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    Post Steroids DO make you agressive!! - Full Study

    32 Copyright © 2001 by MJM
    The Role of Testosterone in Aggression
    Katherine Simpson *† , B.Sc. (Hons.)
    ABSTRACT This review article explores the evidence that testosterone is significantly correlated with
    certain forms of aggression in a number of animals, although firm evidence is lacking for humans.
    Studies have revealed that structures within the limbic system are particularly involved in the
    elicitation of aggression and are sexually dimorphic. Testosterone can exert its effects in one of two
    ways: either on androgen receptors after conversion to 5-alpha-dihydrotestosterone or on estrogen
    receptors after aromatization to estradiol. It can act via genomic mechanisms to induce production of
    proteins or via non-genomic mechanisms to modulate neural activity. Androgen and estrogen receptors
    are also found along neurotransmitter pathways. As such, testosterone is able to modulate levels of
    various neurotransmitters that show evidence of mediating effects on aggressive behaviour. In addition,
    recent evidence suggests that these neurotransmitters are involved in processes such as olfaction and
    arousal and suggestions have been put forward explaining how testosterone may modulate these
    processes. However there is a critical time period early in life, usually within the first few days after
    birth, during which testosterone exposure is essential to elicit aggression in adulthood. It is thought that
    testosterone and its metabolites sensitize an androgen-responsive system, while estrogenic metabolites
    establish the capacity to fight in response to estrogenic stimulation later in life. Despite this, testosterone
    is only one of a myriad of factors that influence aggression and the effects of previous experience and
    environmental stimuli have at times been found to correlate more strongly.

    Changes in the concentration of hormones can have profound effects on mood and behaviour in humans.
    Variation in hormone levels have been implicated in some psychiatric disorders such as depression. For
    example, 50-75% of patients with major depression show hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis characterised by excess secretion of cortisol (1,2). Aggressive behaviour in society is a serious social problem. Between 1998-1999, the number of violent offences in England and Wales rose by 6.3%, the majority of which was violence against the person (83%) (3). Although aggression is affected by various influences, there is evidence to suggest that certain biological factors may modulate aggressive behaviour.

    This paper reviews the evidence supporting the role of testosterone as a biological factor affecting aggressive behaviour. Human studies have so far demonstrated a relationship between measures of testosterone in adolescent males and aggression (4) with similar results found in women (5). However, results from human studies have also been subjective (6-9) since results are based on psychological questionnaires and observation. For example, girls with a condition called congenital adrenal hyperplasia, in which androgens are secreted in large amounts, have been shown to act in a more rough-and-tumble way during childhood which was thought to correlate with aggression (10). In contrast, strong evidence for the role of testosterone in influencing aggression is available from animal studies.

    It is believed that testosterone mediates these effects during two separate periods in life. The first period is the ‘critical time period’, usually the first few days after birth during which sensitization of certain neural circuits is thought to occur. The second period occurs in adulthood perhaps through modulation of neurotransmitter pathways and is thought to depend on the earlier sensitization.

    This paper briefly reviews the evidence suggesting that testosterone modulates aggression in both males
    and females and is followed by a detailed discussion on the evidence which may shed light on the mechanisms involved. As such this paper focuses on effects during the critical time period shortly after birth and also later in adulthood, and also considers the brain regions thought to be involved in the androgen and estrogen responsive pathways. Relevant details regarding neurotransmitter pathways and other factors possibly involved are included to support the evidence.

    The term aggression has been defined as “a response that delivers noxious stimuli to another person” (11). In animals, however, there are various forms of aggression classified into predatory, intermale, fear-induced, irritable, territorial, maternal and instrumental (12). Androgens have been found to affect only certain forms, for example, intermale aggression which can be illustrated by the resident-intruder test. In contrast, predatory aggression is entirely independent of androgens (13). Therefore only the forms of aggression that have been found to have a link with testosterone facilitation will be discussed.

    The classical hormone removal and replacement experiment approach has shown a definite link between
    testosterone and aggression in animals. In general, castration leads to a decrease in aggression whilst
    replacement of testosterone restores the behaviour. However differences in the effects induced by
    testosterone have become apparent between males and females. Different regions in the brain modulate
    different hormone-dependent aggression (14) and females require longer exposure to androgens after
    ovariectomy to induce male-like behaviour (15).

