Can you pressure cook or boil a sealed vial?

[PistolPete33]

If I decide to cycle again I want to take every precaution possible to ensure I never get an infection. I was thinking if I could pressure cook or boil sealed vials I would kill off any possible bacteria and also filter the oil before injecting to ensure no particles are getting thru. Is this possible with rubber stoppers because wouldn't they melt?

[clarky.]

You could get some stetile vials then filter you oil in to them them pressure cook them i can't remember at what psi i think it's about 15 psi it's for about 45 min and it doesn't melt the rubber

[ScotchGuard02]

If you filter the gear through a .22um whatman syringe filter into a sterile vial you should be good to go. I you want to pressure cook that good too but I don't think it's necessary.

[Bonaparte]

Yes. I pressure cook all my sealed vials.

[PistolPete33]

Yes. I pressure cook all my sealed vials.

Would you mind sharing how you accomplish this? Thanks in advance.

[Lemonada8]

you can also boil them.. a procedure called 'tantalizing"

[APM]

If you filter the gear through a .22um whatman syringe filter into a sterile vial you should be good to go. I you want to pressure cook that good too but I don't think it's necessary.

If you want to be extra cautious this is what I would do.. Filter the gear through a .22um syringe filter into another presealed sterile vile..

You can use a pressure cooker to do the final sterilization. 15psi for around 30 min. I prefer a pressure cooker that has a metal tray inside that keeps the contents in there elevated slightly above the water..

Hit the lab section and do some searching, lots of info on this subject...

[Bonaparte]

you can also boil them.. a procedure called 'tantalizing"

That would be Tyndallization (named after the scientist John Tyndall).
But it requires 3 separate boils and is probably less effective than autoclaving.

[Lemonada8]

^^ thanks, i figured i spelled it wrong..

and how many people have access to a autoclave?


like it was said above, check out the lab section.. lotta info there

[Java Man]

That would be Tyndallization (named after the scientist John Tyndall).
But it requires 3 separate boils and is probably less effective than autoclaving.

I think he was thinking about how tantalizing all that test looks together in one pot.

[Bonaparte]

^^ thanks, i figured i spelled it wrong..

and how many people have access to a autoclave?


like it was said above, check out the lab section.. lotta info there

It's just a pressure canner. You can buy them at any department store for a reasonable price.