reconstitution of Igf-1 Lr3

**kburek** · 02-07-2007, 06:07 PM

I just bought recently few 1mg vials of igf1 Lr3.I also have AA (0,6%).My question is hom many ml of AA should I inject in 1mg igf.1 vial???

**ecivon** · 02-07-2007, 07:19 PM

Originally Posted by kburek

I just bought recently few 1mg vials of igf1 Lr3.I also have AA (0,6%).My question is hom many ml of AA should I inject in 1mg igf.1 vial???

I use bw. I draw 1 ml bw and add it to the igf vial slowly. Let it run down the side of the vial and the igf will dissolve immediately. You can swirl it a little to make certain it all dissolves. I then remove all of the solution and transfer it to a sterile vial and then add one more cc of bw. Note: if you got it from ARR then you don't have to transfer to another vial as they ship the igf in a 10 ml vial, just use it. When you are done you will have 500mcgs/ml. This is all I dilute it. Some guys will add enough to make 10ml, but I don't see any point in it. 2 ml is plenty. So you have 500 mcgs/ml. On a slin pin then four marks equals 40mcgs.

Good stuff -- I really like igf.

**longhorn814** · 02-07-2007, 09:39 PM

do NOT used BW to reconstitute your IGF-1. You need to use AA, BW will degrade your IGF. You reconstitute with AA and when you inject you draw some BW out of a vial and then draw in the appropriate amoutn of IGF into the same syringe and inject. The BW will help reduced the sting of AA

**kburek** · 02-08-2007, 03:48 AM

Originally Posted by longhorn814

do NOT used BW to reconstitute your IGF-1. You need to use AA, BW will degrade your IGF. You reconstitute with AA and when you inject you draw some BW out of a vial and then draw in the appropriate amoutn of IGF into the same syringe and inject. The BW will help reduced the sting of AA

I already bought AA from this website
http://researchlabsupply.com/Scripts...?idproduct=254
Should I use then only this AA to reconstitution Igf1Lr3?Or I have to use BW as well?I read that Bw kills Igf-1 that's why I bought AA.My question is can I use only AA for my Igf-1 cycle or I just use it for partial reconstitution?and how many ml of AA should I use for 1mg vial of Igf-1? thanks

**kburek** · 02-08-2007, 09:35 AM

Can I for example dilute 1mg vial of Igf-1 only with 5ml of AA and use it like this for injection?Or it's better to dilute 1mg with 2,5ml of AA and then dilute in the slin syringe with BW in proportion 50/50?(that way every 0,25ml is 50 mcg of igf-1)

**longhorn814** · 02-08-2007, 01:12 PM

I use 2 cc of AA to dilute IGF-1 so every 5 mark on the slin pin is 5 mcg. I typical draw up to 10-15 units of BW and then draw up 25 mcg of IGF into the same pin and inject. The BW is just to reduce the sting, although it doesnt help alot. You can just inject the IGF without the BW if you want, but I like to do it this way b/c it ensure that all the IGF gets pushed through the pin

**fossilfuel7** · 02-08-2007, 01:55 PM

Well....I don't know if it is the same for IGF as GH...but, in REDBARON'S guide to HGH... he says to reconstitute with BW and nothing about AA...unless I am reading it wrong.

**plzr8** · 02-08-2007, 02:03 PM

Originally Posted by fossilfuel7

Well....I don't know if it is the same for IGF as GH...but, in REDBARON'S guide to HGH... he says to reconstitute with BW and nothing about AA...unless I am reading it wrong.

from one of RB's stickys....

"For Long R3 IGF-1, it isn’t as critical that you inject into a local site as long R3 has a active window of many hours, and is designed specifically to resist being bound by IGF binding proteins.

Since it is common to reconstitute this type of IGF-1 with Benzyl Alcohol, Acetic Acid, or Hydrochloric Acid, I would still recommend that you inject intra-muscular. While for some purposes of nerve regrowth, etc. subQ is a somewhat superior injection method, it can and probably will leave a nice red irritated spot if you inject subQ, and it is not superior for muscle growth purposes anyway."

acetic acid is used and preferred by many sources & users

**ecivon** · 02-08-2007, 02:18 PM

Originally Posted by plzr8

from one of RB's stickys....

"For Long R3 IGF-1, it isn’t as critical that you inject into a local site as long R3 has a active window of many hours, and is designed specifically to resist being bound by IGF binding proteins.

Since it is common to reconstitute this type of IGF-1 with Benzyl Alcohol, Acetic Acid, or Hydrochloric Acid, I would still recommend that you inject intra-muscular. While for some purposes of nerve regrowth, etc. subQ is a somewhat superior injection method, it can and probably will leave a nice red irritated spot if you inject subQ, and it is not superior for muscle growth purposes anyway."

acetic acid is used and preferred by many sources & users

I've tried to find info on this and emailed Lion for clarification. Some people told me BW which I used. Lion ships theirs with sodium chloride, SP used BA and I believe MR used AA, or BA. I can't find anything conclusive and would like to hear something definitive.

**plzr8** · 02-08-2007, 02:45 PM

Originally Posted by ecivon

I've tried to find info on this and emailed Lion for clarification. Some people told me BW which I used. Lion ships theirs with sodium chloride, SP used BA and I believe MR used AA, or BA. I can't find anything conclusive and would like to hear something definitive.

AA to reconstitute, sodium chloride or BW to purge/inject

thats the majority of what i read & do.....and MR does use AA

**kburek** · 02-08-2007, 05:24 PM

Originally Posted by longhorn814

I use 2 cc of AA to dilute IGF-1 so every 5 mark on the slin pin is 5 mcg. I typical draw up to 10-15 units of BW and then draw up 25 mcg of IGF into the same pin and inject. The BW is just to reduce the sting, although it doesnt help alot. You can just inject the IGF without the BW if you want, but I like to do it this way b/c it ensure that all the IGF gets pushed through the pin

I asume that you dilute 100mcg vial of Igf-1 with 2cc (2ml )of AA.That's quite a lot I think.