    Male aggression
    In 1849 Berthold described behavioural changes in cockerels after castration and how these were reversed
    after the testes were replaced. Similarly it has been shown that aggression in castrated rats was restored to
    its previous levels by androgen replacement (16). More recently, intermale aggressive behaviour in rats induced by electrical stimulation of the hypothalamus was enhanced by androgens (13). Studies in rats, mice, monkeys and humans show that competitive or intermale aggression increases at puberty, whereas it is reduced by castration and increased by testosterone injection (13,16-22).

    Female aggression
    Aggression in females, like in males, appears to be facilitated by testosterone in a dose-dependent manner
    (16). In one study, ovariectomized female rats were given daily injections of testosterone, estradiol or a
    placebo. It was found that testosterone increased aggressiveness, measured by the frequency of fighting,
    whereas estradiol or a placebo had little effect (23). In general, female aggression patterns were similar to
    those seen in males, i.e. ovariectomy without hormone replacement decreased interfemale fighting and
    replacement of gonadal testosterone stimulated it (24). However, according to the authors, the presence of
    testosterone was not sufficient to ‘activate’ the aggression. Instead, the missing essential factor was
    likely to be an experiential event, for example, competition. Therefore it is possible that hormones are
    required for its persistence but are not sufficient to activate the neural circuitry required for aggression.

    Testosterone acts as a prohormone which when converted into 5-alpha-dihydrotestosterone (5a-DHT)
    acts on androgen receptors or when converted into estradiol by the enzyme aromatase, acts on estrogen
    receptors (Figure 1). There is overwhelming evidence that most of the effects of testosterone in mediating
    aggression occur after aromatization (33). For example, testosterone induced aggression is concurrent with an elevated level of aromatization and nuclear estrogen receptor activity in the hypothalamic/preoptic area.
    Treatment with an aromatase inhibitor blocked this aggression and lowered nuclear activated estrogen
    receptors (34,35). Furthermore the intensity of aggressive behaviour was directly correlated with the
    aromatase activity (p < 0.02) in the posterior hypothalamus (34).

    Testosterone also has effects that are manifested early in life. The neonatal organising actions of testosterone are achieved by stimulating cell growth and differentiation in the preoptic area, ventromedial hypothalamus and amygdala, and dendritic branching in the preoptic area and the hypothalamus (35). For example, authors have suggested that testosterone increases the size of the granule cell layer in the dentate gyrus (origin of hippocampal mossy fibres) and that these effects occur during a ‘critical time period’ (36,37).

    Critical time period
    The idea that there is a critical time period during which testosterone acts on the brain to sensitize certain
    neural circuits has received a great deal of attention and testosterone is now considered to have a definite role in the establishment of aggressive behaviour in adulthood (38-40). Furthermore, it appears that while testosterone modifies aggression, it does so only within a range that has been genetically programmed (41).
    In mice, male castration at birth decreased levels of fighting even in the presence of large amounts of
    testosterone propionate as adults. However, if the castration took place at ten days of age, the difference in
    fighting levels compared to controls was much less pronounced (39). Similar findings were found in
    females (16). It was concluded that androgen stimulation of the mouse before ten days of age causes
    differentiation of a neural system for aggression which is sensitive to androgens in adulthood. This agrees with others’ findings (42,43) that castration on day 0 followed shortly by a single injection of testosterone
    results in a relatively normal adult male with respect to fighting behaviour.

    Recently it has been proposed that in males during the first few days after birth, testosterone and its
    metabolites sensitize an androgen-responsive system while estrogenic metabolites organize an estrogen-responsive pathway (44). In general the organizing effect is primarily done by testosterone and its ability to
    be aromatized, and can influence either androgen- or estrogen-sensitive circuits (45). For example,
    administration of testosterone or methyltrienolone (R1881), a potent nonaromatizable androgen, on day
    one of life caused aggression which was brought on in response to androgens but not estrogens in adulthood (46). In contrast, if estrogenic metabolites were present early, then in adulthood this changed with both testosterone or estrogen able to affect aggressive behaviour in males (46).

    In females, there is also an androgen-sensitive pathway, although a greater exposure to androgens is
    required to induce male-like fighting behaviour. Evidence to support this comes from the finding that
    400 mg of testosterone propionate will effectively organise female aggression at 0-2 hours after birth but
    it will not at two days of age. However if 600 mg is given at this later stage, the treatment is effective (47).
    Furthermore, adult females are completely insensitive to the aggression-promoting effects of estrogens seen
    in males.

