Closed

[the_rebirth]

3DS what's that avy about??

It means Vizual Xcellence.. Another one of Swolecats companies.. Back in 2001 I was a complete fatass / going nowhere, hated everything, met Swole.. got on a program, helped him out w/his early days of the internet (back when they didnt like him here.. just thought he was a big ad..lol) and changed my whole life around.. without having the vizual xcellence of my own self i could not go forward with anything in my life. but now that i have vizual xcellence of my mind and body im able to achieve my wildest dreams

the picture is actullay my tatto on my right forearm like 1hr after i got it.

i like it on the forearm for those days that are not soo good i can look at it and remind myslef what im doing

[novastepp]

really cool man. respect.

[the_rebirth]

really cool man. respect.

Thank ya!

[novastepp]

man can we get this to 400 pages so i can sleep tonight?

[novastepp]

Swole is a great teacher. i have only typed with him casually, but his advice is always taken to heart. he knows his shit and i always have questions i want to ask but my ignorance stops me... haha

[novastepp]

400 come on.

[novastepp]

so i'm a booty man...

[novastepp]

time for a shower!

400 when i get back?

[kloter1]

so shave your face with mace in the dark

[kloter1]

im a loser baby so why dont you kill me

[novastepp]

everybody...

[novastepp]

prayed for God to bring you near, along with all these tears..
and that's what wrong...
that's what i get...
for feelin this, anyway...
just let me know...
all i'll miss...
when i'm gone...

[novastepp]

just throw it all away...





you let me down.

[novastepp]

there's nothing to be afraid of.
virtual reality will rehabilitae your mind and eventually your body.
you'll be alright, i promise.
just concentrate.
and trust the music.

[novastepp]

fisted! just the way your mom likes it.

[stunner5000pt]

why cant ppl just act normal so no one has to get banned

[novastepp]

can we please get to 400 pages...

[novastepp]

why cant ppl just act normal so no one has to get banned

what u referring to?

[stunner5000pt]

ahhh i ve been talking to this girl or a while and im startingto get annoyed with her self deprecating talk... ALL THE FU CKING TIME

o im ugly
o you dont find me hot

and we're not even dating

i need to find someone who's better than her

[novastepp]

a certain thread u might have me look at?

[novastepp]

ahhh i ve been talking to this girl or a while and im startingto get annoyed with her self deprecating talk... ALL THE FU CKING TIME

o im ugly
o you dont find me hot

and we're not even dating

i need to find someone who's better than her

i'm kind of there right now with my girl. only we're dating and its a crap shoot on wether or not teh day is going to go ok...


i shouldn't have gotten myself into the situation anyway...

[novastepp]

finally to 400 pages. have a good night stunner... i'll add you to my list of bros to pray for.

[stunner5000pt]

finally to 400 pages. have a good night stunner... i'll add you to my list of bros to pray for.

i wll mate

goodnight

[the_rebirth]

w00t four double zero

[PhishStasH]

this thread wont make it past 3 or 4 pages.

Wrong!

[PhishStasH]

i'm kind of there right now with my girl. only we're dating and its a crap shoot on wether or not teh day is going to go ok...


i shouldn't have gotten myself into the situation anyway...

Def same deal with myself and my girl. Days we don't argue are wonderful, but if we get into it. I usually just don't wanna talk to her, see her, or acknowledge her existence until I forget about whatever it was we got into it over in the first place.

Luckily...it's getting better. We've been together 3+ yrs so I'm glad it is gettin' better, cause I don't wanna walk away from something I put so much effort into.

[stunner5000pt]

Def same deal with myself and my girl. Days we don't argue are wonderful, but if we get into it. I usually just don't wanna talk to her, see her, or acknowledge her existence until I forget about whatever it was we got into it over in the first place.

Luckily...it's getting better. We've been together 3+ yrs so I'm glad it is gettin' better, cause I don't wanna walk away from something I put so much effort into.

at least you dont give up

i know i will ... in time... with this girl

[PhishStasH]

at least you dont give up

i know i will ... in time... with this girl

If you are positive it's not gonna last..why still takin' her out to dinner and wastin' your time and money and shit, man? DROP her. Go find someone else. Anyhow, good luck. It can be really hard, some times. The good ones are out there, though. Even though they are usually dating f*cking assholes.

[stunner5000pt]

If you are positive it's not gonna last..why still takin' her out to dinner and wastin' your time and money and shit, man? DROP her. Go find someone else. Anyhow, good luck. It can be really hard, some times. The good ones are out there, though. Even though they are usually dating f*cking assholes.

its easier than taht ... actually

[Myka]

just pull the trigger suzy

[stunner5000pt]

just pull the trigger suzy

i wish i could

[Myka]

need some help?

[Myka]

only the lonely...know the way I feel tonight

[PhishStasH]

just pull the trigger suzy

Be sure to remove gun from pants BEFORE doing so. Nice going MC Cheddar.

[ShnouzedUp]

what up ho bags

[ShnouzedUp]

happy birthday NOOOVAAA

[ShnouzedUp]

sorry im a day late

[PhishStasH]

Ah, crap. Time to start studying Microbiology. Exam in 11 hours...

[peump]

Yersinia pseudotuberculosis and Yersinia pestis are more resistant to bactericidal cationic peptides than Yersinia enterocolitica

JA Bengoechea, B Lindner, U Seydel, R Diaz and I Moriyon
Departamento de Microbiologia, Universidad de Navarra, Pamplona, Spain.

The action of bactericidal polycationic peptides was compared in Yersinia spp. by testing peptide binding to live cells and changes in outer membrane (OM) morphology and permeability. Moreover, polycation interaction with LPS was studied by measuring the dependence of dansylcadaverine displacement and zeta potential on polycation concentration. When growth at 37 degrees C, Yersinia pestis and Yersinia pseudotuberculosis bound less polymyxin B (PMB) than pathogenic or non-pathogenic Yersinia enterocolitica, regardless of virulence plasmid expression. Y. pseudotuberculosis OMs were unharmed by PMB concentrations causing extensive OM blebbing in Y. enterocolitica. The permeability to lysozyme caused by PMB was greater in Y. enterocolitica than in Y. pseudotuberculosis or Y. pestis and differences increased at 37 degrees C. Similar observations were made with other polycations using a polymyxin/novobiocin permeability assay. With LPS of cells grown at 26 degrees C, polycation binding was highest for Y. pseudotuberculosis and lowest for Y. pestis, with Y. enterocolitica yielding intermediate results which were lower for pathogenic than for non-pathogenic strains. With LPS of cells grown at 37 degrees C, polycation binding remained unchanged for Y. pestis and pathogenic Y. enterocolitica, increased for non-pathogenic Y. enterocolitica and decreased for Y. pseudotuberculosis to Y. pestis levels. Polycation binding related in part to differences in charge density (zeta potential) of LPS aggregates, suggesting similar effects at bacterial surfaces. It is suggested that species and temperature differences in polycation resistance relate to infection route, invasiveness and intracellular multiplication of Yersinia spp.

[peump]

there you should be fine now