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  1. #1
    system admin is offline Owner
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    Can a steroid test show if it is Steroids or a prohormone?

    Can a steroid test show if it is Steroids or a prohormone? We know that a steroid test will show the test levels, but with further research on the blood can they determine if it is actually an ilegal steroid?

  2. #2
    daem's Avatar
    daem is offline Anabolic Member
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    there are metabolites left in the blood stream from certain drugs like deca or EQ i believe.

    thats why those drugs can be detected for months after ceasing administration.

  3. #3
    system admin is offline Owner
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    But can the steroid blood test tell if the person is on a steroid or on a pro-hormone?

  4. #4
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    i believe so, I do not think the steroid test just shows for increased test levels, bc if you remember THG it increased test levels but was not found on the test, I believe the test see if you have the certain types of steroids in your system so I would thing prohormones would not be something that would come up positive.

  5. #5
    system admin is offline Owner
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    very true... Anyone else have info on this?

  6. #6
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    Oct 2003
    Quote Originally Posted by LightWeightBaby
    i believe so, I do not think the steroid test just shows for increased test levels, bc if you remember THG it increased test levels but was not found on the test, I believe the test see if you have the certain types of steroids in your system so I would thing prohormones would not be something that would come up positive.
    Where did you read that?
    I thought THG was just a "masked" form of trenbolone . (so it's metabolites are not discovered by the test while having the potency of Trenbolone).
    If test levels are elevated it shows up on the test no matter what!


  7. #7
    Carl Carlson's Avatar
    Carl Carlson is offline Junior Member
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    I believe prohormones show up as nandralone (deca ).

  8. #8
    Carl Carlson's Avatar
    Carl Carlson is offline Junior Member
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    Haha.....I hope this avatar shows up.

  9. #9
    mass junkie's Avatar
    mass junkie is offline banned
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    on the net
    It wont matter soon anyways...they'll both be considered illegal

  10. #10
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    Jun 2004
    Where I can find food.
    My apologies if the article is too long for a post, but I did the best I could to get it here intact from "The Journal of American Medicine". If you create a full account therre you can view the article (2000;284:2618-2621) in PDF form with tables, charts and link. I recommend the site to anyone who likes a good source of medical research. Heres the website

    Heres the article:

    Trace Contamination of Over-the-Counter Androstenedione and Positive Urine Test Results for a Nandrolone Metabolite

    Don H. Catlin, MD; Benjamin Z. Leder, MD; Brian Ahrens; Borislav Starcevic, MS; Caroline K. Hatton, PhD; Gary A. Green, MD; Joel S. Finkelstein, MD
    JAMA. 2000;284:2618-2621.

    The dietary supplement industry generates annual sales estimated at US $12 billion,1 and in 1998 up to $800 million of this was spent on "sports supplements."2 An important consequence of the 1994 Dietary Supplement Health and Education Act3 is that several androgenic steroids , eg, androstenedione, dehydroepiandrosterone, 19-norandrostenedione, and 19-norandrostenediol, became widely available over-the-counter (OTC) in the United States and via the Internet. This act enables companies to market steroids as nutritional supplements if they do not claim to diagnose, prevent, or cure disease, in which case they escape US Food and Drug Administration regulation. Thus, content and purity of the ingredients are left to the manufacturer.

    Athletes take OTC steroids for their alleged muscle-building properties. Often the package labels do not warn that they may contain substances that are banned by sports organizations and may cause a positive urine test result.
    Recently, there has been an increase in the number of athletes who have tested positive for nandrolone , a banned steroid popular in the early years of athletic doping. Since nandrolone itself is rarely found in urine, these cases are actually reports of findings of urinary 19-norandrosterone, the major but inactive metabolite of nandrolone (19-nortestosterone). Administration of nandrolone fell out of style in the 1980s because athletes know that it is readily detectable in urine for weeks.5 Our laboratory conducts approximately 15,000 tests per year and reported 10, 23, and 35 cases where 19-norandrosterone was found in urine in 1997 to 1999, respectively.
    19-Norandrosterone is also found in urine after ingestion of the OTC steroid 19-norandrostenedione.6 In sport, the origin of urinary 19-norandrosterone is moot because many sports organizations prohibit and test for OTC steroids in addition to controlled steroids. 19-Norandrosterone traces may occur naturally in the urine of untreated males7-8; therefore, the International Olympic Committee set cutoffs in 1998 at 2 ng/mL for males and 5 ng/mL for females.

