*Creatine blocks Myostatin*

[dec11]

Creatine is an intresting compound in respect to how it works.

Recent research has shed more light on this wonderful compound. It raises DHT and reduces Myostatin.

Myostatin up-regulation happens when one uses AAS at around 50-60days. So it may be wise adding Creatine at this stage to aid in additional gains.

wouldnt adding it in from the get go be way to go, rather than guestimating when myostatin up-regulation occurs?
great info btw swifto

[Swifto]

wouldnt adding it in from the get go be way to go, rather than guestimating when myostatin up-regulation occurs?
great info btw swifto

From clinical data, Myostatin up-regulation occurs between 50-60 days.







Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration

Kishore M. Lakshmana, Shalender Bhasina, , , Christopher Corcoranb, Lisa A. Collins-Racieb, Lioudmila Tchistiakovab, S. Bradley Forlowc, Katie St. Ledgerc, Michael E. Burczynskic, Andrew J. Dornerb and Edward R. LaVallieb

aSection of Endocrinology, Diabetes, and Nutrition, Boston University School of Medicine, Boston Medical Center, 670 Albany Street, Boston, MA 02118, United States

bDepartment of Biological Technologies, Wyeth Research, 35 Cambridge Park Drive, Cambridge, MA 02140, United States

cDepartment of Clinical Translational Medicine, Wyeth Research, 500 Arcola Road, Collegeville, PA 19426, United States

Received 31 August 2008; revised 19 December 2008; accepted 20 December 2008. Available online 21 January 2009.

Abstract
Methodological problems, including binding of myostatin to plasma proteins and cross-reactivity of assay reagents with other proteins, have confounded myostatin measurements. Here we describe development of an accurate assay for measuring myostatin concentrations in humans. Monoclonal antibodies that bind to distinct regions of myostatin served as capture and detector antibodies in a sandwich ELISA that used acid treatment to dissociate myostatin from binding proteins. Serum from myostatin-deficient Belgian Blue cattle was used as matrix and recombinant human myostatin as standard. The quantitative range was 0.15–37.50 ng/mL. Intra- and inter-assay CVs in low, mid, and high range were 4.1%, 4.7%, and 7.2%, and 3.9%, 1.6%, and 5.2%, respectively. Myostatin protein was undetectable in sera of Belgian Blue cattle and myostatin knockout mice. Recovery in spiked sera approximated 100%. ActRIIB-Fc or anti-myostatin antibody MYO-029 had no effect on myostatin measurements when assayed at pH 2.5. Myostatin levels were higher in young than older men (mean ± S.E.M. 8.0 ± 0.3 ng/mL vs. 7.0 ± 0.4 ng/mL, P = 0.03). In men treated with graded doses of testosterone, myostatin levels were significantly higher on day 56 than baseline in both young and older men; changes in myostatin levels were significantly correlated with changes in total and free testosterone in young men. Myostatin levels were not significantly associated with lean body mass in either young or older men.

Conclusion
Myostatin ELISA has the characteristics of a valid assay: nearly 100% recovery, excellent precision, accuracy, and sufficient sensitivity to enable measurement of myostatin concentrations in men and women.

Adding Creatine 10-15 days proir to that may help I guess.

Seeing the full paper of the Creatine/GASP-1 study would help.