    Therefore there appears to be a sensitive period when the differentiation of aggression with hormones is most likely, i.e. shortly after birth, but the period can be extended by increasing the hormone dosage.
    Furthermore the results indicate that hypothalamic and associated brain mechanisms become less sensitive to exogenous androgen with time after castration (39), most likely due to decreased sensitivity of the receptors with age (48). There is, however, some conflicting evidence to suggest that the critical time period is not essential to elicit aggression in adulthood. For example, males castrated on day 0 were found to show aggression in adulthood in response to testosterone however larger doses and often a longer duration of treatment was required (16).

    Recently it has been speculated that changes also occur in utero: the testosterone secretory capacity of
    Leydig cells in the testes of mice changes differentially during intrauterine development in males of the
    aggressive and non-aggressive selection lines (49). The peak secretory capacity is reached at day 17 of gestation for the males of the aggressive selection line while the peak for the non-aggressive line is reached on the first neonatal day. Similarly female mice fought significantly sooner following the initiation of testosterone treatment in adult life if they were exposed to it between the 13 th and 18 th day of fetal development (50). Similar results were obtained by others (51,52). Coincidently, the brain aromatase activity changes during development as well (53-55); for example, at day 1, aggressive males show
    higher aromatase activity in the amygdala than in the hypothalamus, whereas in less aggressive strains the
    activity did not differ (54).

    Figure 1. The chemical structures of testosterone and its derivatives are shown. Testosterone is converted into 5a-DHT by the enzyme 5a-reductase to then act on androgen receptors. Alternatively, aromatase can convert testosterone into estradiol which acts on estrogen receptors.

    The effects of testosterone in adulthood
    While the primary action of steroid hormones is to regulate protein synthesis through a genomic action,
    they can also modulate neural activity via non-genomic mechanisms such as modifying the permeability of
    nerve cell membranes. This suggests the existence of membrane receptors for the steroid in addition to
    nuclear receptors. These non-genomic effects result in changes in electrical potential possibly through cyclic adenosine monophosphate activation and the release of neurotransmitters into the synapse (56). One of the primary actions of the steroid hormones thus may be to regulate the opening of ion channels in the cell membrane.

    It is widely accepted that steroid hormones modulate behaviour through indirect actions on neuro-transmission in the central nervous system. It is assumed that their activity is related to the density of
    steroid receptors in these areas (57). When the distribution of steroid hormone receptors in the brain is
    compared with the distribution of neurotransmitter pathways, a close relationship is observed (Figure 2);
    for example, the distribution pattern of estrogen receptors correlates well with that of noradrenaline,
    dopamine and serotonin (5-HT) nerve pathways in the rat (58). When a steroid hormone alters the electrical
    activity of a target neuron along one of these pathways the amount of neurotransmitter released from that
    neuron is altered. For example, it was found that normal fighting in mice decreased when there was an
    accelerated release of certain amines (59).

    However it should be stressed that the neural circuits underlying aggression are themselves plastic, i.e.
    neurotransmitter modulation may not be the only way testosterone can affect aggression in adulthood. For
    instance, levels of aggression tend to fluctuate during the year in many animals according to their mating
    season: when the breeding season approaches and the gonads recrude, aggression increases with a
    corresponding elevation in testosterone levels (60).

    Effects of testosterone on neurotransmitter pathways
    Many studies have been undertaken that implicate 5-HT in modulating aggression. In general, increased
    activity of serotonergic synapses inhibits aggression: studies in male rhesus monkeys showed that those with low levels of 5-hydroxyindoleacetic acid (5-HIAA) were more aggressive (30,61,62). Furthermore,
    fluoxetine, a serotonin reuptake inhibitor, tends to decrease aggression in both animals and humans. Strong
    evidence suggests that these effects are mainly mediated via 5-HT 1A and 5-HT 1B receptors (63). This is
    supported by various animal studies: 5-HT 1A agonists decrease aggression and defensive behaviour in wild rats (64); gene knockout mice lacking the 5-HT 1B receptor (equivalent to 5-HT 1Db in humans) showed greatly enhanced intruder confrontation aggression (65).

