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Thread: New Member, first cycle advice and help in transferring to sterile vial

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  1. #1
    So i have figured out from these posts I will use one size need 20-21 gauge to draw and already have a smaller needle in the vial to vent gases. When using a filter I willl remove the needle attach filter attach a new needle and then filter it into the vial. On the next packet I will remove the filter and redraw using the same needle as the first packet and then again reatach the filter and use a new needle. How many mL can a .22 filter do and is it necessary to get .22 over .4 Also I have to add the BA myself correct? after the vial is already full if this is correct I believe I read that its 2% of volume so 10cc will be .2ml. How does this sound

  2. #2
    Join Date
    Dec 2004
    Posts
    1,118
    Quote Originally Posted by Bonaparte View Post
    I NEVER said to bake it. I said autoclave (pressure cook) which is much safer, since you can't exceed 252 F. Ovens are unreliable.
    Ideally, you should draw up all the packets (of the same type) into a 60ml syringe, then use a caulk gun to filter the oil into the vial with a .22um syringe filter. If you do this, you could just let it sit for a week so that the BA kills anything that makes it through without worrying about heat-sterilizing.
    Quote Originally Posted by Finalforce View Post
    So i have figured out from these posts I will use one size need 20-21 gauge to draw and already have a smaller needle in the vial to vent gases. When using a filter I willl remove the needle attach filter attach a new needle and then filter it into the vial. On the next packet I will remove the filter and redraw using the same needle as the first packet and then again reatach the filter and use a new needle. How many mL can a .22 filter do and is it necessary to get .22 over .4 Also I have to add the BA myself correct? after the vial is already full if this is correct I believe I read that its 2% of volume so 10cc will be .2ml. How does this sound
    do what bonaparte says in bold for each AS you have. You should draw all of the cyp in 1 60ml syringe and then all the deca in 1 60ml syringe; then you will place a pin into the sterile vial you want to transfer to, rig your filter to the 60ml of cyp and filter; repeat using new filter for deca...

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