Recombinant Human Myostatin Propeptide (Myostatin Human)

[PK-V]

Source: E.Coli


Introduction:

Myostatin (GDF-8), a member of the TGFbeta superfamily, is a potent and specific negative regulator of
skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin
propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in
vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide.
The myostatin propeptide is negative regulator of myostatin in vivo.


Description:

28 kDa protein containing 243 amino acid residues of the human Myostatin Propeptide and 5 additional
amino acid residues.
MG NENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI
DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR
PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD
ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN


Synonyms:

GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.


Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.


Specificity:

The amino acid sequence of the recombinant human Myostatin Propeptide is 100% homologous to the
amino acid sequence of the human Myostatin Propeptide without signal sequence.


Purification Method:

Two-step procedure using size exclusion chromatography before and after refolding.


Purity:

>95% (SDS-PAGE analyzed).


Formulation:

Sterile filtered and lyophilized from 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 8.0.


Solubility:

Add 0.2 ml of d H2O and let the lyophilized pellet dissolve completely.


Stability:

Store lyophilized protein at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing
cycles. Reconstituted protein can be stored at 4 °C for a limited period of time.
The lyophilized protein remains stable until the expiry date when stored at -20 °C.


Applications:

Western blotting, ELISA


Usage:

Product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs,
agricultural or pesticidal products, food additives or household chemicals.

[PK-V]

SO WHO WANTS TO BE THE FIRST TO TRY IT OUT!?

l

[dece870717]

Damn I'd be up for it sort of but don't feel like killing myself.

[dece870717]

Googled it and there seems to be places to get it, but looks pricey as hell, well depends on i guess how much you would actually need to use to get the benefits from it.

[PK-V]

Googled it and there seems to be places to get it, but looks pricey as hell, well depends on i guess how much you would actually need to use to get the benefits from it.

Truth

I wonder if there are any logs out their, surely someone out there has tried it!?

[dece870717]

Heres some interesting studies - http://www.ncbi.nlm.nih.gov/pubmed/2...m&ordinalpos=1

http://www.ncbi.nlm.nih.gov/pubmed/1...m&ordinalpos=5

[redz]

While it does sound very interesting it remains to be seen what the effects on humans would be. I would be interested if anyone is crazy enough to try it but at this point that could be risky.

[dece870717]

While it does sound very interesting it remains to be seen what the effects on humans would be. I would be interested if anyone is crazy enough to try it but at this point that could be risky.

Well so far there are 2 kids that have been documented to have a genetic myostatin defect. The one child in Germany that has a complete myostatin knockout deficiency and then there's the one boy in Michigan that produces normal amounts of myostatin but for whatever reason myostatin doesn't work. The only side effects they seem to have is having a lot of muscle and no fat. I don't see what serious side effects would happen from blocking something that controls only skeletal muscle growth.

[redz]

I`m not saying it would be dangerous but there could be other un-known side effects.