    We recently reported that oral androstenedione administration increases serum testosterone and estradiol levels in young men.9 Because of reports suggesting that dietary supplement use can cause urine test results positive for 19-norandrosterone, we analyzed these men's urine samples for 19-norandrosterone. We also assessed the effect of administration of 10 µg of 19-norandrostenedione on urinary 19-norandrosterone excretion and examined the purity and content of commercial androstenedione preparations.


    Androstenedione Administration
    The subjects (n = 37, aged 20-40 years) and protocol have been described previously.9 The men were randomly assigned to receive no androstenedione (n = 13) or 100 (n = 13) or 300 mg/d (n = 11) of androstenedione (Sports One, Klein Laboratories, Wallingford, Conn) for 7 days. Urine was collected for 8 hours on days 0 (baseline), 1, and 7. Urinary 19-norandrosterone concentrations were compared on days 1 and 7 and between the 100- and 300-mg groups by paired t tests. Data are expressed as the mean (SD), and P<.05 was considered statistically significant.

    19-Norandrostenedione Administration
    Four healthy men, aged 30 to 44 years, who denied use of anabolic steroids and steroid supplements, participated in the study after giving written informed consent. Each man collected urine before and after ingestion of 10 µg of 19-norandrostenedione (>99.0% pure). The UCLA Office for Protection of Research Subjects approved the study.

    OTC Androstenediones, Standards, and Content
    Over-the-counter capsules were purchased from stores. Androstenedione, testosterone, 19-norandrostenedione, and nandrolone were obtained from Sigma Chemical Company (St Louis, Mo). The capsules' androstenedione content was determined by high-performance liquid chromatography.
    Impurity Analysis by Liquid Chromatography-Mass Spectrometry-Mass Spectrometry (LC-MS-MS) Impurities in excess of 1% or 0.1% of the androstenedione were detected by LC-MS-MS. We analyzed 18 capsules from brands 1 and 2 for steroid contaminants at more than 0.1% of androstenedione and selected Sports One (brand 1) for the androstenedione clinical study.9 We also analyzed 26 capsules from brands 3 through 9 for androstenedione content and traces of steroids (>1% of the androstenedione). Reanalysis for Impurities at 0.001%
    We reanalyzed 8 capsules from 4 bottles of brand 1 by LC-MS-MS for 19-norandrostenedione or nandrolone impurities at more than 0.001% of the androstenedione.

    Analysis of Urine Samples for 19-Norandrosterone and 19-Noretiocholanolone
    Steroid extraction and derivatization, with minor modifications, have been previously described.10 The analysis used a high-resolution mass spectrometer. Full scans of 19-norandrosterone were obtained on 10 samples with 19-norandrosterone concentrations greater than 5 ng/mL.


    Initial Androstenedione Capsule Analysis
    We analyzed 18 capsules from brands 1 and 2 to select a brand for the androstenedione clinical study. This analysis detected impurities at greater than 0.1% of androstenedione. Both brands were more than 99.9% pure, and brand 1 was selected (Table 1).

    Survey of Commercial Androstenediones
    We also analyzed 26 capsules from brands 3 through 9 at a less stringent level (1%) of impurity. Table 1 shows that brand 8 contained no steroids and brand 9 contained 10 mg of testosterone. The mean androstenedione content of the brands labeled as containing 100 mg varied from 85 to 103 mg. The amounts of androstenedione in brands 1, 3, and 4 were within ±10% of that stated on the label, whereas brands 2 and 5 through 9 contained less than 90%.