**kburek** · 02-08-2007, 05:32 PM

Originally Posted by fossilfuel7

Well....I don't know if it is the same for IGF as GH...but, in REDBARON'S guide to HGH... he says to reconstitute with BW and nothing about AA...unless I am reading it wrong.

According to RedBaron recipe,which I found on this froum:

"I believe you posted this (someone reposted it) regarding how to arrive at that final 0.6% (100mM) Acetic Acid:
***************************
Reconstituting IGF-1 with white vinegar

I have noticed several posts lately wondering about a good method for reconstituting IGF-1 and a few posts about using vinegar/water to do so. I originally wrote this post at ****************, but since there seems to be a reasonable interest here as well, I will post it for those that might be interested.

I began using this method many months ago mainly out of impatience. I was out of Benzyl Alcohol, and while I had access to HCl, it wasn't accessible when I wanted to begin my IGF-1 LR3 cycle. I decided to use plain ol' grocery store Distilled White Vinegar. I used the following, which has worked out really well for me....and the feedback I have had from others that I have shared it with has been positive so far. So here it is for anyone that is interested:

The calculation:
Distilled white vinegar is supposed to be standardized to ~5% acetic acid, which would make it 850mM. To get it to the recommended 100mM, you'd want 11.76% white vinegar (100mM/850mM = 11.76%). Since it would be almost impossible to draw out 11.76IU's, I round this to 12, which is certainly going to be close to our desired 100mM.

The filtering process:
I use off the shelf grocery store distilled white vinegar. In order to ensure safety, I filter it using .20u whatman filters. Here is the step by step for those that may not be familiar with filtering using whatmans. What you will want to have on hand before starting out is some sterile vials, some .20u whatman filters, some syringes and needles (I use a 10cc syringe, and .23 gauge 1" needles), and some alcohol swabs.

(1) First draw up about 10cc of the distilled white vinegar
(2) screw on the .20u whatman to the 10cc syringe (or whatever size you use)
(3) screw on a .23 gauge needle (or whatever size you decide to use)
(4) take your sterile vial, swab the top with alcohol, insert a needle for venting.
(5) Insert your syringe/whatman/needle apparatus and slowly push the 10cc's into the sterile vial.

Now you have safe vinegar to use for your reconstituting.

Reconstituting:

How much water/vinegar you reconstitute with is going to somewhat depend on which IGF-1 LR3 you are using. Igtropin is shipped in 100mcg vials, which I usually reconstitute at 1ml(cc) per 100mcg vial. The gropep based IGF-1's are primarily shipped in 1mg vials, and I usually use 3ml - 5mls for these. At any rate, what I do is:

(1) take an alcohol swab and swab the tops of my water, vinegar solution, and IGF-1 vials
(2) take a 3cc syringe with a 23 gauge, 1" needle and draw out .12 cc's of vinegar for the 100mcg vials or .36 cc's(if 3ml) - .60cc's(if 5ml) for the 1mg vials.
(3) next I take this syringe and draw out the water - .88cc's for 100mcg, 2.64cc's(if 3ml) to 4.4cc's(if 5ml) for the 1mg.
*****I realize that I can't get this kind of precision, but I get it as close as possible******
(4) next i poke the needle into the IGF-1 LR3 vial and dribble this solution down the side of the vial, avoid any direct spray on the lyophilized powder until all of the dilutent is in the vial
(5) using a gentle swirling motion, I reconstitute the powder.
(6) I stick the vial in the fridge and it is now ready for use.

Well, I think that about sums it up. Hope this helps some people who have maybe been wondering about using vinegar to reconstitute. I would advise that if you end up using Igtropin, you avoid using BA....use this vinegar method for sure. Igtropin and BA do not get along well together at all."

RedBaron

So I think if I bought ready 0,6% AA solution I don't need to use BW for futher dilution.Or I can use it only to reduce the sting right?
Any advice?

**longhorn814** · 02-08-2007, 06:26 PM

Originally Posted by kburek

I asume that you dilute 100mcg vial of Igf-1 with 2cc (2ml )of AA.That's quite a lot I think.

no i dilute 1000 mcg of IGF with 2 cc (ml) of AA

**kburek** · 02-08-2007, 06:36 PM

Originally Posted by longhorn814

no i dilute 1000 mcg of IGF with 2 cc (ml) of AA

but that means that 2cc(2ml)of solution has 1000mcg,then 1ml=500mcg.If there is 50 marks on slin pin (equals 100 units) that means every 5 marks is 50 mcg, not 5 mcg.

**longhorn814** · 02-08-2007, 07:27 PM

1000mcg/2ml = 50mcg/X if you solve this equation X = 0.1 ml which is the 10 mark on a slin pin.

That was a typo in my earlier post. I meant every 5 mark is 25mcg. I use 1/2 cc insulin syringes so it only numbers up to 50 or 0.5 cc. It makes dosing much more accurate than 100 unit pins

**kburek** · 02-09-2007, 12:42 PM

Originally Posted by longhorn814

1000mcg/2ml = 50mcg/X if you solve this equation X = 0.1 ml which is the 10 mark on a slin pin.

That was a typo in my earlier post. I meant every 5 mark is 25mcg. I use 1/2 cc insulin syringes so it only numbers up to 50 or 0.5 cc. It makes dosing much more accurate than 100 unit pins

Oh yeah...now it's fine, I mean if 5 mark is 25 mcg of Igf-1.
Thanx
BTW what kind of AA are you using ??Is it 0,6% solution??