Thread: Frozen Long R3 Igf
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04-04-2006, 07:32 AM #1New Member
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Frozen Long R3 Igf
Research-ology Long R3 IGF is what I am working with. I mixed it correctly and put it in the refrig. Someone cut the fridge down to almost freezing and now my stuff is frozen but thawing. The instructions said do not freeze.. Hope I did not f-this up... Anyone ?
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04-04-2006, 01:21 PM #2
If it says "do not freeze" and you froze it, there's no guarantee that it's good
JohnnyB
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04-04-2006, 09:56 PM #3
It should be fine it has a life of over a year at -180
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04-05-2006, 06:52 AM #4New Member
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Dose Question
I have 1000mcg (after diluted in sodium chloride) of Research Ology Long R3 IGF. My question is on the insulin pins how much should i draw out to get 25mcg. It is listed from 10-100 and it is a one cc syringe. Help with dosage someone. Got this stuff and want to get the right dosage ?
Thanks
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04-05-2006, 07:50 AM #5
storage
Storage of LR3 IGF-1
*Study conducted by Gropep
*posted by LakeMounted @ *************.com
The stability of a liquid solution of LR3IGF-I was monitored for a period of two years at storage conditions of -20 C, +4 C, +22 C, and +37 C. The final concentration of LR3IGF-I was in acetic acid. At various time points, samples were taken and compared to a lyophilized control (stored at 4 C). Listed below are the stability results for each respective storage condition.
Storage Condition: -20 C (-4 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +4 C (39.2 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +22 C (71.6 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +37 C (98.6 F)
Biological Potency No Change up to 1 year
Immunological Activity No Change up to 1 year
Mobility of Protein No Change up to 1 year
Elution Profile by reversed phased HPLC No Change up to 1 year
In conclusion, there is no significant difference in the potency of LR3IGF-I associated with the storage of the liquid formulation when stored at this range of temperatures. There is no evidence for loss of biological activity at any of the tested temperatures when stored as a liquid product. As you can see IGF can be quite stable for even a year at room temperature, but if you want to keep it around for a while then stick it into the fridge. So in my opinion the best way to store LR3 that is suspended in BA (benzoic acid) is in the freezer. The BA won’t allow it to freeze. And if you have it suspended in AA (acetic acid), store it in the fridge.
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04-05-2006, 10:02 PM #6
great post ....
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04-05-2006, 11:39 PM #7Banned
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Haha, yea thanks for taking the credit of what I posted! haha I j/k I know you gave me credit.
Although I question the fact that you have put your LR3 into NaCl solution considering Gropep insists to use BA or AA due to pKa (relative acidity) reasons.
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04-06-2006, 04:02 PM #8
who
Originally Posted by LakeMountD
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11-28-2006, 02:18 PM #9
Whew! Just froze mine, I feel much better!
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11-28-2006, 03:58 PM #10Junior Member
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Very good post. This should help out a few people.
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11-28-2006, 08:08 PM #11Associate Member
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why does everyone say this stuff must be refridgerated then?
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11-30-2006, 06:10 PM #12Associate Member
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anyone??
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