1g

So 30mg = 0.03g

[boz]

1000mg = 1g

So 30mg = 0.03g

Good man cheers.

[Edwin23q]

Picked this up on eBay, it seems pretty good;









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[lovbyts]

Picked this up on eBay, it seems pretty good;


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That will work and there are several on amazon as well.
http://www.amazon.com/American-Weigh...ital+scale+1mg

How big is that coin? What coin is it?

It's a Canadian penny $0.01 same size as a US penny.

[BlueWaffle21]

That will work and there are several on amazon as well.
http://www.amazon.com/American-Weigh...ital+scale+1mg




It's a Canadian penny $0.01 same size as a US penny.

I took the plunge and ordered some last week and I also got the scale posted above, it works GREAT! This is my second day taking it and I can't believe how much energy I have!!! I wake up at 4:00 a.m. and hit the gym at 5, when I get home I usually nap for a couple hours then get grinding on school..........today there was no nap needed!!!!! Hopefully this is a great sign of the things to come. Thank you Aomini, always on point!

[ElDude]

That will work and there are several on amazon as well.
http://www.amazon.com/American-Weigh...ital+scale+1mg




It's a Canadian penny $0.01 same size as a US penny.

Got the same scale myself last week, works great! Still waiting for this damn Noopept to get in though!!! Yeesh, I ordered 10g of it 11 days ago and still nothing. The place I ordered it from has the shittiest customer service ever and won't get back to me!

[RaginCajun]

check this out yall! i remember watching this back in the college days and it reminded of this thread!

you will be able to tell who Atomini is!

http://video.adultswim.com/sealab-20...stimutacs.html

[Edwin23q]

Lol the influx on orders of precision scales has the governments worried that Cocain dealers are on the increase, lol

I threw my play dough (fake noopept caps) in the bin, more money wasted on bunk supplements.
I ordered my next batch a few days ago from a recommended supplier and now I play the waiting game.

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[jasc]

That will work and there are several on amazon as well.
http://www.amazon.com/American-Weigh...ital+scale+1mg

Thanks for making that easy LB. Been rackin my brain with a few diff mg scales n this one sold me.

[Zodiac82]

Got the same scale myself last week, works great! Still waiting for this damn Noopept to get in though!!! Yeesh, I ordered 10g of it 11 days ago and still nothing. The place I ordered it from has the shittiest customer service ever and won't get back to me!

lol same here I ordered 9 days ago and I just got an email today thats its been sent out today

[Brick]

lol same here I ordered 9 days ago and I just got an email today thats its been sent out today

I've got good customer service but still takes forever! Tracking says it just got processed in Vancouver, CAN so I should have tomorrow or Wednesday!

[ElDude]

lol same here I ordered 9 days ago and I just got an email today thats its been sent out today

Where did you order from? I am not sure if we can ask/list sources on here? I am assuming so as noopept is legal. I ordered mine from Elevated Labs. Still have not gotten anything from them and it has been 13 days. Ordered noopept on Jan 18th, got an e-mail from them saying it shipped on the 23rd. On the 29th i got an e-mail from them saying it never shipped and that they were re-shipping it that day. Never got a confirmation e-mail that it shipped and still haven't heard from them. Do not order from Elevated Labs, they suck. Posted a comment on their FB page about their shitty service, they took the post down and banned me from their page.

[evander87]

Where did you order from? I am not sure if we can ask/list sources on here? I am assuming so as noopept is legal. I ordered mine from Elevated Labs. Still have not gotten anything from them and it has been 13 days. Ordered noopept on Jan 18th, got an e-mail from them saying it shipped on the 23rd. On the 29th i got an e-mail from them saying it never shipped and that they were re-shipping it that day. Never got a confirmation e-mail that it shipped and still haven't heard from them. Do not order from Elevated Labs, they suck. Posted a comment on their FB page about their shitty service, they took the post down and banned me from their page.

HUGE Thank you for a heads up. I ordered a bottle, to test out, from Amazon and was planning on ordering bulk from EL. Thanks you for the heads up. I'll look elsewhere.

As far as effects for me. I have a problem with my mind going faster than my mouth in conversations. Often stumbling at some point and it's as if the "wheel just fell off my train of thought" and I become a loss for words. However a day day or so after starting I've been able to find and use my words more effectively in conversations. That's awesome. I still don't find myself sleeping better or better focus. I started a couple online classes and I find myself drifting off to other thoughts.