    Androstenedione Administration Study
    19-Norandrosterone was found in the urine samples of all subjects (n = 24; range, 1.3-23.1 ng/mL) who received androstenedione, in none of the baseline urine samples (n = 37), and in none of the day 1 (n = 13) or day 7 urine samples (n = 13) from the 0-mg/d group (Table 2). There was no difference in 19-norandrosterone concentrations between day 1 and day 7 (P = .06 and P = .67 for the 100 mg/d and 300 mg/d groups, respectively); thus, the mean concentrations of day 1 and day 7 were averaged. The mean 19-norandrosterone concentrations in the 100- and 300-mg/d groups were 3.8 (2.5) ng/mL and 10.2 (6.9) ng/mL, respectively. These concentrations differed (P = .006), indicating dose dependency (Table 1).

    View this table:[in this window][in a new window]
    Table 2. Number of Subjects Categorized by Concentration and Mean Concentration of 19-Norandrosterone in Urine Samples Collected 0 to 8 Hours After Administration of 100 or 300 mg of Androstenedione*

    Reanalysis of Brand 1
    After finding significant amounts of 19-norandrosterone in the urine samples from men who received brand 1, 2 capsules from each of 4 bottles of brand 1 androstenedione were reanalyzed using a method 100 times more sensitive to detect 19-norsteroids if present at a level greater than 0.001% of the androstenedione. None of the capsules contained nandrolone or 19-norandrostenediol; however, 7 of 8 contained 0.004% to 0.018% 19-norandrostenedione (mean, 0.0076% or 7.6 µg per capsule).

    19-Norandrostenedione Administration Study
    The urine samples of all 4 subjects who ingested 10 µg of 19-norandrostenedione contained both 19-norandrosterone and 19-noretiocholanolone (Table 3), neither of which was found in the baseline urine samples. The 8-hour recovery of 19-norandrostenedione, measured as equivalents of 19-norandrosterone and 19-noretiocholanolone, was 48% (range, 32%-66%) of the total 10-µg dose.

    View this table:[in this window][in a new window]
    Table 3. Mean Concentrations of 19-Norandrosterone and 19-Noretiocholanolone After Oral Administration of 10 µg of 19-Norandrostenedione to 4 Subjects


    We found 19-norandrosterone, a metabolite of the prohibited steroid nandrolone, in all urine samples obtained after oral administration of androstenedione and in none of the urine samples collected before androstenedione administration. The mean concentrations were small. Nevertheless, if these samples were from athletes, 20 of 24 men would have tested positive for 19-norandrosterone (International Olympic Committee cutoff, 2 ng/mL for males). Thus, the administration of androstenedione contaminated with 19-norandrostenedione results in 19-norandrosterone in the urine, as does the administration of nandrolone. However, the ingestion of 19-norandrostenedione as a trace impurity in androstenedione is readily distinguished from that of nandrolone use because the latter results in very high urine concentrations of 19-norandrosterone (>100,000 ng/mL),6 whereas in this androstenedione study the highest mean concentration was only 23.1 ng/mL.

    Brand 1 was selected for the clinical study because it had the most consistent content of androstenedione (Table 1) and less than 0.1% impurities. After the unexpected finding of 19-norandrosterone in urine, we retested brand 1 for steroid traces if present at a level greater than 0.001%, which could explain this finding. Only brand 1 was retested; thus, we have no data on trace contamination of other androstenedione preparations.
    The 19-norandrosterone detected in the androstenedione subjects' urine samples could result from metabolism of the administered androstenedione to 19-norandrosterone, stimulation of a latent metabolic pathway to produce endogenous 19-norandrosterone, or oral administration of the 19-norandrostenedione impurity in androstenedione. We cannot exclude metabolism of androstenedione to 19-norandrosterone. Aromatizable androgens such as androstenedione are readily converted to estrogens in humans, and this conversion is associated with C-19 demethylation to 19-norsteroids.

