Once you're in no air can get in, the bubble that appears when you aspirate is just negative air. You can pull as far as you want but all you need is about .5 to 1 ml mark, wait few seconds then go to next step.
Once you're in no air can get in, the bubble that appears when you aspirate is just negative air. You can pull as far as you want but all you need is about .5 to 1 ml mark, wait few seconds then go to next step.
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