    Testosterone may also be involved in the arginine vasopressin (AVP) system, since an AVP receptor
    antagonist injected into the anterior hypothalamus of male golden hamsters was found to block their
    offensive aggression (66). This is supported by evidence that male golden hamsters have androgen-sensitive AVP receptors in the ventrolateral hypothalamus and castration leads to a loss of AVP
    binding (67). Furthermore, there appear to be 5-HT synapses on AVP neurons in the anterior hypothalamus
    and the AVP immunopositive boutons and hippocampo-septal axon terminals frequently terminate on the same somatospiny neurons on the lateral septum. Therefore, perhaps testosterone promotes aggression by lowering 5-HT levels thus removing a potential inhibitory influence on AVP.

    Testosterone has also been found to influence the density of GABA A receptors. Testosterone-treated rats
    have a decreased density of GABA A receptors in several brain regions (68) and several studies have
    implicated low levels of GABA with aggression (69,70). Coincidently, a role for GABAergic inhibition
    has been implicated in odour coding and in particular GABA A antagonists caused lowering of the response
    threshold to odours (70,71). Therefore high-attacking animals would have a better perception of the olfactory cues leading to rapid recognition of the opponent. There is also evidence to support a direct role of noradrenaline in aggression (72). Adrenoceptor blocking drugs such as phentolamine (alpha receptor
    blocker) or obsidan (beta blocker) caused a decrease in aggression and testosterone levels in dominant mice
    (73). It has been suggested that noradrenaline heteroceptors terminate on presynaptic 5-HT neurons
    raising the possibility of interaction between the two transmitter systems (72).

    Finally, testosterone has been found to suppress dopamine turnover in the anterior hypothalamus of
    male rats (74) which was thought to be involved in androgen-dependent aggression, although studies tend
    to support a positive relationship between central dopamine function and aggression (72). There have also
    been suggestions that acetylcholine and glutamate are involved in testosterone-mediated aggression.

    Research into human forms of behaviour such as aggression is complicated by the complex manner in
    which humans tend to behave. For example, studies have found that aggressive encounters are influenced by learning, i.e. the outcome of the first encounter influenced subsequent fights (75). There is also
    evidence that aggression is in itself rewarding. For example, when animals with lateral hypothalamic
    electrodes were allowed to control the stimulation, they pressed the levers with high frequencies suggesting the presence of ‘positive’ feelings (76). Interestingly, self- stimulation rates were decreased when the animals were castrated and increased again after hormone substitution.

    Other research has indicated that testosterone may be correlated with aggressive motives and competitiveness rather than violence per se. Serotonin function on the other hand may function to limit aggression to an appropriate time, setting and intensity. Overall the results indicate that while subjects with high levels of testosterone exhibit more aggression, the aggressive encounters are stopped before they escalate into dangerous forms of aggression (77). Other factors implicated in aggression include involvement of the Y chromosome (78,79), alcohol (80) and the role of olfaction (81-84).

    Figure 2. Neural circuits underlying aggression as shown in the rat brain. Original work by Hess in 1928 (26) found that ‘sham rage’ occurred after electrical stimulation of the hypothalamus. More recently, a continuous stretch of points through the hypothalamus have been mapped out in rats which when stimulated resulted in aggressive behaviour (27). Furthermore it appeared that for each form of aggression, there appeared to be different representations within the hypothalamus. For example, stimulation and lesion studies point to a strong lateral hypothalamic involvement in predatory and intermale aggression whereas the anterior hypothalamus is involved in fear-induced and irritable aggression. As such the neural systems underlying these different forms of aggression seem to converge from various parts of the brain within the hypothalamus (28). Various lesion, electrophysiological and morphological studies suggest that the neural control of aggressive behaviour, in particular the associated movements, are programmed by neural circuits in the brain stem whose activity appears to be controlled by various structures in the limbic system such as the hypothalamus and amygdala (29,30). In turn, important pathways lead from the hypothalamus through the medial forebrain bundle to the limbic cortex through the amygdala and septum. There are also connections from the olfactory bulb (31). Interestingly, high densities of androgen and estrogen receptors were found in these areas (32). Schematic diagrams show parasagittal views of the rat brain at a medial (A) and lateral (B) position. The neural circuits thought to be involved in aggressive behaviour are indicated as dotted lines. Dots represent areas found to contain androgen and estrogen receptors. Notice the overlap between the indicated neural circuits and the regions of highest androgen and estrogen receptor density.