    We cannot exclude the possibility of stimulation of a latent pathway either. There are reports of up to 0.6 ng/mL of 19-norandrosterone in the urine of untreated males,7-8 of 19-nortestosterone and 19-norandrostenedione in human ovarian follicles,12 and of 19-nortestosterone in the serum of pregnant women.13 However, none of the 37 subjects who received androstenedione or the 4 subjects who received 19-norandrostenedione had 19-norandrosterone levels greater than 0.5 ng/mL in their baseline urine samples. These 41 cases together with the existing 47 cases7-8,14 add support for the suggestion that the upper limit of endogenous 19-norandrosterone production does not result in urine concentrations of 19-norandrosterone greater than 0.6 ng/mL.

    The hypothesis that the 19-norandrosterone is derived from the 19-norandrostenedione contamination is strongly supported by the finding that administration of 19-norandrostenedione produced urinary concentrations of 48.9 ng/mL of 19-norandrosterone and 16.7 ng/mL of 19-noretiocholanolone in the first 2 hours and a 48% recovery of the 19-norandrostenedione in urine as the metabolites 19-norandrosterone and 19-noretiocholanolone. Therefore, the 19-norandrosterone found in this study is almost certainly due to ingestion of contaminated androstenedione.

    The manufacturer of the particular batch of brand 1 androstenedione that we reanalyzed should not be faulted for a product with a maximum contamination of 0.018%. This level of contamination is far below the level of 0.1% accepted by the Food and Drug Administration for pharmaceuticals.15 In contrast, some of the other products contained less drug than was listed on the label and even misrepresented ingredients. According to the Dietary Supplement Health and Education Act,3 both brand 8 (no androstenedione) and brand 9 (fails to identify testosterone) are misbranded. If we apply the United States Pharmacopoeia16 criterion of ±10% for ethical pharmaceuticals, then brands 2, 5, 6, and 7 are misbranded (wrong strength). Thus, 6 of 9 brands tested were misbranded.

    Today most steroids are made from plant precursors, eg, diosgenin from yams and stigmasterol from soya, by semisynthesis17 or bacterial action.18 These publications do not provide sufficient detail to determine how the contamination of androstenedione with 19-norandrostenedione might have occurred. It is also possible that the contamination occurred after synthesis, for example, during capsulation or some other packaging or formulation step.
    Sport organizations that test for anabolic steroids have banned androstenedione even though it is sold OTC in the United States. The data reported herein show that contaminated androstenedione administration will, like nandrolone and 19-norandrostenedione, be detected as 19-norandrosterone in urine and can produce a positive test result for anabolic steroids in subjects taking OTC dietary supplements.


    Funding/Support: The work was supported by the National Football League, the National Collegiate Athletic Association, the US Olympic Committee, Major League Baseball, the Major League Baseball Players Association, and National Institutes of Health grants RR-1066 and DK02759. The funding organizations did not participate in the design, conduct, interpretation, or analysis of the study.
    Corresponding Author and Reprints: Don H. Catlin, MD, UCLA Olympic Analytical Laboratory, 2122 Granville Ave, Los Angeles, CA 90025 (e-mail:
    Author Affiliations: Departments of Molecular and Medical Pharmacology (UCLA Olympic Analytical Laboratory) (Drs Catlin and Hatton and Messrs Ahrens and Starcevic), Medicine (Dr Catlin), and Family Medicine (Dr Green), University of California, Los Angeles; and Endocrine Unit, Department of Medicine, Massachusetts General Hospital, Boston (Drs Leder and Finkelstein).


