IGF LR3 "Nutral" reconstitued with AA

**myobolix** · 08-02-2005, 03:16 AM

This is what I heard about the brand:

"This brand "Nutral" comes in already reconstitued with Acetetic Acid and should be good for at least 2 years. Also it can be transported easy and without cooling, without the risk of being denaturated."

I personally do not believe these statements, and wanted some opinions.

**rodge** · 08-02-2005, 07:50 AM

i copyd this from another thread,its posted by johnyB,info given by gropep maufacterer of lr3.

Description
Long™R3IGF-I is a recombinant analog of human insulin -like
growth factor-I (IGF-I) that has been specifically engineered
for the enhancement of cell culture performance.
Long™R3IGF-I is more biologically potent in vitro than either
insulin or native IGF-I and has been documented to
significantly increase recombinant protein production. It is
ideal for both research and large-scale culture systems utilizing
serum-free or low-level serum applications. Long™R3IGF-I is
effective in any cell that contains type I IGF receptors, including
most Chinese Hamster Ovary (CHO) lines, hybridomas and
fibroblasts. Long™R3IGF-I is produced in a patented E. coli
expression system without the use of animal-derived
components.
Precautions
This product is for research/laboratory use. THIS PRODUCT
IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.
Storage

Long™R3IGF-I in lyophilized form should be stored at
2 to 8 C and is stable for at least 3 years. Liquid solutions of
Long™R3IGF-I (prepared as described below) can be stored
at 2 to 8 C and are stable for at least 2 years.
Preparation Instructions

1. Long™R3IGF-I is supplied lyophilized in an atmosphere of
nitrogen at a slight vacuum (-25 kPa). Remove the metal
cap from the glass vial and introduce an air filled syringe
through the septum to equalize the pressure.

2. Add sufficient 10 mM HCl or 100 mM acetic acid solution
to the vial to achieve a peptide concentration of at least
0.1 mg/mL. Concentrations of 1 mg/mL or more are
recommended.

3. Mix the solution thoroughly to insure the peptide is
completely dissolved.

4. Filtration of a Long™R3IGF-I solution or a culture medium
after the addition of Long™R3IGF-I should be performed
using a low protein-binding membrane such as
Polyvinyl Idene Flouride (PVDF), Poly Ethylene Sulfite (PES)
or Cellulose Acetate (CA).
Methods for Use

Once Long™R3IGF-I has been reconstituted, it can then be
added directly to cell culture medium. A titration of
Long™R3IGF-I should be performed for each different
application as the optimum concentration may vary depending
upon the cell type used and other components present in the
medium. The recommended final concentration range of
Long™R3IGF-I is 10 to 50 µg/L (or ng/mL).

Because Long™R3IGF-I and insulin may compete for the same
cell receptors, the effectiveness of Long™R3IGF-I will be
masked if it is added in conjunction with commonly employed
concentrations of insulin (1 to 10 mg/L). However, inclusion
of physiological levels of insulin (5 to 10 µg/L) in cell culture
medium containing the recommended levels of
Long™R3IGF-I results in beneficial synergistic effects in certain
applications.

-rodge

**rodge** · 08-02-2005, 07:51 AM

btw i never heard of the company nutral,anybody else?

-rodge

**michaelv** · 08-02-2005, 06:05 PM

Originally Posted by rodge nl.

btw i never heard of the company nutral,anybody else?

-rodge

I heard of it:
http://www.dutchbodybuilding.com/for...ad.php?t=42086

**myobolix** · 08-04-2005, 04:38 AM

Ok, so it can be stable for 2 years reconstitued...
But will it survive transport? Even if one does it at 2-6 °C?

**michaelv** · 08-04-2005, 05:42 AM

Originally Posted by myobolix

Ok, so it can be stable for 2 years reconstitued...
But will it survive transport? Even if one does it at 2-6 °C?

you can read the results at the link that I had posted

**myobolix** · 08-04-2005, 08:15 AM

Sorry but I do not find any answer in that thread. Just a lot of hype, and no common sense.
I firstly do not believe that reconstitued igf can be carelessly transported. Secoundly, why reconstitued, why not dry with an ampul of h2o extra? And why Acidic acid?
And above all, they say it is ok to store it at room t°!? Can you believe this?

I think someone is trying to sell big, no matter what.

IGtropin from Gensci is trasported in a dry lyofolised state, and an ampul of h2o extra.
The written info states that one should store it at refrigerator.
Common Sense.