Riptropin vial testing results

[Sheven]

I will repeat one last time which is quoted from the concerns thread and this thread, the email is form an expert. You cant test for the hormone to be active.........If you have read the full concernc threads and you still dont see it or understand i'm wasting my time and effort with you, you carry on taking them dont worry what thwe vets and guys who have been using generics and pharm for over 20 yrs say, adios




The process to produce rHGH is very complicated and the equipment used cost's millions of dollars and many of the generic companies don't have this equipment, this is why to produce rHGH it costs a lot of money due to the expensive equipment need to complete the process. I do know someone who I am in contact with and in one of his first emails to me he describes the process of testing this horomone. I will copy the email so you get more of an understanding. My contact has many titles, he is a Professor of research, Scientific manager, he is head of the Biopharmaceutical Bioprocessing technology centre, Director of Mass spectrometry in clinical pharmacology who provide protein analysis services to commercial and academic clients and also a Doctor



Hi Marcus,


there are some things that we can do, some things that we could do, but that would possibly be too expensive (not worth it) and other things that we cannot do.


Please let me start with the last section:


We absolutely cannot test any substances for their suitability for any purpose, particularly not for their use in a diagnostic, therapeutic and/or recreational purpose, when this invloves administering to a living being (including humans).


Measuring the concentration of the growth hormones is something that is actually not as easy as it might seem. The concentration of the hormone can have two different meanings, it could be the chemical concentration of a compound (this is something that we can measure), it could also be the biological activity of this compound (this would be different, if a certain proportion of this chemical would be biologically inactive, which could happen for a variety of reasons). The latter (measuring the biological activity) is something we cannot do. We can measure the chemical concentration of human growth hormone (or any other growth hormone ), but that would involve the chemical synthesis of an internal standard, followed by a fairly complicated experiment, in which we use chemical scissors (en enzyme called trypsin) to chop the protein into pieces, then we analyse these pieces using a combination of high performance liquid chromatography and mass spectrometry, followed by a computer-intensive analysis of the data. The result of this experiment would be the concentration either in units of mol/l or in units of mg/l (the two are interconvertible). I do not know how the IU (stands for International Units) for growth hormone are defined - this might be a functional (biological) unit (which we cannot measure).


Human growth hormone is a chain of 191 different amino acids. One thing we can do is measure the total molecular weight of the protein in a sample to see, if it corresponds to the molecular weight that would be predicted for a protein containing these 191 amino acids. This experiment detects, if one amino acid was missing or another one was added, in some cases (but not in all cases) even, if an amino acid was replaced with a different one. Another outcome of this experiment would be to see, if other, similar proteins are present in the same sample.


In a different experiment we can check, if a protein in a particular sample is really growth hormone or if it is possibly something completely different, for example egg white protein or milk protein .


For your information, I attach two links to articles about human growth hormone :



Best Wishes

The suitability is established during 3 clinical trial phases, but that's mostly for side effects and latent effects. For positive effects its not that hard to prove, this kind of tests are the bio-equivalence ones.

Marcus: as to how to analyze somatropin, i'm doing this for over 4 years now and it ain't that complicated as it seems as long as you can understand the purpose of each analysis. as i said, PMF with PeptideMap is compared the peptide masses from the spectra, for the molecular weight is done the matrix assisted laser desorption ms (maldi-ms) with the mass range use of 10k-65k daltons, to determine the n-terminal sequencing is used the prosorb filter and then put in the cartridge of a protein sequencer and performed the automated edman sequencing. sds page can also be used as a primitive method to complete the whole job (this can be done by anyone in its home). for the PMF is used trypsin however here is often the case that because of the autoproteolitic digest of the enzyme a large number of the peptide masses are not explained. but all this is according to the Ph.Eur so it can pass the analysis even without those masses explained.

first of all, people that want to get into this discussion and argue on this subject should read the monograph of somatropin then understand it and then we can get into more details but i doubt we can really achieve a common ground because it would take some serious study and here is a BB forum not a bio chemist lab section.

[marcus300]

http://forums.steroid.com/showthread...T#.UMzIQ6zX_fs

The suitability is established during 3 clinical trial phases, but that's mostly for side effects and latent effects. For positive effects its not that hard to prove, this kind of tests are the bio-equivalence ones.

Marcus: as to how to analyze somatropin, i'm doing this for over 4 years now and it ain't that complicated as it seems as long as you can understand the purpose of each analysis. as i said, PMF with PeptideMap is compared the peptide masses from the spectra, for the molecular weight is done the matrix assisted laser desorption ms (maldi-ms) with the mass range use of 10k-65k daltons, to determine the n-terminal sequencing is used the prosorb filter and then put in the cartridge of a protein sequencer and performed the automated edman sequencing. sds page can also be used as a primitive method to complete the whole job (this can be done by anyone in its home). for the PMF is used trypsin however here is often the case that because of the autoproteolitic digest of the enzyme a large number of the peptide masses are not explained. but all this is according to the Ph.Eur so it can pass the analysis even without those masses explained.

first of all, people that want to get into this discussion and argue on this subject should read the monograph of somatropin then understand it and then we can get into more details but i doubt we can really achieve a common ground because it would take some serious study and here is a BB forum not a bio chemist lab section.

The email was from a guy who's been the heasd in this field for over 20yrs in the UK and ive also spoken to 2 other professionals who state test for the hormone to be active is extremely hard to do and even the Chinese told me this after months of arguing over emails with Ankebio..

Many people have agenders on here for stating such claims so I think we are getting into delicate area......

I was a huge buyer of generics and infact ive bought ten of thousands of ius over the years and the guys who I were dealing with were some of the top suppilers in China and we had a great relationship and he told me many things what the chinese are doing with the generic underground market and thats why I will not even paid to put that crap into my body again