    1. Herbal treatments: the promises and pitfalls. Consumer Reports. 1999;64:44-48. MEDLINE
    2. Zorpette G. Sports supplements: bigger muscles without the acne. Scientific American Presents. 1999;10:52.
    3. Dietary Supplement Health and Education Act of 1994. Pub L No. 103-417, 1994. 103rd Congress, 2nd sess, S784.
    4. Angell M, Kassirer JP. Alternative medicine: the risks of untested and unregulated remedies. N Engl J Med. 1998;339:839-841. FULL TEXT
    5. Catlin DH, Murray TH. Performance-enhancing drugs, fair competition, and Olympic sport. JAMA. 1996;276:231-237. ABSTRACT
    6. Uralets VP, Gillette PA. Over-the-counter anabolic steroids 4-androsten-3,17-dione; 4-androsten-3beta,17beta-diol; and 19-nor-4-androsten-3,17-dione: excretion studies in men. J Anal Toxicol. 1999;23:357-366. ISI | MEDLINE
    7. Dehennin L, Bonnaire Y, Plou PH. Urinary excretion of 19-norandrosterone of endogenous origin in man: quantitative analysis by gas chromatography-mass spectrometry. J Chromatogr B Biomed Sci Appl. 1999;721:301-307. CrossRef | MEDLINE
    8. Le Bizec B, Monteau F, Gaudin I, Andrae F. Evidence for the presence of endogenous 19-norandrosterone in human urine. J Chromatogr B Biomed Sci Appl. 1999;723:157-172. CrossRef | MEDLINE
    9. Leder BZ, Longcope C, Catlin DH, Ahrens B, Schoenfeld DA, Finkelstein JS. Oral androstenedione administration and serum testosterone concentrations in young men. JAMA. 2000;283:779-782. ABSTRACT/FULL TEXT
    10. Catlin DH, Kammerer RC, Hatton CK, Sekera MH, Merdink JM. Analytical chemistry at the Games of the XXIIIrd Olympiad in Los Angeles, 1984. Clin Chem. 1987;33:319-327. ABSTRACT
    11. Fishman J. Biochemical mechanism of aromatization. Cancer Res. 1982;42(8 suppl):3277s-3280s.
    12. Dehennin L, Jondet M, Scholler R. Androgen and 19-norsteroid profiles in human preovulatory follicles from stimulated cycles: an isotope dilution-mass spectrometric study. J Steroid Biochem. 1987;26:399-405. CrossRef | ISI | MEDLINE
    13. Reznik Y, Herrou M, Dehennin L, Lemaire M, Leymarie P. Rising plasma levels of 19-nortestosterone throughout pregnancy: determination by radioimmunoassay and validation by gas chromatography-mass spectrometry. J Clin Endocrinol Metab. 1987;64:1086-1088. ABSTRACT
    14. Ciardi M, Ciccioli R, Barbarulo MV, Nicoletti R. Presence of norandrosterone in "normal" urine samples. In: Schänzer W, Geyer H, Gotzmann A, Mareck-Engelke U, eds. Recent Advances in Doping Analysis. Köln, Germany: Sport & Buch Strauss; 1999:97-104.
    15. US Department of Health and Human Services. Guidance for Industry ANDAs: Impurities in Drug Substances. Washington, DC: US Dept of Health and Human Services, Food and Drug Administration; 1999.
    16. United States Pharmacopoeia DI 2000, Micromedex, Vol III. Englewood, Colo: The United States Pharmacopoeial Convention Inc; 1998.
    17. Coppen JJW. Steroids: from plants to pills: the changing picture. Trop Sci. 1979;21:125-141.
    18. Merkli A, Christen P, Kapetanidis I. Production of diosgenin by hairy root culture of Trigonella foenum-graecum L. Plant Cell Rep. 1997;16:632-636.

  11. #11
    Anhydro78's Avatar
    Anhydro78 is offline Anabolic Member
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    May 2004
    I have wondered the same. Thank you for the good post!!!!!!

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