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Thread: Bacteria in gear causing PIP?

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  1. #1
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    Quote Originally Posted by Atomini

    Absolutely wrong!!!

    Sorry for making that so abrupt, but pharmaceutical companies combine both filtering methods AND autoclaves. An autoclave is a machine used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C for around 15-20 minutes depending on the size of the load and the contents. It is pretty much the equivalent of boiling it. Autoclaves are widely used in microbiology, medicine, tattooing, body piercing, veterinary science, mycology, dentistry, chiropody and prosthetics fabrication. They vary in size and function depending on the media to be sterilized. A medical autoclave is a device that uses steam to sterilize equipment and other objects. This means that all bacteria, viruses, fungi, and spores are inactivated. However, prions, such as those associated with Creutzfeldt-Jakob disease, may not be destroyed by autoclaving at the typical 134 °C for three minutes or 121 °C for 15 minutes.

    The other problem with your post is that you said that by heating anabolic steroids, the heat would destroy the hormone. This is also wrong. Anabolic steroids are such small and compact hormones with unique polarity, electronegativity, fat solubility, and other chemical properties that they require EXTREME HEAT to destroy them. For example, Testosterone's melting point is 155 °C (311 °F).

    You can boil a vial of anabolic steroids no problem, just make sure the heat does not reach 155 °C (311 °F). The boiling point of water is 100 °C (212 °F). Increasing the heat a little above that is no problem and will not damage the hormones.

    Peptide hormones are a different story. They will deform, denature and become damaged/destroyed VERY easily (especially the larger the peptide chain and resulting complex protein structure is).
    So you are saying they don't filter it? Because I've toured the compounding pharmacy I get my HRT test cyp from. And I know you are wrong. Do they heat? Sure. So did I when home brewing to get it to hold. Final filtering is what creates sterility. Period. In order to rely on heat alone to sterilize this compound for injection you would damage the hormone.

    Why do you suppose on my pharmaceutical grade test cyp it is in an amber bottle that clearly states to store away from light and heat?

    Additionally an autoclave doesn't just use heat. It uses pressure and heat. It is this combination that help make it effective. Also, the solvents alone should theoretically kill any living bacteria in the solution.

    If this guys gear is truly infected with bacteria to suggest heating in the oven as a reliable fix is a horrible suggestion.
    Last edited by swm1972; 01-30-2013 at 04:22 PM.

  2. #2
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    Quote Originally Posted by swm1972 View Post
    So you are saying they don't filter it? Because I've toured the compounding pharmacy I get my HRT test cyp from. And I know you are wrong. Do they heat? Sure. So did I when home brewing to get it to hold. Final filtering is what creates sterility. Period. In order to rely on heat alone to sterilize this compound for injection you would damage the hormone.

    Why do you suppose on my pharmaceutical grade test cyp it is in an amber bottle that clearly states to store away from light and heat?
    I did say they filter it.

  3. #3
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    Quote Originally Posted by Atomini

    I did say they filter it.
    Read edited version. I was typing while you were responding. Heat and pressure. He going to get that in his oven? Telling him this is a solution to bacteria laden gear is horrible advice.

  4. #4
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    Quote Originally Posted by swm1972 View Post
    Read edited version. I was typing while you were responding. Heat and pressure. He going to get that in his oven? Telling him this is a solution to bacteria laden gear is horrible advice.
    Of course, I have always told people that in order to properly sterilize gear, a Whatman filter of the appropriate size pores must be utilized. My response was intended to clarify the whole idea that the hormones can be destroyed by the heat of boiling water. Boiling water is not hot enough to destroy steroid hormones.

  5. #5
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    Quote Originally Posted by Atomini

    Of course, I have always told people that in order to properly sterilize gear, a Whatman filter of the appropriate size pores must be utilized. My response was intended to clarify the whole idea that the hormones can be destroyed by the heat of boiling water. Boiling water is not hot enough to destroy steroid hormones.
    Destroy? Perhaps not. Degrade? I'm not taking that chance.

    http://thyroid.about.com/od/thyroidd...gsinsummer.htm

    Not gear related, but there is a reason medications are labeled as avoid heat. And if a warm summer day can affect it what is 200F going to do.

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