A footnote, I often awake in the middle of the night and my mind starts thinking about what I need to do the next day. It feels like a panic attack, heart racing, elevated adrenaline, etc, but since starting NOOPEPT I still awake with thoughts of what I need to do the next day but I'm much calmer about it.

I'm very happy with what I'm finding with this stuff right now and will be buying the powder in bulk. Thanks Atomini for starting the brain revolution.

[OdinsOtherSon]

HUGE Thank you for a heads up. I ordered a bottle, to test out, from Amazon and was planning on ordering bulk from EL. Thanks you for the heads up. I'll look elsewhere.

As far as effects for me. I have a problem with my mind going faster than my mouth in conversations. Often stumbling at some point and it's as if the "wheel just fell off my train of thought" and I become a loss for words. However a day day or so after starting I've been able to find and use my words more effectively in conversations. That's awesome. I still don't find myself sleeping better or better focus. I started a couple online classes and I find myself drifting off to other thoughts.

A footnote, I often awake in the middle of the night and my mind starts thinking about what I need to do the next day. It feels like a panic attack, heart racing, elevated adrenaline, etc, but since starting NOOPEPT I still awake with thoughts of what I need to do the next day but I'm much calmer about it.

I'm very happy with what I'm finding with this stuff right now and will be buying the powder in bulk. Thanks Atomini for starting the brain revolution.

evander, did you get the powder from Amazon or the pre-made pill form? Thanks

[evander87]

evander, did you get the powder from Amazon or the pre-made pill form? Thanks

Oral dissolve pill. Thinking I might buy the powder from there as well though.

[boz]

I have been seeing alot of different capsuels sizes ranging from 0 - 5.

Which would be the ideal size if im weighing up 10mg caps? Or it doesnt matter?

[Atomini]

I don't even bother capping it any more, too much hassle. Besides, won't matter any ways now that I am sublingually dosing it all the time now.

[boz]

I don't even bother capping it any more, too much hassle. Besides, won't matter any ways now that I am sublingually dosing it all the time now.

In saying that, do you know which size would be ideal?

[Atomini]

I was never aware of what size caps I used. I honestly would buy a bottle of Jamieson brand time released Vitamin C (the one with the little beads in the capsule instead of the tablet), I would open up the capsule and just wash the beads down with water and then stick my Noopept into the capsule. It's a great way of re-using capsules but I don't know what size they are, but I was able to fit 30mg of Noopept into them.

[Atomini]

LOG UPDATE:

I just attempted to mix my Noopept with alcohol and tried to allow it to absorb sublingually. I have a left-over bottle of 108.2 proof (54% alcohol) bottle of Wild Turkey Rare Breed whisky. I measured my Noopept, dumped it into a shot glass, and added maybe a QUARTER of the glass with whiskey, stirred it up until the majority of the powder was dissolved, and then put it under my tongue.

Conclusions - I feel as though it is much easier to just sublingually dose the Noopept in its powder form rather than dissolve it in alcohol and do it, because the alcohol is much more difficult to remain under the tongue. The good news is that even if some or most of it did not absorb sublingually, whatever was swallowed should get through the GI tract quickly without being broken apart by enzymes or undergo hepatic metabolism. This is because Noopept is soluble in alcohol, meaning that the Noopept molecule contains unique properties that allow it to hold intermolecular forces and unique polarity (chemistry terms) that makes it highly attracted to the alcohol molecule. This means the alcohol should essentially 'force', 'push' or 'facilitate' the Noopept directly through the GI tract quickly into the blood fast enough so that the enzymes and acids don't destroy a lot of it, let alone it reaching hepatic metabolism.

Therefore, my conclusion is that mixing it in alcohol is good for better absorbtion through the GI tract via typical oral dosing. It is not good for sublingual dosing.

At the end of the day, I am going to just let the powder rest under the tongue. If I ever need to orally dose Noopept (through the GI tract), then I will mix with alcohol and drink it. I hope this update provided informative for everyone.

[Brick]

Just got my noopept.

Did a 30mg dosage

I apologize if you mentioned this before but I don't have time to sift through the pages (class soon). But what does it taste like? I put it under tounge and it tasted like shit. A bitter taste I would say. Is this correct?

[Brick]

I'm not sure if this is a placebo but it feels like my field of vision is wider. I mean this by even though I am looking at something (my phone for example) I notice and can pay attention to my surroundings better

[JimmySidewalk]

Yeah its bitter, mine tastes the same, funky smell to it too.
mine's from Phoenix Raw nutrition

So is that little spoon accurate or not ? i took 2 of those little micro spoons twice today. targeting 60mg. says in description it has between 10-15mg a level scoop