    Most studies of testosterone and aggression have measured plasma total testosterone levels which can be
    problematic since in plasma the free fraction accessible to the central nervous system is only about 2% (85). It was therefore proposed that accurate reflection of central androgen activity might be better obtained by cerebrospinal fluid (CSF) free testosterone (77). Furthermore, investigators found that in rhesus
    monkeys testosterone was related to overall aggressiveness whereas low levels of CSF 5-HIAA
    were related to impulsivity. This has implications on a variety of studies where impulsivity was regarded as a predisposition or even a type of aggression.

    In addition, there are species differences in the structures involved in the different types of aggression.
    The cingulate gyrus is involved in fear-induced aggression in the monkey but irritable aggression in the
    cat and dog. The effects of stress are also problematic since they have been found to reduce testosterone in
    man and other animals. Therefore stress induced by defeat would naturally depress testosterone levels (86).
    Related to this, it was found that sexual stimulation (87) and exercise (88) increase testosterone levels and many of the behaviours elicited in the studies probably have an element of sexual motivation which has been shown to be estrogen dependent (89).

    Although there is strong evidence that testosterone is involved in aggression, it must be stressed that perhaps other hormones are involved. After castration, many hormone systems are disrupted which could confound the experiment. For example, aggression was more closely related to luteinizing hormone than testosterone in the male starling (90). Review and clarification of behavioural measures is therefore recommended.

    There is strong evidence in animals that testosterone is directly associated with aggression, although this
    correlation is not as strong in humans. Testosterone appears to mediate its effects during a critical time
    period shortly after birth during which it sensitizes certain neuronal circuits in the brain. As a result, in
    adulthood, when these circuits are stimulated again by steroids , aggressive behaviour is elicited perhaps
    through modulation of specific neurotransmitter pathways. However, it must be remembered that
    hormones themselves do not directly cause behaviours, but induce chemical changes in certain neurons,
    affecting the likelihood of certain behavioural outcomes as a result of modulation of particular neural pathways (91). Furthermore, gonadal hormones are only one of a myriad of influences on aggressive behaviour. Since testosterone is present in males that are not aggressive as well as in those that are, it is obvious that another factor(s) is involved, such as cognition and environmental circumstances which have been found to affect the expression of aggression (60,92).

    Scientists are limited in that they cannot perform experimental procedures in humans as they have done
    in animals and since the behaviour patterns in humans are so different from animals, rendering it difficult to
    extrapolate from animals to humans. As it was put recently “there is a danger of triviality and truism, or
    even misleading simplification, in many of our extrapolations and animal models” (76). As such we
    should remember that correlation does not necessarily indicate causation and in many studies such
    assumptions have been made. Before experiments can be done to investigate these correlations, we need to be sure that the types of aggression we are looking into are testosterone-dependent and eliminate other factors that could confound the results.

  2. #2
    Canes4Ever's Avatar
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    Sticky material, bump

  3. #3
    rager's Avatar
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    crashing my boat

  4. #4
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    Quote Originally Posted by rager
    you'll never learn anything if you dont read the long studies, those are the juicy ones =)


  5. #5
    No One Knows's Avatar
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    This **** is 3 years old..

    What the hell are you doing?

  6. #6
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    Good info. Bump this to the top!

  7. #7
    needle's Avatar
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    CeN CAL
    I think if u have taken juice you would no this allready!!!

  8. #8
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    Quote Originally Posted by rager

    Way to bump a nearly 3 year old thread and then bitch about it Bro

  9. #9
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    South JerseyJ
    Thanx for the bump Great read

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    Bumpity Bump...

  11. #11
    Hed is offline Senior Member
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    Great read, but all studies done in animals.

    Also, being a 4 year old study, they now probably think steroids have a high likelihood of shooting bananas out your ass or something totally different like that.

  12. #12
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    Quote Originally Posted by needle
    I think if u have taken juice you would no this allready!!!

  13. #13
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    Quote Originally Posted by powerlifter
    Way to bump a nearly 3 year old thread and then bitch about it Bro

  14. #14
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  15. #15
    rager's Avatar
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    Quote Originally Posted by ArkansasAlex
    you'll never learn anything if you dont read the long studies, those are the juicy ones =)

    I did read... nice .. just long

  16. #16
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    Good post. It was a lot to read though.BUMP

  17. #17
    rager's Avatar
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    Quote Originally Posted by needle
    I think if u have taken juice you would no this allready!!!

    lol no kidding? who doesnt know this S?

  18. #18
    rager's Avatar
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    Quote Originally Posted by powerlifter
    Way to bump a nearly 3 year old thread and then bitch about it Bro
    what the f.. I didnt post this bro?

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